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Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients

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Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients. / Mitteldorf, Christina; Bertsch, Hans P; Zapf, Antonia; Neumann, Christine; Kretschmer, Lutz.

In: MODERN PATHOL, Vol. 22, No. 12, 12.2009, p. 1622-1627.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Mitteldorf, C, Bertsch, HP, Zapf, A, Neumann, C & Kretschmer, L 2009, 'Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients', MODERN PATHOL, vol. 22, no. 12, pp. 1622-1627. https://doi.org/10.1038/modpathol.2009.137

APA

Mitteldorf, C., Bertsch, H. P., Zapf, A., Neumann, C., & Kretschmer, L. (2009). Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients. MODERN PATHOL, 22(12), 1622-1627. https://doi.org/10.1038/modpathol.2009.137

Vancouver

Mitteldorf C, Bertsch HP, Zapf A, Neumann C, Kretschmer L. Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients. MODERN PATHOL. 2009 Dec;22(12):1622-1627. https://doi.org/10.1038/modpathol.2009.137

Bibtex

@article{55a361cd284946b0b9a160795037f26f,
title = "Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients",
abstract = "The optimal processing for the pathology of sentinel lymph nodes of patients with melanoma is still a matter of debate. We compared two protocols of sentinel lymph node processing, which were consecutively applied. For the first protocol, the sentinel lymph nodes were cut into 1-2 mm thick slices. From each slice, 12 microtome sections were stained (multiple slices protocol). For the second protocol, which is a modification of the recent European Organisation for Research and Treatment of Cancer protocol, the sentinel lymph nodes were bivalved. Five consecutive series of microtome sections, with gaps of 50 microm between them, were prepared from each cut surface (bivalving protocol). H&E and immunohistochemical staining were integral elements of both protocols. A total of 584 sentinel lymph nodes (1.8+/-0.9 per patient) were examined. The percentages of micrometastases (29 versus 27%) and of capsular naevi (13 versus 15%) detected were very similar for both protocols. As shown by multivariate logistic regression, Breslow thickness (P=0.003) and younger age (P=0.01) correlated with nodal metastasis. The type of histological preparation, ulceration and sex were not significant. The multiple slices protocol produced, on average, 4 paraffin blocks and 46 microtome sections per node. The bivalving protocol constantly produced 2 paraffin blocks and 42 microtome sections. For technical processing, the multiple slices protocol required, on average, 38 min per sentinel lymph node, whereas the bivalving protocol required 55 min. Both protocols yielded excellent detection rates with a similar amount of work being required on the part of the pathologist. Compared with the bivalving protocol, the multiple slices protocol was less labor intensive for the technical staff.",
keywords = "Adolescent, Adult, Aged, Aged, 80 and over, Coloring Agents, Eosine Yellowish-(YS), Female, Hematoxylin, Humans, Immunohistochemistry, Logistic Models, Lymphatic Metastasis, Male, Melanoma, Microtomy, Middle Aged, Paraffin Embedding, Predictive Value of Tests, Risk Assessment, Risk Factors, Sentinel Lymph Node Biopsy, Skin Neoplasms, Staining and Labeling, Time and Motion Studies, Workload, Young Adult, Comparative Study, Journal Article",
author = "Christina Mitteldorf and Bertsch, {Hans P} and Antonia Zapf and Christine Neumann and Lutz Kretschmer",
year = "2009",
month = dec,
doi = "10.1038/modpathol.2009.137",
language = "English",
volume = "22",
pages = "1622--1627",
journal = "MODERN PATHOL",
issn = "0893-3952",
publisher = "NATURE PUBLISHING GROUP",
number = "12",

}

RIS

TY - JOUR

T1 - Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients

AU - Mitteldorf, Christina

AU - Bertsch, Hans P

AU - Zapf, Antonia

AU - Neumann, Christine

AU - Kretschmer, Lutz

PY - 2009/12

Y1 - 2009/12

N2 - The optimal processing for the pathology of sentinel lymph nodes of patients with melanoma is still a matter of debate. We compared two protocols of sentinel lymph node processing, which were consecutively applied. For the first protocol, the sentinel lymph nodes were cut into 1-2 mm thick slices. From each slice, 12 microtome sections were stained (multiple slices protocol). For the second protocol, which is a modification of the recent European Organisation for Research and Treatment of Cancer protocol, the sentinel lymph nodes were bivalved. Five consecutive series of microtome sections, with gaps of 50 microm between them, were prepared from each cut surface (bivalving protocol). H&E and immunohistochemical staining were integral elements of both protocols. A total of 584 sentinel lymph nodes (1.8+/-0.9 per patient) were examined. The percentages of micrometastases (29 versus 27%) and of capsular naevi (13 versus 15%) detected were very similar for both protocols. As shown by multivariate logistic regression, Breslow thickness (P=0.003) and younger age (P=0.01) correlated with nodal metastasis. The type of histological preparation, ulceration and sex were not significant. The multiple slices protocol produced, on average, 4 paraffin blocks and 46 microtome sections per node. The bivalving protocol constantly produced 2 paraffin blocks and 42 microtome sections. For technical processing, the multiple slices protocol required, on average, 38 min per sentinel lymph node, whereas the bivalving protocol required 55 min. Both protocols yielded excellent detection rates with a similar amount of work being required on the part of the pathologist. Compared with the bivalving protocol, the multiple slices protocol was less labor intensive for the technical staff.

AB - The optimal processing for the pathology of sentinel lymph nodes of patients with melanoma is still a matter of debate. We compared two protocols of sentinel lymph node processing, which were consecutively applied. For the first protocol, the sentinel lymph nodes were cut into 1-2 mm thick slices. From each slice, 12 microtome sections were stained (multiple slices protocol). For the second protocol, which is a modification of the recent European Organisation for Research and Treatment of Cancer protocol, the sentinel lymph nodes were bivalved. Five consecutive series of microtome sections, with gaps of 50 microm between them, were prepared from each cut surface (bivalving protocol). H&E and immunohistochemical staining were integral elements of both protocols. A total of 584 sentinel lymph nodes (1.8+/-0.9 per patient) were examined. The percentages of micrometastases (29 versus 27%) and of capsular naevi (13 versus 15%) detected were very similar for both protocols. As shown by multivariate logistic regression, Breslow thickness (P=0.003) and younger age (P=0.01) correlated with nodal metastasis. The type of histological preparation, ulceration and sex were not significant. The multiple slices protocol produced, on average, 4 paraffin blocks and 46 microtome sections per node. The bivalving protocol constantly produced 2 paraffin blocks and 42 microtome sections. For technical processing, the multiple slices protocol required, on average, 38 min per sentinel lymph node, whereas the bivalving protocol required 55 min. Both protocols yielded excellent detection rates with a similar amount of work being required on the part of the pathologist. Compared with the bivalving protocol, the multiple slices protocol was less labor intensive for the technical staff.

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Coloring Agents

KW - Eosine Yellowish-(YS)

KW - Female

KW - Hematoxylin

KW - Humans

KW - Immunohistochemistry

KW - Logistic Models

KW - Lymphatic Metastasis

KW - Male

KW - Melanoma

KW - Microtomy

KW - Middle Aged

KW - Paraffin Embedding

KW - Predictive Value of Tests

KW - Risk Assessment

KW - Risk Factors

KW - Sentinel Lymph Node Biopsy

KW - Skin Neoplasms

KW - Staining and Labeling

KW - Time and Motion Studies

KW - Workload

KW - Young Adult

KW - Comparative Study

KW - Journal Article

U2 - 10.1038/modpathol.2009.137

DO - 10.1038/modpathol.2009.137

M3 - SCORING: Journal article

C2 - 19801968

VL - 22

SP - 1622

EP - 1627

JO - MODERN PATHOL

JF - MODERN PATHOL

SN - 0893-3952

IS - 12

ER -