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Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum

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Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum. / Härter, Georg; Frickmann, Hagen; Zenk, Sebastian; Wichmann, Dominic; Ammann, Bettina; Kern, Peter; Fleischer, Bernhard; Tannich, Egbert; Poppert, Sven.

In: J MED MICROBIOL, Vol. 63, No. Pt 2, 01.02.2014, p. 309-12.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Härter, G, Frickmann, H, Zenk, S, Wichmann, D, Ammann, B, Kern, P, Fleischer, B, Tannich, E & Poppert, S 2014, 'Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum', J MED MICROBIOL, vol. 63, no. Pt 2, pp. 309-12. https://doi.org/10.1099/jmm.0.066142-0

APA

Härter, G., Frickmann, H., Zenk, S., Wichmann, D., Ammann, B., Kern, P., Fleischer, B., Tannich, E., & Poppert, S. (2014). Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum. J MED MICROBIOL, 63(Pt 2), 309-12. https://doi.org/10.1099/jmm.0.066142-0

Vancouver

Härter G, Frickmann H, Zenk S, Wichmann D, Ammann B, Kern P et al. Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum. J MED MICROBIOL. 2014 Feb 1;63(Pt 2):309-12. https://doi.org/10.1099/jmm.0.066142-0

Bibtex

@article{677704ce76b848008f8ae4a167e6f63c,
title = "Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum",
abstract = "We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum-cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.",
keywords = "Adolescent, Animals, Antibodies, Helminth, Antibody Specificity, DNA, Helminth, Emigrants and Immigrants, Germany, Ghana, Humans, Male, Neuroschistosomiasis, Real-Time Polymerase Chain Reaction, Schistosoma",
author = "Georg H{\"a}rter and Hagen Frickmann and Sebastian Zenk and Dominic Wichmann and Bettina Ammann and Peter Kern and Bernhard Fleischer and Egbert Tannich and Sven Poppert",
year = "2014",
month = feb,
day = "1",
doi = "10.1099/jmm.0.066142-0",
language = "English",
volume = "63",
pages = "309--12",
journal = "J MED MICROBIOL",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "Pt 2",

}

RIS

TY - JOUR

T1 - Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum

AU - Härter, Georg

AU - Frickmann, Hagen

AU - Zenk, Sebastian

AU - Wichmann, Dominic

AU - Ammann, Bettina

AU - Kern, Peter

AU - Fleischer, Bernhard

AU - Tannich, Egbert

AU - Poppert, Sven

PY - 2014/2/1

Y1 - 2014/2/1

N2 - We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum-cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.

AB - We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum-cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.

KW - Adolescent

KW - Animals

KW - Antibodies, Helminth

KW - Antibody Specificity

KW - DNA, Helminth

KW - Emigrants and Immigrants

KW - Germany

KW - Ghana

KW - Humans

KW - Male

KW - Neuroschistosomiasis

KW - Real-Time Polymerase Chain Reaction

KW - Schistosoma

U2 - 10.1099/jmm.0.066142-0

DO - 10.1099/jmm.0.066142-0

M3 - SCORING: Journal article

C2 - 24227876

VL - 63

SP - 309

EP - 312

JO - J MED MICROBIOL

JF - J MED MICROBIOL

SN - 0022-2615

IS - Pt 2

ER -