Differente Integrin-vermittelte Adhäsion von hoch hepatisch metastasierenden und gering metastasierenden Kolon-Karzinom-Zellen an extrazellulärer Matrix
Standard
Differente Integrin-vermittelte Adhäsion von hoch hepatisch metastasierenden und gering metastasierenden Kolon-Karzinom-Zellen an extrazellulärer Matrix. / Haier, J; Nasralla, M; Buhr, H J; Nicolson, G L.
In: Langenbecks Arch Chir Suppl Kongressbd, Vol. 115, No. Suppl I, 1998, p. 307-13.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Differente Integrin-vermittelte Adhäsion von hoch hepatisch metastasierenden und gering metastasierenden Kolon-Karzinom-Zellen an extrazellulärer Matrix
AU - Haier, J
AU - Nasralla, M
AU - Buhr, H J
AU - Nicolson, G L
PY - 1998
Y1 - 1998
N2 - Poorly and highly liver metastatic colon carcinoma cell lines have different integrin-mediated adhesion to extracellular matrix. Specific integrin-mediated interactions between tumor cells and extracellular matrix (ECM) in the host organs are important for organ-specific metastasis. In colon carcinoma integrin expression differs depending on the metastatic potential of the tumor. Integrin-mediated adhesion of poorly (HT-29P) and highly liver-metastatic (HT-29LMM) colon carcinoma to extracellular matrix (ECM; Collagen I-C I, Collagen IV-C IV, Laminin LN, Fibronectin FN, Vitronectin VN) was investigated. HT-29LMM showed significant better adhesion to LN (45% vs. 26%; p < 0.001) and FN 20% vs. 1%; p < 0.001). No adhesion was found to VN. RGD-oligopeptides completely inhibited adhesion to FN. Using inhibition with anti-integrin-mAB it was shown, that adhesion to C I and C IV is mediated by alpha 2 beta 1-integrin, adhesion to LN by alpha 6 beta 1 and adhesion to FN by alpha v beta 1. These results have shown that adhesion of HT-29 cells is mediated by different integrins depending on ECM components. Poorly and highly metastatic cells possessed different patterns of adhesion to various substrates.
AB - Poorly and highly liver metastatic colon carcinoma cell lines have different integrin-mediated adhesion to extracellular matrix. Specific integrin-mediated interactions between tumor cells and extracellular matrix (ECM) in the host organs are important for organ-specific metastasis. In colon carcinoma integrin expression differs depending on the metastatic potential of the tumor. Integrin-mediated adhesion of poorly (HT-29P) and highly liver-metastatic (HT-29LMM) colon carcinoma to extracellular matrix (ECM; Collagen I-C I, Collagen IV-C IV, Laminin LN, Fibronectin FN, Vitronectin VN) was investigated. HT-29LMM showed significant better adhesion to LN (45% vs. 26%; p < 0.001) and FN 20% vs. 1%; p < 0.001). No adhesion was found to VN. RGD-oligopeptides completely inhibited adhesion to FN. Using inhibition with anti-integrin-mAB it was shown, that adhesion to C I and C IV is mediated by alpha 2 beta 1-integrin, adhesion to LN by alpha 6 beta 1 and adhesion to FN by alpha v beta 1. These results have shown that adhesion of HT-29 cells is mediated by different integrins depending on ECM components. Poorly and highly metastatic cells possessed different patterns of adhesion to various substrates.
KW - Cell Adhesion
KW - Colonic Neoplasms
KW - Extracellular Matrix
KW - Gene Expression Regulation, Neoplastic
KW - Humans
KW - Integrins
KW - Liver
KW - Liver Neoplasms
KW - Prognosis
KW - Tumor Cells, Cultured
M3 - SCORING: Zeitschriftenaufsatz
C2 - 14518266
VL - 115
SP - 307
EP - 313
IS - Suppl I
ER -
