[Diagnostics of acute leukemias: interaction of phenotypic and genetic methods].
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[Diagnostics of acute leukemias: interaction of phenotypic and genetic methods]. / Bacher, Ulrike; Haferlach, C; Schnittger, S; Kern, W; Ott, M M; Haferlach, T.
In: PATHOLOGE, Vol. 33, No. 6, 6, 2012, p. 528-538.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - [Diagnostics of acute leukemias: interaction of phenotypic and genetic methods].
AU - Bacher, Ulrike
AU - Haferlach, C
AU - Schnittger, S
AU - Kern, W
AU - Ott, M M
AU - Haferlach, T
PY - 2012
Y1 - 2012
N2 - Due to the heterogeneity of these disorders, the diagnosis of acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) requires a broad spectrum of laboratory techniques: cytomorphology, immunophenotyping, chromosome banding analysis, fluorescence in situ hybridization, and molecular genetics. The cytomorphological leukemia subtypes can be indicative for distinct genetic alterations and contribute to the guidance of the further diagnostic process. Immunophenotyping allows to define the hematological lineage and to characterize the leukemia-associated immunophenotype as basis for follow up investigation. Cytogenetic alterations and molecular mutations are essential for the correct classification of cases and for prognostication. Molecular markers are helpful to define the minimal residual disease load after the achievement of hematological complete remission. In cases of hypocellular AML or in case of bone marrow necrosis, histopathology in combination with immunohistochemistry is of importance. Hierarchies between the different techniques catalyze the workflow in the laboratory and allow a rapid diagnosis and classification of the leukemia cases.
AB - Due to the heterogeneity of these disorders, the diagnosis of acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) requires a broad spectrum of laboratory techniques: cytomorphology, immunophenotyping, chromosome banding analysis, fluorescence in situ hybridization, and molecular genetics. The cytomorphological leukemia subtypes can be indicative for distinct genetic alterations and contribute to the guidance of the further diagnostic process. Immunophenotyping allows to define the hematological lineage and to characterize the leukemia-associated immunophenotype as basis for follow up investigation. Cytogenetic alterations and molecular mutations are essential for the correct classification of cases and for prognostication. Molecular markers are helpful to define the minimal residual disease load after the achievement of hematological complete remission. In cases of hypocellular AML or in case of bone marrow necrosis, histopathology in combination with immunohistochemistry is of importance. Hierarchies between the different techniques catalyze the workflow in the laboratory and allow a rapid diagnosis and classification of the leukemia cases.
KW - Humans
KW - Immunohistochemistry
KW - Prognosis
KW - Immunophenotyping
KW - In Situ Hybridization, Fluorescence
KW - Phenotype
KW - Cytogenetic Analysis
KW - Chromosome Banding
KW - Necrosis
KW - Genetic Markers/genetics
KW - Bone Marrow/pathology
KW - Leukemia, Myeloid, Acute/classification/diagnosis/genetics/pathology
KW - Pathology, Molecular
KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma/classification/diagnosis/genetics/pathology
KW - Workflow
KW - Humans
KW - Immunohistochemistry
KW - Prognosis
KW - Immunophenotyping
KW - In Situ Hybridization, Fluorescence
KW - Phenotype
KW - Cytogenetic Analysis
KW - Chromosome Banding
KW - Necrosis
KW - Genetic Markers/genetics
KW - Bone Marrow/pathology
KW - Leukemia, Myeloid, Acute/classification/diagnosis/genetics/pathology
KW - Pathology, Molecular
KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma/classification/diagnosis/genetics/pathology
KW - Workflow
M3 - SCORING: Zeitschriftenaufsatz
VL - 33
SP - 528
EP - 538
JO - PATHOLOGE
JF - PATHOLOGE
SN - 0172-8113
IS - 6
M1 - 6
ER -
