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Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage

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Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage. / Shirahashi, Hitoshi; Wu, Jian; Yamamoto, Naoki; Catana, Andreea; Wege, Henning; Wager, Brook; Okita, Kiwamu; Zern, Mark A.

In: CELL TRANSPLANT, Vol. 13, No. 3, 01.01.2004, p. 197-211.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Shirahashi, H, Wu, J, Yamamoto, N, Catana, A, Wege, H, Wager, B, Okita, K & Zern, MA 2004, 'Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage', CELL TRANSPLANT, vol. 13, no. 3, pp. 197-211.

APA

Shirahashi, H., Wu, J., Yamamoto, N., Catana, A., Wege, H., Wager, B., Okita, K., & Zern, M. A. (2004). Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage. CELL TRANSPLANT, 13(3), 197-211.

Vancouver

Shirahashi H, Wu J, Yamamoto N, Catana A, Wege H, Wager B et al. Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage. CELL TRANSPLANT. 2004 Jan 1;13(3):197-211.

Bibtex

@article{baf1b4c88c36401b818db97c73b1f66a,
title = "Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage",
abstract = "Embryonic stem (ES) cells may differentiate along a hepatocyte lineage; however, currently there are no reports of culture conditions yielding high levels of hepatocyte-specific gene expression in these cells. We investigated culture conditions for differentiating ES cells into hepatocyte-like cells in vitro. Various combinations of culture media, growth and differentiation factors, and substratum precoatings were evaluated, and it was determined that a combination of Iscove's modified Dulbecco's medium with 20% fetal bovine serum, human insulin, dexamethasone. and collagen type I precoating was optimal for directing mouse ES cells along a hepatocyte lineage. Treatment of mouse ES cell with the optimal condition led to prealbumin gene expression 20% as high, and albumin synthesis 7% as high, as in mouse liver. The optimal culture condition also induced albumin gene expression in differentiated human ES cells 1% as high as in normal human hepatocytes as shown by Western blot analysis, and cells were positive for human albumin by immunocytochemistry. In addition, our optimal condition led to high levels of albumin gene expression in primary mouse hepatocytes after 35 days of culture, levels 10-fold higher than with other hepatocyte differentiation media. In conclusion, our optimal condition directed both mouse and human ES cells along a hepatocyte lineage. This represents the initial step in establishing cell lines that can be employed in cell-based therapeutics in humans and for toxicology and pharmacology studies.",
keywords = "Albumins, Animals, Blotting, Western, Cell Culture Techniques, Cell Differentiation, Cell Lineage, Cell Transplantation, Culture Media, Dexamethasone, Embryo, Mammalian, Hepatocytes, Humans, Immunohistochemistry, Insulin, Mice, Models, Biological, Species Specificity, Stem Cells, Time Factors, Urea",
author = "Hitoshi Shirahashi and Jian Wu and Naoki Yamamoto and Andreea Catana and Henning Wege and Brook Wager and Kiwamu Okita and Zern, {Mark A}",
year = "2004",
month = jan,
day = "1",
language = "English",
volume = "13",
pages = "197--211",
journal = "CELL TRANSPLANT",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "3",

}

RIS

TY - JOUR

T1 - Differentiation of human and mouse embryonic stem cells along a hepatocyte lineage

AU - Shirahashi, Hitoshi

AU - Wu, Jian

AU - Yamamoto, Naoki

AU - Catana, Andreea

AU - Wege, Henning

AU - Wager, Brook

AU - Okita, Kiwamu

AU - Zern, Mark A

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Embryonic stem (ES) cells may differentiate along a hepatocyte lineage; however, currently there are no reports of culture conditions yielding high levels of hepatocyte-specific gene expression in these cells. We investigated culture conditions for differentiating ES cells into hepatocyte-like cells in vitro. Various combinations of culture media, growth and differentiation factors, and substratum precoatings were evaluated, and it was determined that a combination of Iscove's modified Dulbecco's medium with 20% fetal bovine serum, human insulin, dexamethasone. and collagen type I precoating was optimal for directing mouse ES cells along a hepatocyte lineage. Treatment of mouse ES cell with the optimal condition led to prealbumin gene expression 20% as high, and albumin synthesis 7% as high, as in mouse liver. The optimal culture condition also induced albumin gene expression in differentiated human ES cells 1% as high as in normal human hepatocytes as shown by Western blot analysis, and cells were positive for human albumin by immunocytochemistry. In addition, our optimal condition led to high levels of albumin gene expression in primary mouse hepatocytes after 35 days of culture, levels 10-fold higher than with other hepatocyte differentiation media. In conclusion, our optimal condition directed both mouse and human ES cells along a hepatocyte lineage. This represents the initial step in establishing cell lines that can be employed in cell-based therapeutics in humans and for toxicology and pharmacology studies.

AB - Embryonic stem (ES) cells may differentiate along a hepatocyte lineage; however, currently there are no reports of culture conditions yielding high levels of hepatocyte-specific gene expression in these cells. We investigated culture conditions for differentiating ES cells into hepatocyte-like cells in vitro. Various combinations of culture media, growth and differentiation factors, and substratum precoatings were evaluated, and it was determined that a combination of Iscove's modified Dulbecco's medium with 20% fetal bovine serum, human insulin, dexamethasone. and collagen type I precoating was optimal for directing mouse ES cells along a hepatocyte lineage. Treatment of mouse ES cell with the optimal condition led to prealbumin gene expression 20% as high, and albumin synthesis 7% as high, as in mouse liver. The optimal culture condition also induced albumin gene expression in differentiated human ES cells 1% as high as in normal human hepatocytes as shown by Western blot analysis, and cells were positive for human albumin by immunocytochemistry. In addition, our optimal condition led to high levels of albumin gene expression in primary mouse hepatocytes after 35 days of culture, levels 10-fold higher than with other hepatocyte differentiation media. In conclusion, our optimal condition directed both mouse and human ES cells along a hepatocyte lineage. This represents the initial step in establishing cell lines that can be employed in cell-based therapeutics in humans and for toxicology and pharmacology studies.

KW - Albumins

KW - Animals

KW - Blotting, Western

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cell Lineage

KW - Cell Transplantation

KW - Culture Media

KW - Dexamethasone

KW - Embryo, Mammalian

KW - Hepatocytes

KW - Humans

KW - Immunohistochemistry

KW - Insulin

KW - Mice

KW - Models, Biological

KW - Species Specificity

KW - Stem Cells

KW - Time Factors

KW - Urea

M3 - SCORING: Journal article

C2 - 15191158

VL - 13

SP - 197

EP - 211

JO - CELL TRANSPLANT

JF - CELL TRANSPLANT

SN - 0963-6897

IS - 3

ER -