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Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19

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Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19. / Casagrande, Maria; Fitzek, Antonia; Spitzer, Martin; Püschel, Klaus; Glatzel, Markus; Krasemann, Susanne; Aepfelbacher, Martin; Nörz, Dominik; Lütgehetmann, Marc; Pfefferle, Susanne; Spitzer, Martin.

In: BRIT J OPHTHALMOL, Vol. 106, No. 9, 09.2022, p. 1313-1317.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Casagrande, M, Fitzek, A, Spitzer, M, Püschel, K, Glatzel, M, Krasemann, S, Aepfelbacher, M, Nörz, D, Lütgehetmann, M, Pfefferle, S & Spitzer, M 2022, 'Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19', BRIT J OPHTHALMOL, vol. 106, no. 9, pp. 1313-1317. https://doi.org/10.1136/bjophthalmol-2020-318618

APA

Casagrande, M., Fitzek, A., Spitzer, M., Püschel, K., Glatzel, M., Krasemann, S., Aepfelbacher, M., Nörz, D., Lütgehetmann, M., Pfefferle, S., & Spitzer, M. (2022). Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19. BRIT J OPHTHALMOL, 106(9), 1313-1317. https://doi.org/10.1136/bjophthalmol-2020-318618

Vancouver

Casagrande M, Fitzek A, Spitzer M, Püschel K, Glatzel M, Krasemann S et al. Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19. BRIT J OPHTHALMOL. 2022 Sep;106(9):1313-1317. https://doi.org/10.1136/bjophthalmol-2020-318618

Bibtex

@article{25aaf3e60ef049e09ceada1e50a7351b,
title = "Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19",
abstract = "PURPOSE: Presence of SARS-CoV-2 RNA in human retinal biopsies (RBs) was previously reported by us. In this consecutive study, we analysed RB and optic nerve biopsies (ONBs) in deceased patients with confirmed COVID-19 assessing viral RNA load, possible virus replication and infectivity.PATIENTS AND METHODS: In this case series, 14 eyes of 14 deceased patients with COVID-19 were enucleated during autopsy. RB and ONB were subjected to molecular detection of viral RNA, virus cultivation and immunohistochemistry. SARS-CoV-2 RNA loads were compared with RNA loads in the respective throat swabs, vitreous humour and blood samples.RESULTS: SARS-CoV-2 RNA was detected in 7/14 RBs and in 10/13 ONBs. While virus isolation failed and immunohistochemistry of SARS-CoV-2 spike protein was negative, subgenomic RNA (sgRNA) was detectable (40% RB; 60% ONB).CONCLUSION: SARS-CoV-2 RNA is detectable in RB and ONB of patients with COVID-19. Presence of sgRNA could point to a SARS-CoV-2 infection of neuronal tissue, but as virus isolation failed and immunohistochemistry of SARS-CoV-2 spike protein was negative, an active infection seems unlikely.",
author = "Maria Casagrande and Antonia Fitzek and Martin Spitzer and Klaus P{\"u}schel and Markus Glatzel and Susanne Krasemann and Martin Aepfelbacher and Dominik N{\"o}rz and Marc L{\"u}tgehetmann and Susanne Pfefferle and Martin Spitzer",
note = "{\textcopyright} Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.",
year = "2022",
month = sep,
doi = "10.1136/bjophthalmol-2020-318618",
language = "English",
volume = "106",
pages = "1313--1317",
journal = "BRIT J OPHTHALMOL",
issn = "0007-1161",
publisher = "BMJ PUBLISHING GROUP",
number = "9",

}

RIS

TY - JOUR

T1 - Detection of SARS-CoV-2 genomic and subgenomic RNA in retina and optic nerve of patients with COVID-19

AU - Casagrande, Maria

AU - Fitzek, Antonia

AU - Spitzer, Martin

AU - Püschel, Klaus

AU - Glatzel, Markus

AU - Krasemann, Susanne

AU - Aepfelbacher, Martin

AU - Nörz, Dominik

AU - Lütgehetmann, Marc

AU - Pfefferle, Susanne

AU - Spitzer, Martin

N1 - © Author(s) (or their employer(s)) 2021. No commercial re-use. See rights and permissions. Published by BMJ.

PY - 2022/9

Y1 - 2022/9

N2 - PURPOSE: Presence of SARS-CoV-2 RNA in human retinal biopsies (RBs) was previously reported by us. In this consecutive study, we analysed RB and optic nerve biopsies (ONBs) in deceased patients with confirmed COVID-19 assessing viral RNA load, possible virus replication and infectivity.PATIENTS AND METHODS: In this case series, 14 eyes of 14 deceased patients with COVID-19 were enucleated during autopsy. RB and ONB were subjected to molecular detection of viral RNA, virus cultivation and immunohistochemistry. SARS-CoV-2 RNA loads were compared with RNA loads in the respective throat swabs, vitreous humour and blood samples.RESULTS: SARS-CoV-2 RNA was detected in 7/14 RBs and in 10/13 ONBs. While virus isolation failed and immunohistochemistry of SARS-CoV-2 spike protein was negative, subgenomic RNA (sgRNA) was detectable (40% RB; 60% ONB).CONCLUSION: SARS-CoV-2 RNA is detectable in RB and ONB of patients with COVID-19. Presence of sgRNA could point to a SARS-CoV-2 infection of neuronal tissue, but as virus isolation failed and immunohistochemistry of SARS-CoV-2 spike protein was negative, an active infection seems unlikely.

AB - PURPOSE: Presence of SARS-CoV-2 RNA in human retinal biopsies (RBs) was previously reported by us. In this consecutive study, we analysed RB and optic nerve biopsies (ONBs) in deceased patients with confirmed COVID-19 assessing viral RNA load, possible virus replication and infectivity.PATIENTS AND METHODS: In this case series, 14 eyes of 14 deceased patients with COVID-19 were enucleated during autopsy. RB and ONB were subjected to molecular detection of viral RNA, virus cultivation and immunohistochemistry. SARS-CoV-2 RNA loads were compared with RNA loads in the respective throat swabs, vitreous humour and blood samples.RESULTS: SARS-CoV-2 RNA was detected in 7/14 RBs and in 10/13 ONBs. While virus isolation failed and immunohistochemistry of SARS-CoV-2 spike protein was negative, subgenomic RNA (sgRNA) was detectable (40% RB; 60% ONB).CONCLUSION: SARS-CoV-2 RNA is detectable in RB and ONB of patients with COVID-19. Presence of sgRNA could point to a SARS-CoV-2 infection of neuronal tissue, but as virus isolation failed and immunohistochemistry of SARS-CoV-2 spike protein was negative, an active infection seems unlikely.

U2 - 10.1136/bjophthalmol-2020-318618

DO - 10.1136/bjophthalmol-2020-318618

M3 - SCORING: Journal article

C2 - 33836988

VL - 106

SP - 1313

EP - 1317

JO - BRIT J OPHTHALMOL

JF - BRIT J OPHTHALMOL

SN - 0007-1161

IS - 9

ER -