Research ExplorerPublicationsDifferentiation and development of human female germ cells d...

Differentiation and development of human female germ cells during prenatal gonadogenesis

Standard

Differentiation and development of human female germ cells during prenatal gonadogenesis : an immunohistochemical study. / Stoop, H; Honecker, F; Cools, M; de Krijger, R; Bokemeyer, C; Looijenga, L H J.

In: HUM REPROD, Vol. 20, No. 6, 06.2005, p. 1466-76.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Stoop, H, Honecker, F, Cools, M, de Krijger, R, Bokemeyer, C & Looijenga, LHJ 2005, 'Differentiation and development of human female germ cells during prenatal gonadogenesis: an immunohistochemical study', HUM REPROD, vol. 20, no. 6, pp. 1466-76. https://doi.org/10.1093/humrep/deh800

APA

Stoop, H., Honecker, F., Cools, M., de Krijger, R., Bokemeyer, C., & Looijenga, L. H. J. (2005). Differentiation and development of human female germ cells during prenatal gonadogenesis: an immunohistochemical study. HUM REPROD, 20(6), 1466-76. https://doi.org/10.1093/humrep/deh800

Vancouver

Stoop H, Honecker F, Cools M, de Krijger R, Bokemeyer C, Looijenga LHJ. Differentiation and development of human female germ cells during prenatal gonadogenesis: an immunohistochemical study. HUM REPROD. 2005 Jun;20(6):1466-76. https://doi.org/10.1093/humrep/deh800

Bibtex

@article{63361cbd9ade475d8bd081bc10f60d3e,
title = "Differentiation and development of human female germ cells during prenatal gonadogenesis: an immunohistochemical study",
abstract = "BACKGROUND: In the development of the human ovary, the second trimester includes the transition from oogonial replication to primordial follicle formation. The present study was carried out to assess differentiation and proliferation of germ cells in a series of female gonads from 19 fetuses from the second and third trimester, and two neonates.METHODS: Using immunohistochemistry, the following markers were studied: placental/germ-like cell alkaline phosphatases (PLAP), the marker of pluripotency OCT3/4, the proliferation marker Ki-67, beta-catenin and E-cadherin, the stem cell factor receptor c-KIT, and VASA, a protein specific for the germ cell lineage.RESULTS: PLAP and OCT3/4 were seen during oogenesis, but not in germ cells engaged in folliculogenesis. A similar pattern was observed for Ki-67. Loss of pluripotency occurs once oocytes engage in follicle formation, suggesting a role of cell-cell interactions in the process of germ cell maturation. VASA, c-KIT, beta-catenin and E-cadherin were found in germ cells at all developmental stages of oogenesis and folliculogenesis.CONCLUSIONS: Immunohistochemically, two groups of germ cells can be distinguished. Germ cells that are predominantly found in the cortical region of the ovary before weeks 22-24 of gestation, showing an immature phenotype, are mitotically active, and express OCT3/4, a marker of pluripotency. On the other hand, germ cells undergoing folliculogenesis have lost their pluripotent potential and no longer proliferate.",
keywords = "Alkaline Phosphatase, Biological Markers, Cadherins, Cell Differentiation, Cytoskeletal Proteins, DEAD-box RNA Helicases, DNA-Binding Proteins, Female, Humans, Immunohistochemistry, Ki-67 Antigen, Octamer Transcription Factor-3, Organic Cation Transport Proteins, Ovary, Ovum, Pregnancy, Pregnancy Trimester, Second, Pregnancy Trimester, Third, Proto-Oncogene Proteins c-kit, RNA Helicases, Trans-Activators, Transcription Factors, beta Catenin",
author = "H Stoop and F Honecker and M Cools and {de Krijger}, R and C Bokemeyer and Looijenga, {L H J}",
year = "2005",
month = jun,
doi = "10.1093/humrep/deh800",
language = "English",
volume = "20",
pages = "1466--76",
journal = "HUM REPROD",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Differentiation and development of human female germ cells during prenatal gonadogenesis

T2 - an immunohistochemical study

AU - Stoop, H

AU - Honecker, F

AU - Cools, M

AU - de Krijger, R

AU - Bokemeyer, C

AU - Looijenga, L H J

PY - 2005/6

Y1 - 2005/6

N2 - BACKGROUND: In the development of the human ovary, the second trimester includes the transition from oogonial replication to primordial follicle formation. The present study was carried out to assess differentiation and proliferation of germ cells in a series of female gonads from 19 fetuses from the second and third trimester, and two neonates.METHODS: Using immunohistochemistry, the following markers were studied: placental/germ-like cell alkaline phosphatases (PLAP), the marker of pluripotency OCT3/4, the proliferation marker Ki-67, beta-catenin and E-cadherin, the stem cell factor receptor c-KIT, and VASA, a protein specific for the germ cell lineage.RESULTS: PLAP and OCT3/4 were seen during oogenesis, but not in germ cells engaged in folliculogenesis. A similar pattern was observed for Ki-67. Loss of pluripotency occurs once oocytes engage in follicle formation, suggesting a role of cell-cell interactions in the process of germ cell maturation. VASA, c-KIT, beta-catenin and E-cadherin were found in germ cells at all developmental stages of oogenesis and folliculogenesis.CONCLUSIONS: Immunohistochemically, two groups of germ cells can be distinguished. Germ cells that are predominantly found in the cortical region of the ovary before weeks 22-24 of gestation, showing an immature phenotype, are mitotically active, and express OCT3/4, a marker of pluripotency. On the other hand, germ cells undergoing folliculogenesis have lost their pluripotent potential and no longer proliferate.

AB - BACKGROUND: In the development of the human ovary, the second trimester includes the transition from oogonial replication to primordial follicle formation. The present study was carried out to assess differentiation and proliferation of germ cells in a series of female gonads from 19 fetuses from the second and third trimester, and two neonates.METHODS: Using immunohistochemistry, the following markers were studied: placental/germ-like cell alkaline phosphatases (PLAP), the marker of pluripotency OCT3/4, the proliferation marker Ki-67, beta-catenin and E-cadherin, the stem cell factor receptor c-KIT, and VASA, a protein specific for the germ cell lineage.RESULTS: PLAP and OCT3/4 were seen during oogenesis, but not in germ cells engaged in folliculogenesis. A similar pattern was observed for Ki-67. Loss of pluripotency occurs once oocytes engage in follicle formation, suggesting a role of cell-cell interactions in the process of germ cell maturation. VASA, c-KIT, beta-catenin and E-cadherin were found in germ cells at all developmental stages of oogenesis and folliculogenesis.CONCLUSIONS: Immunohistochemically, two groups of germ cells can be distinguished. Germ cells that are predominantly found in the cortical region of the ovary before weeks 22-24 of gestation, showing an immature phenotype, are mitotically active, and express OCT3/4, a marker of pluripotency. On the other hand, germ cells undergoing folliculogenesis have lost their pluripotent potential and no longer proliferate.

KW - Alkaline Phosphatase

KW - Biological Markers

KW - Cadherins

KW - Cell Differentiation

KW - Cytoskeletal Proteins

KW - DEAD-box RNA Helicases

KW - DNA-Binding Proteins

KW - Female

KW - Humans

KW - Immunohistochemistry

KW - Ki-67 Antigen

KW - Octamer Transcription Factor-3

KW - Organic Cation Transport Proteins

KW - Ovary

KW - Ovum

KW - Pregnancy

KW - Pregnancy Trimester, Second

KW - Pregnancy Trimester, Third

KW - Proto-Oncogene Proteins c-kit

KW - RNA Helicases

KW - Trans-Activators

KW - Transcription Factors

KW - beta Catenin

U2 - 10.1093/humrep/deh800

DO - 10.1093/humrep/deh800

M3 - SCORING: Journal article

C2 - 15734757

VL - 20

SP - 1466

EP - 1476

JO - HUM REPROD

JF - HUM REPROD

SN - 0268-1161

IS - 6

ER -