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Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients

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Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients. / Casagrande, Maria; Fitzek, Antonia; Püschel, Klaus; Aleshcheva, Ganna; Schultheiss, Heinz-Peter; Berneking, Laura; Spitzer, Martin S; Schultheiss, Maximilian.

In: OCUL IMMUNOL INFLAMM, Vol. 28, No. 5, 03.07.2020, p. 721-725.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Casagrande, M, Fitzek, A, Püschel, K, Aleshcheva, G, Schultheiss, H-P, Berneking, L, Spitzer, MS & Schultheiss, M 2020, 'Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients', OCUL IMMUNOL INFLAMM, vol. 28, no. 5, pp. 721-725. https://doi.org/10.1080/09273948.2020.1770301

APA

Casagrande, M., Fitzek, A., Püschel, K., Aleshcheva, G., Schultheiss, H-P., Berneking, L., Spitzer, M. S., & Schultheiss, M. (2020). Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients. OCUL IMMUNOL INFLAMM, 28(5), 721-725. https://doi.org/10.1080/09273948.2020.1770301

Vancouver

Casagrande M, Fitzek A, Püschel K, Aleshcheva G, Schultheiss H-P, Berneking L et al. Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients. OCUL IMMUNOL INFLAMM. 2020 Jul 3;28(5):721-725. https://doi.org/10.1080/09273948.2020.1770301

Bibtex

@article{e540537c5f3f4d27955d14d50c8fcc12,
title = "Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients",
abstract = "PURPOSE: To report the presence of viral ribonucleic acid (RNA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human retina in deceased patients with confirmed novel coronavirus disease 2019 (COVID-19).PATIENTS AND METHODS: Fourteen eyes of 14 deceased patients with confirmed COVID-19 disease were enucleated during autopsy. A sample of human retina was secured and fixed in RNAlater{\texttrademark}. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect three different viral RNA sequences (RdRp-gene, E-gene and Orf1 gene) of SARS-CoV-2.RESULTS: In three out of 14 eyes SARS-CoV-2 viral RNA was detected in the retina of deceased COVID-19 patients. As analysis for three different sequences (RdRp-gene, E-gene and Orf1 gene) revealed positive results in RT-PCR, the existence of SARS-CoV-2 viral RNA in human retina is proven according to the standards of the World-Health-Organization.CONCLUSION: Viral RNA of SARS-CoV-2 is detectable in the retina of COVID-19 patients.",
author = "Maria Casagrande and Antonia Fitzek and Klaus P{\"u}schel and Ganna Aleshcheva and Heinz-Peter Schultheiss and Laura Berneking and Spitzer, {Martin S} and Maximilian Schultheiss",
year = "2020",
month = jul,
day = "3",
doi = "10.1080/09273948.2020.1770301",
language = "English",
volume = "28",
pages = "721--725",
journal = "OCUL IMMUNOL INFLAMM",
issn = "0927-3948",
publisher = "informa healthcare",
number = "5",

}

RIS

TY - JOUR

T1 - Detection of SARS-CoV-2 in Human Retinal Biopsies of Deceased COVID-19 Patients

AU - Casagrande, Maria

AU - Fitzek, Antonia

AU - Püschel, Klaus

AU - Aleshcheva, Ganna

AU - Schultheiss, Heinz-Peter

AU - Berneking, Laura

AU - Spitzer, Martin S

AU - Schultheiss, Maximilian

PY - 2020/7/3

Y1 - 2020/7/3

N2 - PURPOSE: To report the presence of viral ribonucleic acid (RNA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human retina in deceased patients with confirmed novel coronavirus disease 2019 (COVID-19).PATIENTS AND METHODS: Fourteen eyes of 14 deceased patients with confirmed COVID-19 disease were enucleated during autopsy. A sample of human retina was secured and fixed in RNAlater™. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect three different viral RNA sequences (RdRp-gene, E-gene and Orf1 gene) of SARS-CoV-2.RESULTS: In three out of 14 eyes SARS-CoV-2 viral RNA was detected in the retina of deceased COVID-19 patients. As analysis for three different sequences (RdRp-gene, E-gene and Orf1 gene) revealed positive results in RT-PCR, the existence of SARS-CoV-2 viral RNA in human retina is proven according to the standards of the World-Health-Organization.CONCLUSION: Viral RNA of SARS-CoV-2 is detectable in the retina of COVID-19 patients.

AB - PURPOSE: To report the presence of viral ribonucleic acid (RNA) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human retina in deceased patients with confirmed novel coronavirus disease 2019 (COVID-19).PATIENTS AND METHODS: Fourteen eyes of 14 deceased patients with confirmed COVID-19 disease were enucleated during autopsy. A sample of human retina was secured and fixed in RNAlater™. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to detect three different viral RNA sequences (RdRp-gene, E-gene and Orf1 gene) of SARS-CoV-2.RESULTS: In three out of 14 eyes SARS-CoV-2 viral RNA was detected in the retina of deceased COVID-19 patients. As analysis for three different sequences (RdRp-gene, E-gene and Orf1 gene) revealed positive results in RT-PCR, the existence of SARS-CoV-2 viral RNA in human retina is proven according to the standards of the World-Health-Organization.CONCLUSION: Viral RNA of SARS-CoV-2 is detectable in the retina of COVID-19 patients.

U2 - 10.1080/09273948.2020.1770301

DO - 10.1080/09273948.2020.1770301

M3 - SCORING: Journal article

C2 - 32469258

VL - 28

SP - 721

EP - 725

JO - OCUL IMMUNOL INFLAMM

JF - OCUL IMMUNOL INFLAMM

SN - 0927-3948

IS - 5

ER -