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Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment

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Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment. / Huttala, Outi; Loreth, Desiree; Staff, Synnöve; Tanner, Minna; Wikman, Harriet; Ylikomi, Timo.

In: BIOMEDICINES, Vol. 10, No. 2, 271, 26.01.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Huttala, O, Loreth, D, Staff, S, Tanner, M, Wikman, H & Ylikomi, T 2022, 'Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment', BIOMEDICINES, vol. 10, no. 2, 271. https://doi.org/10.3390/biomedicines10020271

APA

Huttala, O., Loreth, D., Staff, S., Tanner, M., Wikman, H., & Ylikomi, T. (2022). Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment. BIOMEDICINES, 10(2), [271]. https://doi.org/10.3390/biomedicines10020271

Vancouver

Huttala O, Loreth D, Staff S, Tanner M, Wikman H, Ylikomi T. Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment. BIOMEDICINES. 2022 Jan 26;10(2). 271. https://doi.org/10.3390/biomedicines10020271

Bibtex

@article{4c439113cd0249d38c4d40909b7b8753,
title = "Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment",
abstract = "Vascularization plays an important role in the microenvironment of the tumor. Therefore, it should be a key element to be considered in the development of in vitro cancer assays. In this study, we decellularized in vitro capillaries to remove genetic material and optimized the medium used to increase the robustness and versatility of applications. The growth pattern and drug responses of cancer cell lines and patient-derived primary cells were studied on decellularized capillaries. Interestingly, two distinct growth patterns were seen when cancer cells were grown on decellularized capillaries: {"}network{"} and {"}cluster{"}. Network formation correlated with the metastatic properties of the cells and cluster formation was observed in non-metastatic cells. Drug responses of patient-derived cells correlated better with clinical findings when cells were cultured on decellularized capillaries compared with those cultured on plastic. Decellularized capillaries provide a novel method for cancer cell culture applications. It bridges the gap between complex 3D culture methods and traditional 2D culture methods by providing the ease and robustness of 2D culture as well as an in vivo-like microenvironment and scaffolding for 3D cultures.",
author = "Outi Huttala and Desiree Loreth and Synn{\"o}ve Staff and Minna Tanner and Harriet Wikman and Timo Ylikomi",
year = "2022",
month = jan,
day = "26",
doi = "10.3390/biomedicines10020271",
language = "English",
volume = "10",
journal = "BIOMEDICINES",
issn = "2227-9059",
publisher = "MDPI AG",
number = "2",

}

RIS

TY - JOUR

T1 - Decellularized In Vitro Capillaries for Studies of Metastatic Tendency and Selection of Treatment

AU - Huttala, Outi

AU - Loreth, Desiree

AU - Staff, Synnöve

AU - Tanner, Minna

AU - Wikman, Harriet

AU - Ylikomi, Timo

PY - 2022/1/26

Y1 - 2022/1/26

N2 - Vascularization plays an important role in the microenvironment of the tumor. Therefore, it should be a key element to be considered in the development of in vitro cancer assays. In this study, we decellularized in vitro capillaries to remove genetic material and optimized the medium used to increase the robustness and versatility of applications. The growth pattern and drug responses of cancer cell lines and patient-derived primary cells were studied on decellularized capillaries. Interestingly, two distinct growth patterns were seen when cancer cells were grown on decellularized capillaries: "network" and "cluster". Network formation correlated with the metastatic properties of the cells and cluster formation was observed in non-metastatic cells. Drug responses of patient-derived cells correlated better with clinical findings when cells were cultured on decellularized capillaries compared with those cultured on plastic. Decellularized capillaries provide a novel method for cancer cell culture applications. It bridges the gap between complex 3D culture methods and traditional 2D culture methods by providing the ease and robustness of 2D culture as well as an in vivo-like microenvironment and scaffolding for 3D cultures.

AB - Vascularization plays an important role in the microenvironment of the tumor. Therefore, it should be a key element to be considered in the development of in vitro cancer assays. In this study, we decellularized in vitro capillaries to remove genetic material and optimized the medium used to increase the robustness and versatility of applications. The growth pattern and drug responses of cancer cell lines and patient-derived primary cells were studied on decellularized capillaries. Interestingly, two distinct growth patterns were seen when cancer cells were grown on decellularized capillaries: "network" and "cluster". Network formation correlated with the metastatic properties of the cells and cluster formation was observed in non-metastatic cells. Drug responses of patient-derived cells correlated better with clinical findings when cells were cultured on decellularized capillaries compared with those cultured on plastic. Decellularized capillaries provide a novel method for cancer cell culture applications. It bridges the gap between complex 3D culture methods and traditional 2D culture methods by providing the ease and robustness of 2D culture as well as an in vivo-like microenvironment and scaffolding for 3D cultures.

U2 - 10.3390/biomedicines10020271

DO - 10.3390/biomedicines10020271

M3 - SCORING: Journal article

C2 - 35203480

VL - 10

JO - BIOMEDICINES

JF - BIOMEDICINES

SN - 2227-9059

IS - 2

M1 - 271

ER -