DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth
Related Research units
Abstract
During cap-dependent eukaryotic translation initiation, ribosomes scan messenger RNA from the 5' end to the first AUG start codon with favourable sequence context. For many mRNAs this AUG belongs to a short upstream open reading frame (uORF), and translation of the main downstream ORF requires re-initiation, an incompletely understood process. Re-initiation is thought to involve the same factors as standard initiation. It is unknown whether any factors specifically affect translation re-initiation without affecting standard cap-dependent translation. Here we uncover the non-canonical initiation factors density regulated protein (DENR) and multiple copies in T-cell lymphoma-1 (MCT-1; also called MCTS1 in humans) as the first selective regulators of eukaryotic re-initiation. mRNAs containing upstream ORFs with strong Kozak sequences selectively require DENR-MCT-1 for their proper translation, yielding a novel class of mRNAs that can be co-regulated and that is enriched for regulatory proteins such as oncogenic kinases. Collectively, our data reveal that cells have a previously unappreciated translational control system with a key role in supporting proliferation and tissue growth.
Bibliographical data
Original language | English |
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ISSN | 0028-0836 |
DOIs | |
Publication status | Published - 14.08.2014 |
Comment Deanary
UKE Publication of the Month August 2014
Sybille Schleich, Philipp Christoph Janiesch, Katharine K Miller and Katrin Küchler should all be affiliated with "Sonstige MitarbeiterInnen und Einrichtungen des ZMNH"
PubMed | 25043021 |
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