Cotinine excretion (tobacco smoke biomarker) of smokers and non-smokers: comparison of GC/MS and RIA results.
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Cotinine excretion (tobacco smoke biomarker) of smokers and non-smokers: comparison of GC/MS and RIA results. / Heinrich-Ramm, Regine; Wegner, Ralf; Helene, Garde A; Baur, Xaver.
In: INT J HYG ENVIR HEAL, Vol. 205, No. 6, 6, 2002, p. 493-499.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Cotinine excretion (tobacco smoke biomarker) of smokers and non-smokers: comparison of GC/MS and RIA results.
AU - Heinrich-Ramm, Regine
AU - Wegner, Ralf
AU - Helene, Garde A
AU - Baur, Xaver
PY - 2002
Y1 - 2002
N2 - With a validated GC/MS method, the tobacco smoke biomarker cotinine has been estimated in urine for 148 non-smokers (male; 43 +/- 13 years; median 5.0 micrograms/g creatinine; 95th percentile 104 micrograms/g) and 96 smokers (male; 39 +/- 12 years; 1002 micrograms/g; 2993 micrograms/g). For a subgroup of 50 persons, the GC/MS results were compared with those by a commercially available radio immunoassay. Both methods identified the same persons as non-smokers and smokers, respectively, and were closely related. For smokers, the relationship was distinctly closer than for the non-smokers (r = 0.90, p <0.001, n = 14 vs. r = 0.41, p <0.02, n = 36). The RIA values were 2.4 times (smokers) and 2.9 times (non-smokers) higher than the GC/MS results. This was probably caused by the cross reactivity of the RIA antibodies against other urinary nicotine metabolites, e.g. trans-3'-hydroxycotinine, and has to be taken into account to correctly compare results of studies obtained with different analytical techniques and for choosing cut-off points to discriminate between active smokers and non-smokers of between non-smokers with higher or lower exposure to environmental tobacco smoke.
AB - With a validated GC/MS method, the tobacco smoke biomarker cotinine has been estimated in urine for 148 non-smokers (male; 43 +/- 13 years; median 5.0 micrograms/g creatinine; 95th percentile 104 micrograms/g) and 96 smokers (male; 39 +/- 12 years; 1002 micrograms/g; 2993 micrograms/g). For a subgroup of 50 persons, the GC/MS results were compared with those by a commercially available radio immunoassay. Both methods identified the same persons as non-smokers and smokers, respectively, and were closely related. For smokers, the relationship was distinctly closer than for the non-smokers (r = 0.90, p <0.001, n = 14 vs. r = 0.41, p <0.02, n = 36). The RIA values were 2.4 times (smokers) and 2.9 times (non-smokers) higher than the GC/MS results. This was probably caused by the cross reactivity of the RIA antibodies against other urinary nicotine metabolites, e.g. trans-3'-hydroxycotinine, and has to be taken into account to correctly compare results of studies obtained with different analytical techniques and for choosing cut-off points to discriminate between active smokers and non-smokers of between non-smokers with higher or lower exposure to environmental tobacco smoke.
M3 - SCORING: Zeitschriftenaufsatz
VL - 205
SP - 493
EP - 499
JO - INT J HYG ENVIR HEAL
JF - INT J HYG ENVIR HEAL
SN - 1438-4639
IS - 6
M1 - 6
ER -
