DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues.
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DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues. / Bogoevska, Valentina; Nollau, Peter; Lucka, Lothar; Grunow, Detlef; Klampe, Birgit; Uotila, Liisa M; Samsen, Alexandra; Gahmberg, Carl G; Wagener, Christoph.
In: GLYCOBIOLOGY, Vol. 17, No. 3, 3, 2007, p. 324-333.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - DC-SIGN binds ICAM-3 isolated from peripheral human leukocytes through Lewis x residues.
AU - Bogoevska, Valentina
AU - Nollau, Peter
AU - Lucka, Lothar
AU - Grunow, Detlef
AU - Klampe, Birgit
AU - Uotila, Liisa M
AU - Samsen, Alexandra
AU - Gahmberg, Carl G
AU - Wagener, Christoph
PY - 2007
Y1 - 2007
N2 - Intercellular adhesion molecule-3 (ICAM-3) binds to the alpha(L)beta(2) integrin and mediates the contact between T cells and antigen-presenting cells. It has been suggested that dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a C-type lectin of macrophages and DCs, is an additional ligand of ICAM-3. So far, the glycan structure mediating the interaction of native ICAM-3 with DC-SIGN is undefined. Here, we demonstrate that native ICAM-3 from human peripheral leukocytes binds recombinant DC-SIGN, is recognized by monoclonal Lewis x antibodies, and specifically interacts with DC-SIGN on immature DCs. The presence of Lewis x residues on ICAM-3 was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy. Investigations on different peripheral blood cell populations revealed that only ICAM-3 from granulocytes bound DC-SIGN. Cotransfection studies demonstrated that fucosyltransferase (FUT) IX and, to a significantly lesser extent, FUT IV, but not FUTs III and VII, mediate the synthesis of Lewis x residues on ICAM-3. These findings indicate that FUT IX is the main FUT mediating the synthesis of Lewis x residues of ICAM-3 in cells of the myeloid lineage, and that these residues bind DC-SIGN. The results suggest that ICAM-3 assists in the interaction of granulocytes with DC-SIGN of DCs.
AB - Intercellular adhesion molecule-3 (ICAM-3) binds to the alpha(L)beta(2) integrin and mediates the contact between T cells and antigen-presenting cells. It has been suggested that dendritic cell-specific ICAM-3 grabbing nonintegrin (DC-SIGN), a C-type lectin of macrophages and DCs, is an additional ligand of ICAM-3. So far, the glycan structure mediating the interaction of native ICAM-3 with DC-SIGN is undefined. Here, we demonstrate that native ICAM-3 from human peripheral leukocytes binds recombinant DC-SIGN, is recognized by monoclonal Lewis x antibodies, and specifically interacts with DC-SIGN on immature DCs. The presence of Lewis x residues on ICAM-3 was confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy. Investigations on different peripheral blood cell populations revealed that only ICAM-3 from granulocytes bound DC-SIGN. Cotransfection studies demonstrated that fucosyltransferase (FUT) IX and, to a significantly lesser extent, FUT IV, but not FUTs III and VII, mediate the synthesis of Lewis x residues on ICAM-3. These findings indicate that FUT IX is the main FUT mediating the synthesis of Lewis x residues of ICAM-3 in cells of the myeloid lineage, and that these residues bind DC-SIGN. The results suggest that ICAM-3 assists in the interaction of granulocytes with DC-SIGN of DCs.
M3 - SCORING: Zeitschriftenaufsatz
VL - 17
SP - 324
EP - 333
JO - GLYCOBIOLOGY
JF - GLYCOBIOLOGY
SN - 0959-6658
IS - 3
M1 - 3
ER -
