Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues

Anita Badbaran; Reiner K Mailer; Christine Dahlke; Jannis Woens; Anahita Fathi; Sibylle C Mellinghoff; Thomas Renné; Marylyn M Addo; Kristoffer Riecken; Boris Fehse

doi:10.1016/j.omtm.2021.10.002

Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues

Standard

Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues. / Badbaran, Anita ; Mailer, Reiner K; Dahlke, Christine; Woens, Jannis; Fathi, Anahita; Mellinghoff, Sibylle C; Renné, Thomas ; Addo, Marylyn M ; Riecken, Kristoffer ; Fehse, Boris.

In: MOL THER-METH CLIN D, Vol. 23, 10.12.2021, p. 418-423.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Badbaran, A , Mailer, RK, Dahlke, C, Woens, J, Fathi, A, Mellinghoff, SC, Renné, T , Addo, MM , Riecken, K & Fehse, B 2021, 'Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues', MOL THER-METH CLIN D, vol. 23, pp. 418-423. https://doi.org/10.1016/j.omtm.2021.10.002

APA

Badbaran, A., Mailer, R. K., Dahlke, C., Woens, J., Fathi, A., Mellinghoff, S. C., Renné, T., Addo, M. M., Riecken, K., & Fehse, B. (2021). Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues. MOL THER-METH CLIN D, 23, 418-423. https://doi.org/10.1016/j.omtm.2021.10.002

Vancouver

Badbaran A , Mailer RK, Dahlke C, Woens J, Fathi A, Mellinghoff SC et al. Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues. MOL THER-METH CLIN D. 2021 Dec 10;23:418-423. https://doi.org/10.1016/j.omtm.2021.10.002

Bibtex

@article{7871c82b605b4f74a6407cc1af4f63a4,

title = "Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues",

abstract = "Vaccination with the adenoviral-vector-based AstraZeneca ChAdOx1 nCov-19 (Vaxzevria) vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector in vivo represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia (VITT). We previously have shown that digital PCR (dPCR) is an excellent tool to quantify transgene copies in vivo. Here, we present a highly sensitive dPCR for in situ quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human plasma 24, 72, and 168 h post vaccination and in a variety of murine tissues in an experimental vaccination model 30 min post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.",

author = "Anita Badbaran and Mailer, {Reiner K} and Christine Dahlke and Jannis Woens and Anahita Fathi and Mellinghoff, {Sibylle C} and Thomas Renn{\'e} and Addo, {Marylyn M} and Kristoffer Riecken and Boris Fehse",

note = "{\textcopyright} 2021 The Author(s).",

year = "2021",

month = dec,

day = "10",

doi = "10.1016/j.omtm.2021.10.002",

language = "English",

volume = "23",

pages = "418--423",

journal = "MOL THER-METH CLIN D",

issn = "2329-0501",

publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues

AU - Badbaran, Anita

AU - Mailer, Reiner K

AU - Dahlke, Christine

AU - Woens, Jannis

AU - Fathi, Anahita

AU - Mellinghoff, Sibylle C

AU - Renné, Thomas

AU - Addo, Marylyn M

AU - Riecken, Kristoffer

AU - Fehse, Boris

PY - 2021/12/10

Y1 - 2021/12/10

N2 - Vaccination with the adenoviral-vector-based AstraZeneca ChAdOx1 nCov-19 (Vaxzevria) vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector in vivo represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia (VITT). We previously have shown that digital PCR (dPCR) is an excellent tool to quantify transgene copies in vivo. Here, we present a highly sensitive dPCR for in situ quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human plasma 24, 72, and 168 h post vaccination and in a variety of murine tissues in an experimental vaccination model 30 min post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.

AB - Vaccination with the adenoviral-vector-based AstraZeneca ChAdOx1 nCov-19 (Vaxzevria) vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector in vivo represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia (VITT). We previously have shown that digital PCR (dPCR) is an excellent tool to quantify transgene copies in vivo. Here, we present a highly sensitive dPCR for in situ quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human plasma 24, 72, and 168 h post vaccination and in a variety of murine tissues in an experimental vaccination model 30 min post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.

U2 - 10.1016/j.omtm.2021.10.002

DO - 10.1016/j.omtm.2021.10.002

M3 - SCORING: Journal article

C2 - 34786434

VL - 23

SP - 418

EP - 423

JO - MOL THER-METH CLIN D

JF - MOL THER-METH CLIN D

SN - 2329-0501

ER -