Co-replication analyses of naturally occurring defective hepatitis B virus variants with wild-type.
Standard
Co-replication analyses of naturally occurring defective hepatitis B virus variants with wild-type. / Märschenz, Stefanie; Brinckmann, Anja; Nürnberg, Peter; Krüger, Detlev; Günther, Stephan; Meisel, Helga.
In: VIROLOGY, Vol. 372, No. 2, 2, 2008, p. 247-259.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Co-replication analyses of naturally occurring defective hepatitis B virus variants with wild-type.
AU - Märschenz, Stefanie
AU - Brinckmann, Anja
AU - Nürnberg, Peter
AU - Krüger, Detlev
AU - Günther, Stephan
AU - Meisel, Helga
PY - 2008
Y1 - 2008
N2 - Complex hepatitis B virus (HBV) variants with mutations in core promoter (Cp) plus deletions in the C gene and/or preS region--that are associated with development of liver cirrhosis in renal transplant recipients--show a drastically changed phenotype with altered transcription and disturbed surface and core protein expression. Here, we analyzed the replication phenotype of six different defective variant genomes, isolated from two patients, after co-transfection with HBV wild-type (wt) in varying proportions. Both in HuH7 and HepG2 cells, the variants showed enhanced replication and enrichment in the different transfected variant-wt mixtures. Contrary to artificial variants with only C gene deletions in wt context, the original complex variants as well as wt genomes carrying C gene and Cp mutations of the variants did mostly not suppress wt replication. Thus, the Cp mutations compensate the suppression of wt by C gene deletions and furthermore enhance the replication level.
AB - Complex hepatitis B virus (HBV) variants with mutations in core promoter (Cp) plus deletions in the C gene and/or preS region--that are associated with development of liver cirrhosis in renal transplant recipients--show a drastically changed phenotype with altered transcription and disturbed surface and core protein expression. Here, we analyzed the replication phenotype of six different defective variant genomes, isolated from two patients, after co-transfection with HBV wild-type (wt) in varying proportions. Both in HuH7 and HepG2 cells, the variants showed enhanced replication and enrichment in the different transfected variant-wt mixtures. Contrary to artificial variants with only C gene deletions in wt context, the original complex variants as well as wt genomes carrying C gene and Cp mutations of the variants did mostly not suppress wt replication. Thus, the Cp mutations compensate the suppression of wt by C gene deletions and furthermore enhance the replication level.
M3 - SCORING: Zeitschriftenaufsatz
VL - 372
SP - 247
EP - 259
JO - VIROLOGY
JF - VIROLOGY
SN - 0042-6822
IS - 2
M1 - 2
ER -
