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Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells

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Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells : artificial APCs pave the way for clinical application by potent primary in vitro induction. / Schilbach, Karin; Kerst, Gunter; Walter, Steffen; Eyrich, Matthias; Wernet, Dorothee; Handgretinger, Rupert; Xie, Weidong; Rammensee, Hans-Georg; Müller, Ingo; Bühring, Hans-Jörg; Niethammer, Dietrich.

In: BLOOD, Vol. 106, No. 1, 01.07.2005, p. 144-9.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schilbach, K, Kerst, G, Walter, S, Eyrich, M, Wernet, D, Handgretinger, R, Xie, W, Rammensee, H-G, Müller, I, Bühring, H-J & Niethammer, D 2005, 'Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells: artificial APCs pave the way for clinical application by potent primary in vitro induction', BLOOD, vol. 106, no. 1, pp. 144-9. https://doi.org/10.1182/blood-2004-07-2940

APA

Schilbach, K., Kerst, G., Walter, S., Eyrich, M., Wernet, D., Handgretinger, R., Xie, W., Rammensee, H-G., Müller, I., Bühring, H-J., & Niethammer, D. (2005). Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells: artificial APCs pave the way for clinical application by potent primary in vitro induction. BLOOD, 106(1), 144-9. https://doi.org/10.1182/blood-2004-07-2940

Vancouver

Schilbach K, Kerst G, Walter S, Eyrich M, Wernet D, Handgretinger R et al. Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells: artificial APCs pave the way for clinical application by potent primary in vitro induction. BLOOD. 2005 Jul 1;106(1):144-9. https://doi.org/10.1182/blood-2004-07-2940

Bibtex

@article{bdbee079b1624d829f26a9707f703d65,
title = "Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells: artificial APCs pave the way for clinical application by potent primary in vitro induction",
abstract = "Induction of cytotoxic T lymphocytes (CTLs) for treatment of relapsed leukemia after allogeneic stem-cell transplantation is hindered by the laborious and time-consuming procedure of generating dendritic cells for antigen presentation. Artificial antigen-presenting cells (aAPCs) offer the advantage of being readily available in sufficient numbers, thus allowing for a highly standardized in vitro induction of CTLs. We generated aAPCs coated with anti-CD28 antibody (Ab) and either high-density (HD) or low-density (LD) major histocompatibility complex (MHC) class I molecules loaded with HA-1(H), a nonapeptide derived from the hematopoiesis-restricted minor histocompatibility antigen HA-1. HD- and LD-aAPCs potently induced HA-1(H)-specific CD8+ CTLs from untouched CD8+ T cells of healthy donors. CTLs were subsequently purified by magnetic-activated cell sorting. HD- as well as LD-aAPC-induced CTLs exerted high HA-1H-specific cytotoxicity, resembled T(c)1 effector memory cells, survived a long time in vitro, and were expanded by a factor varying between 8.2 x 10(4) and 51 x 10(4). The T-cell receptor (TCR) repertoire of HA-1H tetramer-positive CTLs was oligoclonal with a prominent usage of Vbeta6. The TCR repertoire of tetramer-positive CTLs was distinct from and more restricted than that of tetramer-negative cells. These findings indicate that aAPCs are attractive tools for the ex vivo generation of HA-1H-specific CTLs suitable for immunotherapy of relapsed leukemia.",
keywords = "Adoptive Transfer, Antigen-Presenting Cells, B-Lymphocytes, Blood Donors, CD8-Positive T-Lymphocytes, Cell Line, Transformed, Child, Female, Humans, Immunologic Memory, In Vitro Techniques, Male, Minor Histocompatibility Antigens, Oligopeptides, Precursor Cell Lymphoblastic Leukemia-Lymphoma, T-Lymphocytes, Cytotoxic",
author = "Karin Schilbach and Gunter Kerst and Steffen Walter and Matthias Eyrich and Dorothee Wernet and Rupert Handgretinger and Weidong Xie and Hans-Georg Rammensee and Ingo M{\"u}ller and Hans-J{\"o}rg B{\"u}hring and Dietrich Niethammer",
year = "2005",
month = jul,
day = "1",
doi = "10.1182/blood-2004-07-2940",
language = "English",
volume = "106",
pages = "144--9",
journal = "BLOOD",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

RIS

TY - JOUR

T1 - Cytotoxic minor histocompatibility antigen HA-1-specific CD8+ effector memory T cells

T2 - artificial APCs pave the way for clinical application by potent primary in vitro induction

AU - Schilbach, Karin

AU - Kerst, Gunter

AU - Walter, Steffen

AU - Eyrich, Matthias

AU - Wernet, Dorothee

AU - Handgretinger, Rupert

AU - Xie, Weidong

AU - Rammensee, Hans-Georg

AU - Müller, Ingo

AU - Bühring, Hans-Jörg

AU - Niethammer, Dietrich

PY - 2005/7/1

Y1 - 2005/7/1

N2 - Induction of cytotoxic T lymphocytes (CTLs) for treatment of relapsed leukemia after allogeneic stem-cell transplantation is hindered by the laborious and time-consuming procedure of generating dendritic cells for antigen presentation. Artificial antigen-presenting cells (aAPCs) offer the advantage of being readily available in sufficient numbers, thus allowing for a highly standardized in vitro induction of CTLs. We generated aAPCs coated with anti-CD28 antibody (Ab) and either high-density (HD) or low-density (LD) major histocompatibility complex (MHC) class I molecules loaded with HA-1(H), a nonapeptide derived from the hematopoiesis-restricted minor histocompatibility antigen HA-1. HD- and LD-aAPCs potently induced HA-1(H)-specific CD8+ CTLs from untouched CD8+ T cells of healthy donors. CTLs were subsequently purified by magnetic-activated cell sorting. HD- as well as LD-aAPC-induced CTLs exerted high HA-1H-specific cytotoxicity, resembled T(c)1 effector memory cells, survived a long time in vitro, and were expanded by a factor varying between 8.2 x 10(4) and 51 x 10(4). The T-cell receptor (TCR) repertoire of HA-1H tetramer-positive CTLs was oligoclonal with a prominent usage of Vbeta6. The TCR repertoire of tetramer-positive CTLs was distinct from and more restricted than that of tetramer-negative cells. These findings indicate that aAPCs are attractive tools for the ex vivo generation of HA-1H-specific CTLs suitable for immunotherapy of relapsed leukemia.

AB - Induction of cytotoxic T lymphocytes (CTLs) for treatment of relapsed leukemia after allogeneic stem-cell transplantation is hindered by the laborious and time-consuming procedure of generating dendritic cells for antigen presentation. Artificial antigen-presenting cells (aAPCs) offer the advantage of being readily available in sufficient numbers, thus allowing for a highly standardized in vitro induction of CTLs. We generated aAPCs coated with anti-CD28 antibody (Ab) and either high-density (HD) or low-density (LD) major histocompatibility complex (MHC) class I molecules loaded with HA-1(H), a nonapeptide derived from the hematopoiesis-restricted minor histocompatibility antigen HA-1. HD- and LD-aAPCs potently induced HA-1(H)-specific CD8+ CTLs from untouched CD8+ T cells of healthy donors. CTLs were subsequently purified by magnetic-activated cell sorting. HD- as well as LD-aAPC-induced CTLs exerted high HA-1H-specific cytotoxicity, resembled T(c)1 effector memory cells, survived a long time in vitro, and were expanded by a factor varying between 8.2 x 10(4) and 51 x 10(4). The T-cell receptor (TCR) repertoire of HA-1H tetramer-positive CTLs was oligoclonal with a prominent usage of Vbeta6. The TCR repertoire of tetramer-positive CTLs was distinct from and more restricted than that of tetramer-negative cells. These findings indicate that aAPCs are attractive tools for the ex vivo generation of HA-1H-specific CTLs suitable for immunotherapy of relapsed leukemia.

KW - Adoptive Transfer

KW - Antigen-Presenting Cells

KW - B-Lymphocytes

KW - Blood Donors

KW - CD8-Positive T-Lymphocytes

KW - Cell Line, Transformed

KW - Child

KW - Female

KW - Humans

KW - Immunologic Memory

KW - In Vitro Techniques

KW - Male

KW - Minor Histocompatibility Antigens

KW - Oligopeptides

KW - Precursor Cell Lymphoblastic Leukemia-Lymphoma

KW - T-Lymphocytes, Cytotoxic

U2 - 10.1182/blood-2004-07-2940

DO - 10.1182/blood-2004-07-2940

M3 - SCORING: Journal article

C2 - 15731181

VL - 106

SP - 144

EP - 149

JO - BLOOD

JF - BLOOD

SN - 0006-4971

IS - 1

ER -