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Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome

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Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome. / Scholz, Philipp; Auler, Markus; Ruthard, Johannes; Brachvogel, Bent; Klatt, Andreas R; Streichert, Thomas.

In: BIOCHEM MEDICA, Vol. 28, No. 3, 15.10.2018, p. 030703.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Scholz, P, Auler, M, Ruthard, J, Brachvogel, B, Klatt, AR & Streichert, T 2018, 'Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome', BIOCHEM MEDICA, vol. 28, no. 3, pp. 030703. https://doi.org/10.11613/BM.2018.030703

APA

Scholz, P., Auler, M., Ruthard, J., Brachvogel, B., Klatt, A. R., & Streichert, T. (2018). Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome. BIOCHEM MEDICA, 28(3), 030703. https://doi.org/10.11613/BM.2018.030703

Vancouver

Scholz P, Auler M, Ruthard J, Brachvogel B, Klatt AR, Streichert T. Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome. BIOCHEM MEDICA. 2018 Oct 15;28(3):030703. https://doi.org/10.11613/BM.2018.030703

Bibtex

@article{91b42b59be1f47e0a691296a65bdd1d0,
title = "Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome",
abstract = "Introduction: Antibodies specific for annexin A8 (AnxA8) have not been investigated in patients suffering from antiphospholipid syndrome (APS) yet. The aim of this study was to compare the presence of AnxA8 antibodies in serum of APS patients with that of age-matched healthy controls and to investigate whether AnxA8 antibodies are potential biomarkers for APS.Materials and methods: We enrolled 22 APS patients and 22 healthy controls in this case-control study. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblot to investigate the presence of AnxA8 antibodies, and we applied enzyme-linked immunosorbent assay to investigate the presence of cardiolipin (CL) and beta-2-glycoprotein I ({\ss}2GPI) antibodies.Results: The serum of 9/22 APS patients showed AnxA8 IgG isotype antibody reactivity compared to serum of 2/22 healthy controls (P = 0.034). When we also included weak immunoblot signals, 12/22 APS patients exhibited AnxA8 IgG isotype antibody reactivity compared to 3/22 healthy controls (P = 0.005). We also investigated the presence of AnxA8 IgM isotype antibodies in the serum of APS patients but found no statistically significant difference between the APS patient group and healthy control group (P = 0.500). We further investigated the presence of {\ss}2GPI and CL IgG and IgM isotype antibodies. AnxA8 IgG isotype antibodies were present in APS patients in a similar frequency as the APS {"}criteria{"} antibody against CL (P = 0.764).Conclusion: We demonstrated that AnxA8 IgG isotype antibodies are potential biomarkers for the diagnosis of APS.",
keywords = "Journal Article",
author = "Philipp Scholz and Markus Auler and Johannes Ruthard and Bent Brachvogel and Klatt, {Andreas R} and Thomas Streichert",
year = "2018",
month = oct,
day = "15",
doi = "10.11613/BM.2018.030703",
language = "English",
volume = "28",
pages = "030703",
journal = "BIOCHEM MEDICA",
issn = "1330-0962",
publisher = "Biochemia Medica, Editorial Office",
number = "3",

}

RIS

TY - JOUR

T1 - Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome

AU - Scholz, Philipp

AU - Auler, Markus

AU - Ruthard, Johannes

AU - Brachvogel, Bent

AU - Klatt, Andreas R

AU - Streichert, Thomas

PY - 2018/10/15

Y1 - 2018/10/15

N2 - Introduction: Antibodies specific for annexin A8 (AnxA8) have not been investigated in patients suffering from antiphospholipid syndrome (APS) yet. The aim of this study was to compare the presence of AnxA8 antibodies in serum of APS patients with that of age-matched healthy controls and to investigate whether AnxA8 antibodies are potential biomarkers for APS.Materials and methods: We enrolled 22 APS patients and 22 healthy controls in this case-control study. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblot to investigate the presence of AnxA8 antibodies, and we applied enzyme-linked immunosorbent assay to investigate the presence of cardiolipin (CL) and beta-2-glycoprotein I (ß2GPI) antibodies.Results: The serum of 9/22 APS patients showed AnxA8 IgG isotype antibody reactivity compared to serum of 2/22 healthy controls (P = 0.034). When we also included weak immunoblot signals, 12/22 APS patients exhibited AnxA8 IgG isotype antibody reactivity compared to 3/22 healthy controls (P = 0.005). We also investigated the presence of AnxA8 IgM isotype antibodies in the serum of APS patients but found no statistically significant difference between the APS patient group and healthy control group (P = 0.500). We further investigated the presence of ß2GPI and CL IgG and IgM isotype antibodies. AnxA8 IgG isotype antibodies were present in APS patients in a similar frequency as the APS "criteria" antibody against CL (P = 0.764).Conclusion: We demonstrated that AnxA8 IgG isotype antibodies are potential biomarkers for the diagnosis of APS.

AB - Introduction: Antibodies specific for annexin A8 (AnxA8) have not been investigated in patients suffering from antiphospholipid syndrome (APS) yet. The aim of this study was to compare the presence of AnxA8 antibodies in serum of APS patients with that of age-matched healthy controls and to investigate whether AnxA8 antibodies are potential biomarkers for APS.Materials and methods: We enrolled 22 APS patients and 22 healthy controls in this case-control study. We used sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblot to investigate the presence of AnxA8 antibodies, and we applied enzyme-linked immunosorbent assay to investigate the presence of cardiolipin (CL) and beta-2-glycoprotein I (ß2GPI) antibodies.Results: The serum of 9/22 APS patients showed AnxA8 IgG isotype antibody reactivity compared to serum of 2/22 healthy controls (P = 0.034). When we also included weak immunoblot signals, 12/22 APS patients exhibited AnxA8 IgG isotype antibody reactivity compared to 3/22 healthy controls (P = 0.005). We also investigated the presence of AnxA8 IgM isotype antibodies in the serum of APS patients but found no statistically significant difference between the APS patient group and healthy control group (P = 0.500). We further investigated the presence of ß2GPI and CL IgG and IgM isotype antibodies. AnxA8 IgG isotype antibodies were present in APS patients in a similar frequency as the APS "criteria" antibody against CL (P = 0.764).Conclusion: We demonstrated that AnxA8 IgG isotype antibodies are potential biomarkers for the diagnosis of APS.

KW - Journal Article

U2 - 10.11613/BM.2018.030703

DO - 10.11613/BM.2018.030703

M3 - SCORING: Journal article

C2 - 30429671

VL - 28

SP - 030703

JO - BIOCHEM MEDICA

JF - BIOCHEM MEDICA

SN - 1330-0962

IS - 3

ER -