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CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats

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CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats. / Fritzsche, Britta; Schuchardt, Jan-Philipp; Schmidt, Anja; Nau, Heinz; Schweigert, Florian J; Rühl, Ralph.

In: REPROD TOXICOL, Vol. 30, No. 3, 11.2010, p. 446-51.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research

Harvard

Fritzsche, B, Schuchardt, J-P, Schmidt, A, Nau, H, Schweigert, FJ & Rühl, R 2010, 'CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats', REPROD TOXICOL, vol. 30, no. 3, pp. 446-51. https://doi.org/10.1016/j.reprotox.2010.05.005

APA

Fritzsche, B., Schuchardt, J-P., Schmidt, A., Nau, H., Schweigert, F. J., & Rühl, R. (2010). CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats. REPROD TOXICOL, 30(3), 446-51. https://doi.org/10.1016/j.reprotox.2010.05.005

Vancouver

Fritzsche B, Schuchardt J-P, Schmidt A, Nau H, Schweigert FJ, Rühl R. CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats. REPROD TOXICOL. 2010 Nov;30(3):446-51. https://doi.org/10.1016/j.reprotox.2010.05.005

Bibtex

@article{c915ec9bd7324864bbb53383b38b0495,
title = "CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats",
abstract = "Retinoids are essential in vertebrate reproduction and embryonic development. All-trans-retinoic acid (ATRA) is tightly regulated during these processes. CYP26A1 is mainly responsible for its degradation. To study the role of CYP26A1 during implantation, we applied R115866, a CYP26A1-specific antagonist, to rats during early gestation days (GD). On GD 6.5 and 12 samples were collected and the number of embryos was evaluated. ATRA concentration increased in uterus and serum, mRNA expression of CYP26A1 and CRABP2 increased in the liver, but not in the uterus. Uterine COX1 and 17βHSD mRNA expression was decreased. The number of embryos on GD 12 was not altered in this setting. It can be concluded that uterine expression of the analyzed retinoid-response genes during early gestation is not altered by this R115866 treatment and instead indirectly via ATRA. From our experiment we cannot confirm that ATRA obtains a major influencing role in the regulation of embryonic implantation.",
keywords = "Animals, Benzothiazoles, Body Weight, Chromatography, High Pressure Liquid, Cytochrome P-450 Enzyme Inhibitors, Cytochrome P-450 Enzyme System, Embryo Implantation, Female, Gene Expression, Liver, Organ Size, Pregnancy, Rats, Rats, Wistar, Retinoic Acid 4-Hydroxylase, Retinoids, Reverse Transcriptase Polymerase Chain Reaction, Triazoles, Uterus, Journal Article",
author = "Britta Fritzsche and Jan-Philipp Schuchardt and Anja Schmidt and Heinz Nau and Schweigert, {Florian J} and Ralph R{\"u}hl",
note = "Copyright {\textcopyright} 2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = nov,
doi = "10.1016/j.reprotox.2010.05.005",
language = "English",
volume = "30",
pages = "446--51",
journal = "REPROD TOXICOL",
issn = "0890-6238",
publisher = "Elsevier Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats

AU - Fritzsche, Britta

AU - Schuchardt, Jan-Philipp

AU - Schmidt, Anja

AU - Nau, Heinz

AU - Schweigert, Florian J

AU - Rühl, Ralph

N1 - Copyright © 2010 Elsevier Inc. All rights reserved.

PY - 2010/11

Y1 - 2010/11

N2 - Retinoids are essential in vertebrate reproduction and embryonic development. All-trans-retinoic acid (ATRA) is tightly regulated during these processes. CYP26A1 is mainly responsible for its degradation. To study the role of CYP26A1 during implantation, we applied R115866, a CYP26A1-specific antagonist, to rats during early gestation days (GD). On GD 6.5 and 12 samples were collected and the number of embryos was evaluated. ATRA concentration increased in uterus and serum, mRNA expression of CYP26A1 and CRABP2 increased in the liver, but not in the uterus. Uterine COX1 and 17βHSD mRNA expression was decreased. The number of embryos on GD 12 was not altered in this setting. It can be concluded that uterine expression of the analyzed retinoid-response genes during early gestation is not altered by this R115866 treatment and instead indirectly via ATRA. From our experiment we cannot confirm that ATRA obtains a major influencing role in the regulation of embryonic implantation.

AB - Retinoids are essential in vertebrate reproduction and embryonic development. All-trans-retinoic acid (ATRA) is tightly regulated during these processes. CYP26A1 is mainly responsible for its degradation. To study the role of CYP26A1 during implantation, we applied R115866, a CYP26A1-specific antagonist, to rats during early gestation days (GD). On GD 6.5 and 12 samples were collected and the number of embryos was evaluated. ATRA concentration increased in uterus and serum, mRNA expression of CYP26A1 and CRABP2 increased in the liver, but not in the uterus. Uterine COX1 and 17βHSD mRNA expression was decreased. The number of embryos on GD 12 was not altered in this setting. It can be concluded that uterine expression of the analyzed retinoid-response genes during early gestation is not altered by this R115866 treatment and instead indirectly via ATRA. From our experiment we cannot confirm that ATRA obtains a major influencing role in the regulation of embryonic implantation.

KW - Animals

KW - Benzothiazoles

KW - Body Weight

KW - Chromatography, High Pressure Liquid

KW - Cytochrome P-450 Enzyme Inhibitors

KW - Cytochrome P-450 Enzyme System

KW - Embryo Implantation

KW - Female

KW - Gene Expression

KW - Liver

KW - Organ Size

KW - Pregnancy

KW - Rats

KW - Rats, Wistar

KW - Retinoic Acid 4-Hydroxylase

KW - Retinoids

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Triazoles

KW - Uterus

KW - Journal Article

U2 - 10.1016/j.reprotox.2010.05.005

DO - 10.1016/j.reprotox.2010.05.005

M3 - SCORING: Journal article

C2 - 20580668

VL - 30

SP - 446

EP - 451

JO - REPROD TOXICOL

JF - REPROD TOXICOL

SN - 0890-6238

IS - 3

ER -