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Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity.

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Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity. / Ader, Christian; Schneider, Robert; Hornig, Sönke; Velisetty, Phanindra; Vardanyan, Vitya; Giller, Karin; Ohmert, Iris; Becker, Stefan; Pongs, Olaf; Baldus, Marc.

In: EMBO J, Vol. 28, No. 18, 18, 2009, p. 2825-2834.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ader, C, Schneider, R, Hornig, S, Velisetty, P, Vardanyan, V, Giller, K, Ohmert, I, Becker, S, Pongs, O & Baldus, M 2009, 'Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity.', EMBO J, vol. 28, no. 18, 18, pp. 2825-2834. <http://www.ncbi.nlm.nih.gov/pubmed/19661921?dopt=Citation>

APA

Ader, C., Schneider, R., Hornig, S., Velisetty, P., Vardanyan, V., Giller, K., Ohmert, I., Becker, S., Pongs, O., & Baldus, M. (2009). Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity. EMBO J, 28(18), 2825-2834. [18]. http://www.ncbi.nlm.nih.gov/pubmed/19661921?dopt=Citation

Vancouver

Ader C, Schneider R, Hornig S, Velisetty P, Vardanyan V, Giller K et al. Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity. EMBO J. 2009;28(18):2825-2834. 18.

Bibtex

@article{591d529eb2744d26a982693f8b295a5c,
title = "Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity.",
abstract = "Potassium (K(+))-channel gating is choreographed by a complex interplay between external stimuli, K(+) concentration and lipidic environment. We combined solid-state NMR and electrophysiological experiments on a chimeric KcsA-Kv1.3 channel to delineate K(+), pH and blocker effects on channel structure and function in a membrane setting. Our data show that pH-induced activation is correlated with protonation of glutamate residues at or near the activation gate. Moreover, K(+) and channel blockers distinctly affect the open probability of both the inactivation gate comprising the selectivity filter of the channel and the activation gate. The results indicate that the two gates are coupled and that effects of the permeant K(+) ion on the inactivation gate modulate activation-gate opening. Our data suggest a mechanism for controlling coordinated and sequential opening and closing of activation and inactivation gates in the K(+)-channel pore.",
author = "Christian Ader and Robert Schneider and S{\"o}nke Hornig and Phanindra Velisetty and Vitya Vardanyan and Karin Giller and Iris Ohmert and Stefan Becker and Olaf Pongs and Marc Baldus",
year = "2009",
language = "Deutsch",
volume = "28",
pages = "2825--2834",
journal = "EMBO J",
issn = "0261-4189",
publisher = "NATURE PUBLISHING GROUP",
number = "18",

}

RIS

TY - JOUR

T1 - Coupling of activation and inactivation gate in a K+-channel: potassium and ligand sensitivity.

AU - Ader, Christian

AU - Schneider, Robert

AU - Hornig, Sönke

AU - Velisetty, Phanindra

AU - Vardanyan, Vitya

AU - Giller, Karin

AU - Ohmert, Iris

AU - Becker, Stefan

AU - Pongs, Olaf

AU - Baldus, Marc

PY - 2009

Y1 - 2009

N2 - Potassium (K(+))-channel gating is choreographed by a complex interplay between external stimuli, K(+) concentration and lipidic environment. We combined solid-state NMR and electrophysiological experiments on a chimeric KcsA-Kv1.3 channel to delineate K(+), pH and blocker effects on channel structure and function in a membrane setting. Our data show that pH-induced activation is correlated with protonation of glutamate residues at or near the activation gate. Moreover, K(+) and channel blockers distinctly affect the open probability of both the inactivation gate comprising the selectivity filter of the channel and the activation gate. The results indicate that the two gates are coupled and that effects of the permeant K(+) ion on the inactivation gate modulate activation-gate opening. Our data suggest a mechanism for controlling coordinated and sequential opening and closing of activation and inactivation gates in the K(+)-channel pore.

AB - Potassium (K(+))-channel gating is choreographed by a complex interplay between external stimuli, K(+) concentration and lipidic environment. We combined solid-state NMR and electrophysiological experiments on a chimeric KcsA-Kv1.3 channel to delineate K(+), pH and blocker effects on channel structure and function in a membrane setting. Our data show that pH-induced activation is correlated with protonation of glutamate residues at or near the activation gate. Moreover, K(+) and channel blockers distinctly affect the open probability of both the inactivation gate comprising the selectivity filter of the channel and the activation gate. The results indicate that the two gates are coupled and that effects of the permeant K(+) ion on the inactivation gate modulate activation-gate opening. Our data suggest a mechanism for controlling coordinated and sequential opening and closing of activation and inactivation gates in the K(+)-channel pore.

M3 - SCORING: Zeitschriftenaufsatz

VL - 28

SP - 2825

EP - 2834

JO - EMBO J

JF - EMBO J

SN - 0261-4189

IS - 18

M1 - 18

ER -