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Diffusion-driven device for a high-resolution dose-response profiling of combination chemotherapy

Standard

Diffusion-driven device for a high-resolution dose-response profiling of combination chemotherapy. / Ganser, Alexander; Roth, Günter; van Galen, Joost C; Hilderink, Janneke; Wammes, Joost J G; Müller, Ingo; van Leeuwen, Frank N; Wiesmüller, Karl-Heinz; Brock, Roland.

In: ANAL CHEM, Vol. 81, No. 13, 01.07.2009, p. 5233-40.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ganser, A, Roth, G, van Galen, JC, Hilderink, J, Wammes, JJG, Müller, I, van Leeuwen, FN, Wiesmüller, K-H & Brock, R 2009, 'Diffusion-driven device for a high-resolution dose-response profiling of combination chemotherapy', ANAL CHEM, vol. 81, no. 13, pp. 5233-40. https://doi.org/10.1021/ac900415s

APA

Ganser, A., Roth, G., van Galen, J. C., Hilderink, J., Wammes, J. J. G., Müller, I., van Leeuwen, F. N., Wiesmüller, K-H., & Brock, R. (2009). Diffusion-driven device for a high-resolution dose-response profiling of combination chemotherapy. ANAL CHEM, 81(13), 5233-40. https://doi.org/10.1021/ac900415s

Vancouver

Ganser A, Roth G, van Galen JC, Hilderink J, Wammes JJG, Müller I et al. Diffusion-driven device for a high-resolution dose-response profiling of combination chemotherapy. ANAL CHEM. 2009 Jul 1;81(13):5233-40. https://doi.org/10.1021/ac900415s

Bibtex

@article{1d7feaa134ca4aae9a4d69a7a274e267,
title = "Diffusion-driven device for a high-resolution dose-response profiling of combination chemotherapy",
abstract = "Combination therapies have proven vital in the fight against HIV and cancer. However, the identification and optimization of such combination therapies is largely experience driven and an activity of clinicians rather than of systematic screening efforts. Here we present a diffusion device, compatible with the format of a 12-well microtiter plate, to create and test all possible mixtures of two substances with only two pipetting steps. Applications to the testing of different drug combinations and the parallel screening of different leukemia cell lines as well as primary patient cells are presented. The diffusion device yields qualitatively and quantitatively comparable results to an MTT viability assay conducted in a standard 96-well format albeit with a tremendous reduction of processing steps. In addition, a fluorescence-based annexin V binding assay of cell death was implemented. Next to the reduction of processing steps, the diffusion device constitutes a considerable assay miniaturization that overcomes the problems typically associated with miniaturization as a consequence of small sample volumes. Given its ease of handling, the device will greatly advance the development and optimization of combination drugs and the identification of optimum drug combinations in personalized medicine.",
keywords = "Adenine Nucleotides, Annexin A5, Antineoplastic Combined Chemotherapy Protocols, Arabinonucleosides, Cell Death, Diffusion, Dose-Response Relationship, Drug, Doxorubicin, Drug Therapy, Combination, Fluorescent Dyes, Humans, Miniaturization, Phosphatidylserines, Precursor Cell Lymphoblastic Leukemia-Lymphoma, Toxicity Tests, Tumor Cells, Cultured",
author = "Alexander Ganser and G{\"u}nter Roth and {van Galen}, {Joost C} and Janneke Hilderink and Wammes, {Joost J G} and Ingo M{\"u}ller and {van Leeuwen}, {Frank N} and Karl-Heinz Wiesm{\"u}ller and Roland Brock",
year = "2009",
month = jul,
day = "1",
doi = "10.1021/ac900415s",
language = "English",
volume = "81",
pages = "5233--40",
journal = "ANAL CHEM",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "13",

}

RIS

TY - JOUR

T1 - Diffusion-driven device for a high-resolution dose-response profiling of combination chemotherapy

AU - Ganser, Alexander

AU - Roth, Günter

AU - van Galen, Joost C

AU - Hilderink, Janneke

AU - Wammes, Joost J G

AU - Müller, Ingo

AU - van Leeuwen, Frank N

AU - Wiesmüller, Karl-Heinz

AU - Brock, Roland

PY - 2009/7/1

Y1 - 2009/7/1

N2 - Combination therapies have proven vital in the fight against HIV and cancer. However, the identification and optimization of such combination therapies is largely experience driven and an activity of clinicians rather than of systematic screening efforts. Here we present a diffusion device, compatible with the format of a 12-well microtiter plate, to create and test all possible mixtures of two substances with only two pipetting steps. Applications to the testing of different drug combinations and the parallel screening of different leukemia cell lines as well as primary patient cells are presented. The diffusion device yields qualitatively and quantitatively comparable results to an MTT viability assay conducted in a standard 96-well format albeit with a tremendous reduction of processing steps. In addition, a fluorescence-based annexin V binding assay of cell death was implemented. Next to the reduction of processing steps, the diffusion device constitutes a considerable assay miniaturization that overcomes the problems typically associated with miniaturization as a consequence of small sample volumes. Given its ease of handling, the device will greatly advance the development and optimization of combination drugs and the identification of optimum drug combinations in personalized medicine.

AB - Combination therapies have proven vital in the fight against HIV and cancer. However, the identification and optimization of such combination therapies is largely experience driven and an activity of clinicians rather than of systematic screening efforts. Here we present a diffusion device, compatible with the format of a 12-well microtiter plate, to create and test all possible mixtures of two substances with only two pipetting steps. Applications to the testing of different drug combinations and the parallel screening of different leukemia cell lines as well as primary patient cells are presented. The diffusion device yields qualitatively and quantitatively comparable results to an MTT viability assay conducted in a standard 96-well format albeit with a tremendous reduction of processing steps. In addition, a fluorescence-based annexin V binding assay of cell death was implemented. Next to the reduction of processing steps, the diffusion device constitutes a considerable assay miniaturization that overcomes the problems typically associated with miniaturization as a consequence of small sample volumes. Given its ease of handling, the device will greatly advance the development and optimization of combination drugs and the identification of optimum drug combinations in personalized medicine.

KW - Adenine Nucleotides

KW - Annexin A5

KW - Antineoplastic Combined Chemotherapy Protocols

KW - Arabinonucleosides

KW - Cell Death

KW - Diffusion

KW - Dose-Response Relationship, Drug

KW - Doxorubicin

KW - Drug Therapy, Combination

KW - Fluorescent Dyes

KW - Humans

KW - Miniaturization

KW - Phosphatidylserines

KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma

KW - Toxicity Tests

KW - Tumor Cells, Cultured

U2 - 10.1021/ac900415s

DO - 10.1021/ac900415s

M3 - SCORING: Journal article

C2 - 19476343

VL - 81

SP - 5233

EP - 5240

JO - ANAL CHEM

JF - ANAL CHEM

SN - 0003-2700

IS - 13

ER -