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Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex

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Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex. / Schüller, U; Lamp, E C; Schilling, Martin K.

In: HISTOCHEM CELL BIOL, Vol. 116, No. 2, 08.2001, p. 149-59.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schüller, U, Lamp, EC & Schilling, MK 2001, 'Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex', HISTOCHEM CELL BIOL, vol. 116, no. 2, pp. 149-59. https://doi.org/10.1007/s004180100303

APA

Schüller, U., Lamp, E. C., & Schilling, M. K. (2001). Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex. HISTOCHEM CELL BIOL, 116(2), 149-59. https://doi.org/10.1007/s004180100303

Vancouver

Schüller U, Lamp EC, Schilling MK. Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex. HISTOCHEM CELL BIOL. 2001 Aug;116(2):149-59. https://doi.org/10.1007/s004180100303

Bibtex

@article{b0785798e4064d18a3b527bd3491da85,
title = "Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex",
abstract = "We utilized the murine cerebellum to analyze the expression of G-proteins during vertebrate neural differentiation. Combining reverse transcription-polymerase chain reaction and immunocytochemistry, we monitored the expression and cellular localization, within the nascent cerebellar cortex, of G-proteins subunits known to mediate signal transduction in the adult cerebellum. The mRNAs encoding subunits Galphaq, Galphao, Galphai-2, and Galphaz are expressed in the cerebellar anlage at least from embryonic day 14 onward, and relative levels of these mRNAs do not change appreciably from E14 to adulthood. Galphao, Galphaz, and Galphai-2 could be localized to granule cell neuroblasts and postmigratory, mature granule cells, but not to early postmitotic, premigratory, and migrating granule neurons. All of the Galpha subunits analyzed could also be localized to the cell somata of postmitotic Purkinje neurons, irrespective of age. In contrast, Purkinje cell dendrites stained for Galphao only up to postnatal day 8, dendritic immunoreactivity for Galphaz increased during dendritogenesis, and appreciable levels of Galphai-2 and Galphaq were seen in Purkinje cell dendrites only transiently during the 2nd and 3rd postnatal week. Of the G-beta and -gamma subunits analyzed (beta1, beta2, gamma2, gamma3, gamma5, and gamma7), only expression of gamma3 varied with development. It could be localized to Purkinje cell somata and dendrites in early postnatal, but not in adult animals. These changes in the cellular distribution and subcellular segregation of G-proteins are correlated to tangible aspects of cerebellar cortical histogenesis and suggest a role for G-protein-mediated signaling in their mechanistic implementation.",
keywords = "Age Factors, Amino Acid Sequence, Animals, Animals, Newborn, Cerebellar Cortex, DNA Primers, Female, Gene Expression Regulation, Developmental, Heterotrimeric GTP-Binding Proteins, Male, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Molecular Sequence Data, Phenotype, Pregnancy, Purkinje Cells, RNA, Messenger, Journal Article, Research Support, Non-U.S. Gov't",
author = "U Sch{\"u}ller and Lamp, {E C} and Schilling, {Martin K}",
year = "2001",
month = aug,
doi = "10.1007/s004180100303",
language = "English",
volume = "116",
pages = "149--59",
journal = "HISTOCHEM CELL BIOL",
issn = "0948-6143",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Developmental expression of heterotrimeric G-proteins in the murine cerebellar cortex

AU - Schüller, U

AU - Lamp, E C

AU - Schilling, Martin K

PY - 2001/8

Y1 - 2001/8

N2 - We utilized the murine cerebellum to analyze the expression of G-proteins during vertebrate neural differentiation. Combining reverse transcription-polymerase chain reaction and immunocytochemistry, we monitored the expression and cellular localization, within the nascent cerebellar cortex, of G-proteins subunits known to mediate signal transduction in the adult cerebellum. The mRNAs encoding subunits Galphaq, Galphao, Galphai-2, and Galphaz are expressed in the cerebellar anlage at least from embryonic day 14 onward, and relative levels of these mRNAs do not change appreciably from E14 to adulthood. Galphao, Galphaz, and Galphai-2 could be localized to granule cell neuroblasts and postmigratory, mature granule cells, but not to early postmitotic, premigratory, and migrating granule neurons. All of the Galpha subunits analyzed could also be localized to the cell somata of postmitotic Purkinje neurons, irrespective of age. In contrast, Purkinje cell dendrites stained for Galphao only up to postnatal day 8, dendritic immunoreactivity for Galphaz increased during dendritogenesis, and appreciable levels of Galphai-2 and Galphaq were seen in Purkinje cell dendrites only transiently during the 2nd and 3rd postnatal week. Of the G-beta and -gamma subunits analyzed (beta1, beta2, gamma2, gamma3, gamma5, and gamma7), only expression of gamma3 varied with development. It could be localized to Purkinje cell somata and dendrites in early postnatal, but not in adult animals. These changes in the cellular distribution and subcellular segregation of G-proteins are correlated to tangible aspects of cerebellar cortical histogenesis and suggest a role for G-protein-mediated signaling in their mechanistic implementation.

AB - We utilized the murine cerebellum to analyze the expression of G-proteins during vertebrate neural differentiation. Combining reverse transcription-polymerase chain reaction and immunocytochemistry, we monitored the expression and cellular localization, within the nascent cerebellar cortex, of G-proteins subunits known to mediate signal transduction in the adult cerebellum. The mRNAs encoding subunits Galphaq, Galphao, Galphai-2, and Galphaz are expressed in the cerebellar anlage at least from embryonic day 14 onward, and relative levels of these mRNAs do not change appreciably from E14 to adulthood. Galphao, Galphaz, and Galphai-2 could be localized to granule cell neuroblasts and postmigratory, mature granule cells, but not to early postmitotic, premigratory, and migrating granule neurons. All of the Galpha subunits analyzed could also be localized to the cell somata of postmitotic Purkinje neurons, irrespective of age. In contrast, Purkinje cell dendrites stained for Galphao only up to postnatal day 8, dendritic immunoreactivity for Galphaz increased during dendritogenesis, and appreciable levels of Galphai-2 and Galphaq were seen in Purkinje cell dendrites only transiently during the 2nd and 3rd postnatal week. Of the G-beta and -gamma subunits analyzed (beta1, beta2, gamma2, gamma3, gamma5, and gamma7), only expression of gamma3 varied with development. It could be localized to Purkinje cell somata and dendrites in early postnatal, but not in adult animals. These changes in the cellular distribution and subcellular segregation of G-proteins are correlated to tangible aspects of cerebellar cortical histogenesis and suggest a role for G-protein-mediated signaling in their mechanistic implementation.

KW - Age Factors

KW - Amino Acid Sequence

KW - Animals

KW - Animals, Newborn

KW - Cerebellar Cortex

KW - DNA Primers

KW - Female

KW - Gene Expression Regulation, Developmental

KW - Heterotrimeric GTP-Binding Proteins

KW - Male

KW - Mice

KW - Mice, Inbred C3H

KW - Mice, Inbred C57BL

KW - Molecular Sequence Data

KW - Phenotype

KW - Pregnancy

KW - Purkinje Cells

KW - RNA, Messenger

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/s004180100303

DO - 10.1007/s004180100303

M3 - SCORING: Journal article

C2 - 11685543

VL - 116

SP - 149

EP - 159

JO - HISTOCHEM CELL BIOL

JF - HISTOCHEM CELL BIOL

SN - 0948-6143

IS - 2

ER -