Research ExplorerPublicationsDiagnostic performance of capillary and venous blood samples...

Diagnostic performance of capillary and venous blood samples in the detection of Loa loa and Mansonella perstans microfilaraemia using light microscopy

Standard

Diagnostic performance of capillary and venous blood samples in the detection of Loa loa and Mansonella perstans microfilaraemia using light microscopy. / Mischlinger, Johannes; Manego, Rella Zoleko; Mombo-Ngoma, Ghyslain; Ekoka Mbassi, Dorothea; Hackbarth, Nina; Ekoka Mbassi, Franck-Aurelien; Davi, Saskia Dede; Kreuzmair, Ruth; Veletzky, Luzia; Hergeth, Jennifer; Ndoumba, Wilfrid Nzebe; Pitzinger, Paul; Groger, Mirjam; Matsiegui, Pierre Blaise; Adegnika, Ayôla Akim; Agnandji, Selidji Todagbe; Lell, Bertrand; Ramharter, Michael.

In: PLOS NEGLECT TROP D, Vol. 15, No. 8, e0009623, 08.2021.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mischlinger, J, Manego, RZ, Mombo-Ngoma, G, Ekoka Mbassi, D, Hackbarth, N, Ekoka Mbassi, F-A, Davi, SD, Kreuzmair, R, Veletzky, L, Hergeth, J, Ndoumba, WN, Pitzinger, P, Groger, M, Matsiegui, PB, Adegnika, AA, Agnandji, ST, Lell, B & Ramharter, M 2021, 'Diagnostic performance of capillary and venous blood samples in the detection of Loa loa and Mansonella perstans microfilaraemia using light microscopy', PLOS NEGLECT TROP D, vol. 15, no. 8, e0009623. https://doi.org/10.1371/journal.pntd.0009623

APA

Mischlinger, J., Manego, R. Z., Mombo-Ngoma, G., Ekoka Mbassi, D., Hackbarth, N., Ekoka Mbassi, F-A., Davi, S. D., Kreuzmair, R., Veletzky, L., Hergeth, J., Ndoumba, W. N., Pitzinger, P., Groger, M., Matsiegui, P. B., Adegnika, A. A., Agnandji, S. T., Lell, B., & Ramharter, M. (2021). Diagnostic performance of capillary and venous blood samples in the detection of Loa loa and Mansonella perstans microfilaraemia using light microscopy. PLOS NEGLECT TROP D, 15(8), [e0009623]. https://doi.org/10.1371/journal.pntd.0009623

Vancouver

Mischlinger J, Manego RZ, Mombo-Ngoma G, Ekoka Mbassi D, Hackbarth N, Ekoka Mbassi F-A et al. Diagnostic performance of capillary and venous blood samples in the detection of Loa loa and Mansonella perstans microfilaraemia using light microscopy. PLOS NEGLECT TROP D. 2021 Aug;15(8). e0009623. https://doi.org/10.1371/journal.pntd.0009623

Bibtex

@article{b5a2d94c04f643d0a1c9d7cace6023ad,
title = "Diagnostic performance of capillary and venous blood samples in the detection of Loa loa and Mansonella perstans microfilaraemia using light microscopy",
abstract = "BACKGROUND: Loa loa and Mansonella perstans-the causative agents of loiasis and mansonellosis-are vector-borne filarial parasites co-endemic in sub-Saharan Africa. Diagnosis of both infections is usually established by microscopic analysis of blood samples. It was recently established that the odds for detecting Plasmodium spp. is higher in capillary (CAP) blood than in venous (VEN) blood. In analogy to this finding this analysis evaluates potential differences in microfilaraemia of L. loa and M. perstans in samples of CAP and VEN blood.METHODS: Recruitment took place between 2015 and 2019 at the CERMEL in Lambar{\'e}n{\'e}, Gabon and its surrounding villages. Persons of all ages presenting to diagnostic services of the research center around noon were invited to participate in the study. A thick smear of each 10 microliters of CAP and VEN blood was prepared and analysed by a minimum of two independent microscopists. Differences of log2-transformed CAP and VEN microfilaraemia were computed and expressed as percentages. Furthermore, odds ratios for paired data were computed to quantify the odds to detect microfilariae in CAP blood versus in VEN blood.RESULTS: A total of 713 participants were recruited among whom 52% were below 30 years of age, 27% between 30-59 years of age and 21% above 60 years of age. Male-female ratio was 0.84. Among 152 participants with microscopically-confirmed L. loa infection median (IQR) microfilaraemia was 3,650 (275-11,100) per milliliter blood in CAP blood and 2,775 (200-8,875) in VEN blood (p<0.0001), while among 102 participants with M. perstans this was 100 (0-200) and 100 (0-200), respectively (p = 0.44). Differences in linear models amount up to an average of +34.5% (95% CI: +11.0 to +63.0) higher L. loa microfilaria quantity in CAP blood versus VEN blood and for M. perstans it was on average higher by +24.8% (95% CI: +0.0 to +60.5). Concordantly, the odds for detection of microfilaraemia in CAP samples versus VEN samples was 1.24 (95% CI: 0.65-2.34) and 1.65 (95% CI: 1.0-2.68) for infections with L. loa and M. perstans, respectively.CONCLUSION: This analysis indicates that average levels of microfilaraemia of L. loa are higher in CAP blood samples than in VEN blood samples. This might have implications for treatment algorithms of onchocerciasis and loiasis, in which exact quantification of L. loa microfilaraemia is of importance. Furthermore, the odds for detection of M. perstans microfilariae was higher in CAP than in VEN blood which may pre-dispose CAP blood for detection of M. perstans infection in large epidemiological studies when sampling of large blood quantities is not feasible. No solid evidence for a higher odds of L. loa microfilariae detection in CAP blood was revealed, which might be explained by generally high levels of L. loa microfilaraemia in CAP and VEN blood above the limit of detection of 100 microfilariae/ml. Yet, it cannot be excluded that the study was underpowered to detect a moderate difference.",
keywords = "Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Coinfection/epidemiology, Female, Gabon/epidemiology, Humans, Loa/isolation & purification, Loiasis/epidemiology, Male, Mansonella/isolation & purification, Mansonelliasis/epidemiology, Microscopy, Middle Aged, Parasite Load, Parasitemia, Prevalence, Serologic Tests, Young Adult",
author = "Johannes Mischlinger and Manego, {Rella Zoleko} and Ghyslain Mombo-Ngoma and {Ekoka Mbassi}, Dorothea and Nina Hackbarth and {Ekoka Mbassi}, Franck-Aurelien and Davi, {Saskia Dede} and Ruth Kreuzmair and Luzia Veletzky and Jennifer Hergeth and Ndoumba, {Wilfrid Nzebe} and Paul Pitzinger and Mirjam Groger and Matsiegui, {Pierre Blaise} and Adegnika, {Ay{\^o}la Akim} and Agnandji, {Selidji Todagbe} and Bertrand Lell and Michael Ramharter",
year = "2021",
month = aug,
doi = "10.1371/journal.pntd.0009623",
language = "English",
volume = "15",
journal = "PLOS NEGLECT TROP D",
issn = "1935-2735",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - Diagnostic performance of capillary and venous blood samples in the detection of Loa loa and Mansonella perstans microfilaraemia using light microscopy

AU - Mischlinger, Johannes

AU - Manego, Rella Zoleko

AU - Mombo-Ngoma, Ghyslain

AU - Ekoka Mbassi, Dorothea

AU - Hackbarth, Nina

AU - Ekoka Mbassi, Franck-Aurelien

AU - Davi, Saskia Dede

AU - Kreuzmair, Ruth

AU - Veletzky, Luzia

AU - Hergeth, Jennifer

AU - Ndoumba, Wilfrid Nzebe

AU - Pitzinger, Paul

AU - Groger, Mirjam

AU - Matsiegui, Pierre Blaise

AU - Adegnika, Ayôla Akim

AU - Agnandji, Selidji Todagbe

AU - Lell, Bertrand

AU - Ramharter, Michael

PY - 2021/8

Y1 - 2021/8

N2 - BACKGROUND: Loa loa and Mansonella perstans-the causative agents of loiasis and mansonellosis-are vector-borne filarial parasites co-endemic in sub-Saharan Africa. Diagnosis of both infections is usually established by microscopic analysis of blood samples. It was recently established that the odds for detecting Plasmodium spp. is higher in capillary (CAP) blood than in venous (VEN) blood. In analogy to this finding this analysis evaluates potential differences in microfilaraemia of L. loa and M. perstans in samples of CAP and VEN blood.METHODS: Recruitment took place between 2015 and 2019 at the CERMEL in Lambaréné, Gabon and its surrounding villages. Persons of all ages presenting to diagnostic services of the research center around noon were invited to participate in the study. A thick smear of each 10 microliters of CAP and VEN blood was prepared and analysed by a minimum of two independent microscopists. Differences of log2-transformed CAP and VEN microfilaraemia were computed and expressed as percentages. Furthermore, odds ratios for paired data were computed to quantify the odds to detect microfilariae in CAP blood versus in VEN blood.RESULTS: A total of 713 participants were recruited among whom 52% were below 30 years of age, 27% between 30-59 years of age and 21% above 60 years of age. Male-female ratio was 0.84. Among 152 participants with microscopically-confirmed L. loa infection median (IQR) microfilaraemia was 3,650 (275-11,100) per milliliter blood in CAP blood and 2,775 (200-8,875) in VEN blood (p<0.0001), while among 102 participants with M. perstans this was 100 (0-200) and 100 (0-200), respectively (p = 0.44). Differences in linear models amount up to an average of +34.5% (95% CI: +11.0 to +63.0) higher L. loa microfilaria quantity in CAP blood versus VEN blood and for M. perstans it was on average higher by +24.8% (95% CI: +0.0 to +60.5). Concordantly, the odds for detection of microfilaraemia in CAP samples versus VEN samples was 1.24 (95% CI: 0.65-2.34) and 1.65 (95% CI: 1.0-2.68) for infections with L. loa and M. perstans, respectively.CONCLUSION: This analysis indicates that average levels of microfilaraemia of L. loa are higher in CAP blood samples than in VEN blood samples. This might have implications for treatment algorithms of onchocerciasis and loiasis, in which exact quantification of L. loa microfilaraemia is of importance. Furthermore, the odds for detection of M. perstans microfilariae was higher in CAP than in VEN blood which may pre-dispose CAP blood for detection of M. perstans infection in large epidemiological studies when sampling of large blood quantities is not feasible. No solid evidence for a higher odds of L. loa microfilariae detection in CAP blood was revealed, which might be explained by generally high levels of L. loa microfilaraemia in CAP and VEN blood above the limit of detection of 100 microfilariae/ml. Yet, it cannot be excluded that the study was underpowered to detect a moderate difference.

AB - BACKGROUND: Loa loa and Mansonella perstans-the causative agents of loiasis and mansonellosis-are vector-borne filarial parasites co-endemic in sub-Saharan Africa. Diagnosis of both infections is usually established by microscopic analysis of blood samples. It was recently established that the odds for detecting Plasmodium spp. is higher in capillary (CAP) blood than in venous (VEN) blood. In analogy to this finding this analysis evaluates potential differences in microfilaraemia of L. loa and M. perstans in samples of CAP and VEN blood.METHODS: Recruitment took place between 2015 and 2019 at the CERMEL in Lambaréné, Gabon and its surrounding villages. Persons of all ages presenting to diagnostic services of the research center around noon were invited to participate in the study. A thick smear of each 10 microliters of CAP and VEN blood was prepared and analysed by a minimum of two independent microscopists. Differences of log2-transformed CAP and VEN microfilaraemia were computed and expressed as percentages. Furthermore, odds ratios for paired data were computed to quantify the odds to detect microfilariae in CAP blood versus in VEN blood.RESULTS: A total of 713 participants were recruited among whom 52% were below 30 years of age, 27% between 30-59 years of age and 21% above 60 years of age. Male-female ratio was 0.84. Among 152 participants with microscopically-confirmed L. loa infection median (IQR) microfilaraemia was 3,650 (275-11,100) per milliliter blood in CAP blood and 2,775 (200-8,875) in VEN blood (p<0.0001), while among 102 participants with M. perstans this was 100 (0-200) and 100 (0-200), respectively (p = 0.44). Differences in linear models amount up to an average of +34.5% (95% CI: +11.0 to +63.0) higher L. loa microfilaria quantity in CAP blood versus VEN blood and for M. perstans it was on average higher by +24.8% (95% CI: +0.0 to +60.5). Concordantly, the odds for detection of microfilaraemia in CAP samples versus VEN samples was 1.24 (95% CI: 0.65-2.34) and 1.65 (95% CI: 1.0-2.68) for infections with L. loa and M. perstans, respectively.CONCLUSION: This analysis indicates that average levels of microfilaraemia of L. loa are higher in CAP blood samples than in VEN blood samples. This might have implications for treatment algorithms of onchocerciasis and loiasis, in which exact quantification of L. loa microfilaraemia is of importance. Furthermore, the odds for detection of M. perstans microfilariae was higher in CAP than in VEN blood which may pre-dispose CAP blood for detection of M. perstans infection in large epidemiological studies when sampling of large blood quantities is not feasible. No solid evidence for a higher odds of L. loa microfilariae detection in CAP blood was revealed, which might be explained by generally high levels of L. loa microfilaraemia in CAP and VEN blood above the limit of detection of 100 microfilariae/ml. Yet, it cannot be excluded that the study was underpowered to detect a moderate difference.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Animals

KW - Child

KW - Coinfection/epidemiology

KW - Female

KW - Gabon/epidemiology

KW - Humans

KW - Loa/isolation & purification

KW - Loiasis/epidemiology

KW - Male

KW - Mansonella/isolation & purification

KW - Mansonelliasis/epidemiology

KW - Microscopy

KW - Middle Aged

KW - Parasite Load

KW - Parasitemia

KW - Prevalence

KW - Serologic Tests

KW - Young Adult

U2 - 10.1371/journal.pntd.0009623

DO - 10.1371/journal.pntd.0009623

M3 - SCORING: Journal article

C2 - 34398886

VL - 15

JO - PLOS NEGLECT TROP D

JF - PLOS NEGLECT TROP D

SN - 1935-2735

IS - 8

M1 - e0009623

ER -