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Course of HEV viremia and anti-HEV IgM/IgG response in asymptomatic blood donors

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Course of HEV viremia and anti-HEV IgM/IgG response in asymptomatic blood donors. / Kraef, Christian; Schlein, Christian; Hiller, Jens; Westhölter, Dirk; Denzer, Ulrike; Horvatits, Thomas; Peine, Sven; Lohse, Ansgar W; Lütgehetmann, Marc; Polywka, Susanne; Pischke, Sven.

In: J CLIN VIROL, Vol. 105, 08.2018, p. 26-30.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kraef, C, Schlein, C, Hiller, J, Westhölter, D, Denzer, U, Horvatits, T, Peine, S, Lohse, AW, Lütgehetmann, M, Polywka, S & Pischke, S 2018, 'Course of HEV viremia and anti-HEV IgM/IgG response in asymptomatic blood donors', J CLIN VIROL, vol. 105, pp. 26-30. https://doi.org/10.1016/j.jcv.2018.05.013

APA

Kraef, C., Schlein, C., Hiller, J., Westhölter, D., Denzer, U., Horvatits, T., Peine, S., Lohse, A. W., Lütgehetmann, M., Polywka, S., & Pischke, S. (2018). Course of HEV viremia and anti-HEV IgM/IgG response in asymptomatic blood donors. J CLIN VIROL, 105, 26-30. https://doi.org/10.1016/j.jcv.2018.05.013

Vancouver

Kraef C, Schlein C, Hiller J, Westhölter D, Denzer U, Horvatits T et al. Course of HEV viremia and anti-HEV IgM/IgG response in asymptomatic blood donors. J CLIN VIROL. 2018 Aug;105:26-30. https://doi.org/10.1016/j.jcv.2018.05.013

Bibtex

@article{ead6b61ab97449e3bcdf3542a25c29b5,
title = "Course of HEV viremia and anti-HEV IgM/IgG response in asymptomatic blood donors",
abstract = "BACKGROUND: Globally, an estimated 20 million Hepatitis E infections occur every year. The course of viremia and antibody response has been investigated in patients with symptomatic hepatitis E. However, the majority of HEV infections in industrialized countries take a subclinical course.OBJECTIVES: To investigate the course of HEV viremia and epitope specific anti-HEV IgM/IgG response in asymptomatic blood donors in order to understand the immune response and viral clearance in asymptomatic blood donors with HEV infections.METHODS: In this study 27 HEV viremic donors were identified by HEV-PCR during routine screening of blood donors and the course of anti-HEV IgM/IgG and HEV-RNA was retrospectively studied using RT-PCR and a commercial immunoblot (Mikrogen{\textregistered}) allowing classification of the antibody response according to HEV epitopes.RESULTS: At time of donation, serological testing failed to identify viremic donors as 70.4% had no detectable antibody response. Anti-HEV IgM could be detected in 22.2% of viremic donors while anti-HEV IgG could be found in 7.4%. At least three donors experienced prolonged viremia beyond 100 days. Spontaneous HEV-RNA clearance within a median time span of 57 days was observed in all 27 donors. In all donors anti-HEV IgG specific for the immunogenic viral epitope O2C could be detected in close temporal association with viral clearance.CONCLUSION: Serological testing is inappropriate for identifying HEV-viremic blood donors. Acute HEV infection in asymptomatic blood donors can persist for more than 100 days. HEV-RNA clearance coincided with the appearance of anti-HEV IgM/IgG confirming the importance of a B-cell mediated response in clearing acute infections. Anti-HEV IgM and IgG specific for the epitope O2C are associated with the clearance of HEV-viremia.",
keywords = "Journal Article",
author = "Christian Kraef and Christian Schlein and Jens Hiller and Dirk Westh{\"o}lter and Ulrike Denzer and Thomas Horvatits and Sven Peine and Lohse, {Ansgar W} and Marc L{\"u}tgehetmann and Susanne Polywka and Sven Pischke",
note = "Copyright {\textcopyright} 2018 Elsevier B.V. All rights reserved.",
year = "2018",
month = aug,
doi = "10.1016/j.jcv.2018.05.013",
language = "English",
volume = "105",
pages = "26--30",
journal = "J CLIN VIROL",
issn = "1386-6532",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Course of HEV viremia and anti-HEV IgM/IgG response in asymptomatic blood donors

AU - Kraef, Christian

AU - Schlein, Christian

AU - Hiller, Jens

AU - Westhölter, Dirk

AU - Denzer, Ulrike

AU - Horvatits, Thomas

AU - Peine, Sven

AU - Lohse, Ansgar W

AU - Lütgehetmann, Marc

AU - Polywka, Susanne

AU - Pischke, Sven

N1 - Copyright © 2018 Elsevier B.V. All rights reserved.

PY - 2018/8

Y1 - 2018/8

N2 - BACKGROUND: Globally, an estimated 20 million Hepatitis E infections occur every year. The course of viremia and antibody response has been investigated in patients with symptomatic hepatitis E. However, the majority of HEV infections in industrialized countries take a subclinical course.OBJECTIVES: To investigate the course of HEV viremia and epitope specific anti-HEV IgM/IgG response in asymptomatic blood donors in order to understand the immune response and viral clearance in asymptomatic blood donors with HEV infections.METHODS: In this study 27 HEV viremic donors were identified by HEV-PCR during routine screening of blood donors and the course of anti-HEV IgM/IgG and HEV-RNA was retrospectively studied using RT-PCR and a commercial immunoblot (Mikrogen®) allowing classification of the antibody response according to HEV epitopes.RESULTS: At time of donation, serological testing failed to identify viremic donors as 70.4% had no detectable antibody response. Anti-HEV IgM could be detected in 22.2% of viremic donors while anti-HEV IgG could be found in 7.4%. At least three donors experienced prolonged viremia beyond 100 days. Spontaneous HEV-RNA clearance within a median time span of 57 days was observed in all 27 donors. In all donors anti-HEV IgG specific for the immunogenic viral epitope O2C could be detected in close temporal association with viral clearance.CONCLUSION: Serological testing is inappropriate for identifying HEV-viremic blood donors. Acute HEV infection in asymptomatic blood donors can persist for more than 100 days. HEV-RNA clearance coincided with the appearance of anti-HEV IgM/IgG confirming the importance of a B-cell mediated response in clearing acute infections. Anti-HEV IgM and IgG specific for the epitope O2C are associated with the clearance of HEV-viremia.

AB - BACKGROUND: Globally, an estimated 20 million Hepatitis E infections occur every year. The course of viremia and antibody response has been investigated in patients with symptomatic hepatitis E. However, the majority of HEV infections in industrialized countries take a subclinical course.OBJECTIVES: To investigate the course of HEV viremia and epitope specific anti-HEV IgM/IgG response in asymptomatic blood donors in order to understand the immune response and viral clearance in asymptomatic blood donors with HEV infections.METHODS: In this study 27 HEV viremic donors were identified by HEV-PCR during routine screening of blood donors and the course of anti-HEV IgM/IgG and HEV-RNA was retrospectively studied using RT-PCR and a commercial immunoblot (Mikrogen®) allowing classification of the antibody response according to HEV epitopes.RESULTS: At time of donation, serological testing failed to identify viremic donors as 70.4% had no detectable antibody response. Anti-HEV IgM could be detected in 22.2% of viremic donors while anti-HEV IgG could be found in 7.4%. At least three donors experienced prolonged viremia beyond 100 days. Spontaneous HEV-RNA clearance within a median time span of 57 days was observed in all 27 donors. In all donors anti-HEV IgG specific for the immunogenic viral epitope O2C could be detected in close temporal association with viral clearance.CONCLUSION: Serological testing is inappropriate for identifying HEV-viremic blood donors. Acute HEV infection in asymptomatic blood donors can persist for more than 100 days. HEV-RNA clearance coincided with the appearance of anti-HEV IgM/IgG confirming the importance of a B-cell mediated response in clearing acute infections. Anti-HEV IgM and IgG specific for the epitope O2C are associated with the clearance of HEV-viremia.

KW - Journal Article

U2 - 10.1016/j.jcv.2018.05.013

DO - 10.1016/j.jcv.2018.05.013

M3 - SCORING: Journal article

C2 - 29860111

VL - 105

SP - 26

EP - 30

JO - J CLIN VIROL

JF - J CLIN VIROL

SN - 1386-6532

ER -