Decrease in circulating dendritic cell precursors in patients with peripheral artery disease
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Decrease in circulating dendritic cell precursors in patients with peripheral artery disease. / Kretzschmar, Daniel; Rohm, Ilonka; Schäller, Sebastian; Betge, Stefan; Pistulli, Rudin; Atiskova, Yevgeniya; Figulla, Hans-R; Yilmaz, Atilla.
In: MEDIAT INFLAMM, Vol. 2015, 2015, p. 450957.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Decrease in circulating dendritic cell precursors in patients with peripheral artery disease
AU - Kretzschmar, Daniel
AU - Rohm, Ilonka
AU - Schäller, Sebastian
AU - Betge, Stefan
AU - Pistulli, Rudin
AU - Atiskova, Yevgeniya
AU - Figulla, Hans-R
AU - Yilmaz, Atilla
PY - 2015
Y1 - 2015
N2 - Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Therefore, we hypothesize that, in patients with PAD, DCPs might be reduced in blood due to their recruitment into the vascular wall and induce a proinflammatory response. The numbers of myeloid DCPs, plasmacytoid DCPs, and total DCPs were analyzed by flow cytometry in blood of patients with PAD (n = 52) compared to controls (n = 60). Femoralis plaques (n = 12) of patients who underwent surgery were immunostained for CD209 and CD83 (mDCs) as well as CD304, CD123 (pDCs), and HLA-DR. In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed. In immunostaining, markers indicative for mDCs (CD209: 16 versus 8 cells/0.1 mm(2), P = 0.02; CD83: 19 versus 5 cells/0.1 mm(2), P = 0.03) were significantly elevated in femoralis plaques compared to control vessels. We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD. Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques.
AB - Peripheral artery disease (PAD) is a common manifestation of atherosclerosis. Inflammation is important for initiation and progression of the disease. Dendritic cells (DCs) as antigen-presenting cells play an important role in the immune system. Therefore, we hypothesize that, in patients with PAD, DCPs might be reduced in blood due to their recruitment into the vascular wall and induce a proinflammatory response. The numbers of myeloid DCPs, plasmacytoid DCPs, and total DCPs were analyzed by flow cytometry in blood of patients with PAD (n = 52) compared to controls (n = 60). Femoralis plaques (n = 12) of patients who underwent surgery were immunostained for CD209 and CD83 (mDCs) as well as CD304, CD123 (pDCs), and HLA-DR. In patients with PAD, a significant decrease in mDCPs, pDCPs, and tDCPs was observed. In immunostaining, markers indicative for mDCs (CD209: 16 versus 8 cells/0.1 mm(2), P = 0.02; CD83: 19 versus 5 cells/0.1 mm(2), P = 0.03) were significantly elevated in femoralis plaques compared to control vessels. We show for the first time that mDCPs, pDCPs, and tDCPs are significantly reduced in patients with PAD. Immunohistochemical analysis unraveled that the decrease in DCPs might be due to their recruitment into atherosclerotic plaques.
KW - Adult
KW - Aged
KW - Atherosclerosis/blood
KW - Case-Control Studies
KW - Cell Separation
KW - Dendritic Cells/cytology
KW - Echocardiography
KW - Female
KW - Flow Cytometry
KW - Humans
KW - Immunohistochemistry
KW - Inflammation/immunology
KW - Male
KW - Middle Aged
KW - Myeloid Cells/cytology
KW - Peripheral Arterial Disease/blood
KW - Plaque, Atherosclerotic/immunology
U2 - 10.1155/2015/450957
DO - 10.1155/2015/450957
M3 - SCORING: Journal article
C2 - 25960616
VL - 2015
SP - 450957
JO - MEDIAT INFLAMM
JF - MEDIAT INFLAMM
SN - 0962-9351
ER -