Differential distribution of endoplasmic reticulum controls metabotropic signaling and plasticity at hippocampal synapses.
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Differential distribution of endoplasmic reticulum controls metabotropic signaling and plasticity at hippocampal synapses. / Holbro, Niklaus; Grunditz, Asa; Oertner, Thomas G.
In: P NATL ACAD SCI USA, Vol. 106, No. 35, 35, 2009, p. 15055-15060.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Differential distribution of endoplasmic reticulum controls metabotropic signaling and plasticity at hippocampal synapses.
AU - Holbro, Niklaus
AU - Grunditz, Asa
AU - Oertner, Thomas G.
PY - 2009
Y1 - 2009
N2 - Synaptic plasticity is considered essential for learning and storage of new memories. Whether all synapses on a given neuron have the same ability to express long-term plasticity is not well understood. Synaptic microanatomy could affect the function of local signaling cascades and thus differentially regulate the potential for plasticity at individual synapses. Here, we investigate how the presence of endoplasmic reticulum (ER) in dendritic spines of CA1 pyramidal neurons affects postsynaptic signaling. We show that the ER is targeted selectively to large spines containing strong synapses. In ER-containing spines, we frequently observed synaptically triggered calcium release events of very large amplitudes. Low-frequency stimulation of these spines induced a permanent depression of synaptic potency that was independent of NMDA receptor activation and specific to the stimulated synapses. In contrast, no functional changes were induced in the majority of spines lacking ER. Both calcium release events and long-term depression depended on the activation of metabotropic glutamate receptors and inositol trisphosphate receptors. In summary, spine microanatomy is a reliable indicator for the presence of specific signaling cascades that govern plasticity on a micrometer scale.
AB - Synaptic plasticity is considered essential for learning and storage of new memories. Whether all synapses on a given neuron have the same ability to express long-term plasticity is not well understood. Synaptic microanatomy could affect the function of local signaling cascades and thus differentially regulate the potential for plasticity at individual synapses. Here, we investigate how the presence of endoplasmic reticulum (ER) in dendritic spines of CA1 pyramidal neurons affects postsynaptic signaling. We show that the ER is targeted selectively to large spines containing strong synapses. In ER-containing spines, we frequently observed synaptically triggered calcium release events of very large amplitudes. Low-frequency stimulation of these spines induced a permanent depression of synaptic potency that was independent of NMDA receptor activation and specific to the stimulated synapses. In contrast, no functional changes were induced in the majority of spines lacking ER. Both calcium release events and long-term depression depended on the activation of metabotropic glutamate receptors and inositol trisphosphate receptors. In summary, spine microanatomy is a reliable indicator for the presence of specific signaling cascades that govern plasticity on a micrometer scale.
KW - Animals
KW - Rats
KW - Rats, Wistar
KW - Calcium/metabolism
KW - Endoplasmic Reticulum/metabolism
KW - Hippocampus/cytology/metabolism
KW - Neuronal Plasticity
KW - Receptors, Metabotropic Glutamate/metabolism
KW - Receptors, N-Methyl-D-Aspartate/metabolism
KW - Signal Transduction
KW - Synapses/metabolism
KW - Tissue Culture Techniques
KW - Animals
KW - Rats
KW - Rats, Wistar
KW - Calcium/metabolism
KW - Endoplasmic Reticulum/metabolism
KW - Hippocampus/cytology/metabolism
KW - Neuronal Plasticity
KW - Receptors, Metabotropic Glutamate/metabolism
KW - Receptors, N-Methyl-D-Aspartate/metabolism
KW - Signal Transduction
KW - Synapses/metabolism
KW - Tissue Culture Techniques
M3 - SCORING: Journal article
VL - 106
SP - 15055
EP - 15060
JO - P NATL ACAD SCI USA
JF - P NATL ACAD SCI USA
SN - 0027-8424
IS - 35
M1 - 35
ER -