Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation
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Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation. / Wrzeszcz, Antonina; Dittrich, Barbara; Haamann, Daniel; Aliuos, Pooyan; Klee, Doris; Nolte, Ingo; Lenarz, Thomas; Reuter, Günter.
In: J BIOMED MATER RES A, Vol. 102, No. 2, 02.2014, p. 442-54.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Dexamethasone released from cochlear implant coatings combined with a protein repellent hydrogel layer inhibits fibroblast proliferation
AU - Wrzeszcz, Antonina
AU - Dittrich, Barbara
AU - Haamann, Daniel
AU - Aliuos, Pooyan
AU - Klee, Doris
AU - Nolte, Ingo
AU - Lenarz, Thomas
AU - Reuter, Günter
N1 - © 2013 Wiley Periodicals, Inc.
PY - 2014/2
Y1 - 2014/2
N2 - The insertion of cochlear implants into the inner ear often causes inflammation and fibrosis inside the scala tympani and thus growth of fibrous tissue on the implant surface. This deposition leads to the loss of function in both electrical and laser-based implants. The design of this study was to realize fibroblast growth inhibition by dexamethasone (Dex) released from the base material of the implant [polydimethylsiloxane (PDMS)]. To prevent cell and protein adhesion, the PDMS was coated with a hydrogel layer [star-shaped polyethylene glycol prepolymer (sPEG)]. Drug release rates were studied over 3 months, and surface characterization was performed. It was observed that the hydrogel slightly smoothened the surface roughened by the Dex crystals. The hydrogel coating reduced and prolonged the release of the drug over several months. Unmodified, sPEG-coated, Dex-loaded, and Dex/sPEG-equipped PDMS filaments were cocultivated in vitro with fluorescent fibroblasts, analyzed by fluorescent microscopy, and quantified by cell counting. Compared to the unmodified PDMS, cell growth on all modified filaments was averagely 95% ±standard deviation (SD) less, while cell growth on the bottom of the culture dishes containing Dex-loaded filaments was reduced by 70% ±SD. Both, Dex and sPEG prevented direct cell growth on the filament surfaces, while drug delivery was maintained for the duration of several months.
AB - The insertion of cochlear implants into the inner ear often causes inflammation and fibrosis inside the scala tympani and thus growth of fibrous tissue on the implant surface. This deposition leads to the loss of function in both electrical and laser-based implants. The design of this study was to realize fibroblast growth inhibition by dexamethasone (Dex) released from the base material of the implant [polydimethylsiloxane (PDMS)]. To prevent cell and protein adhesion, the PDMS was coated with a hydrogel layer [star-shaped polyethylene glycol prepolymer (sPEG)]. Drug release rates were studied over 3 months, and surface characterization was performed. It was observed that the hydrogel slightly smoothened the surface roughened by the Dex crystals. The hydrogel coating reduced and prolonged the release of the drug over several months. Unmodified, sPEG-coated, Dex-loaded, and Dex/sPEG-equipped PDMS filaments were cocultivated in vitro with fluorescent fibroblasts, analyzed by fluorescent microscopy, and quantified by cell counting. Compared to the unmodified PDMS, cell growth on all modified filaments was averagely 95% ±standard deviation (SD) less, while cell growth on the bottom of the culture dishes containing Dex-loaded filaments was reduced by 70% ±SD. Both, Dex and sPEG prevented direct cell growth on the filament surfaces, while drug delivery was maintained for the duration of several months.
KW - Animals
KW - Anti-Inflammatory Agents
KW - Coated Materials, Biocompatible
KW - Cochlear Implants
KW - Delayed-Action Preparations
KW - Dexamethasone
KW - Dimethylpolysiloxanes
KW - Fibroblasts
KW - Hydrogels
KW - Materials Testing
KW - Mice
KW - Nylons
KW - Time Factors
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1002/jbm.a.34719
DO - 10.1002/jbm.a.34719
M3 - SCORING: Journal article
C2 - 23533184
VL - 102
SP - 442
EP - 454
JO - J BIOMED MATER RES A
JF - J BIOMED MATER RES A
SN - 1549-3296
IS - 2
ER -