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Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs

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Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. / Ohl, Lars; Henning, Golo; Krautwald, Stefan; Lipp, Martin; Hardtke, Svenja; Bernhardt, Gunter; Pabst, Oliver; Förster, Reinhold.

In: J EXP MED, Vol. 197, No. 9, 05.05.2003, p. 1199-204.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ohl, L, Henning, G, Krautwald, S, Lipp, M, Hardtke, S, Bernhardt, G, Pabst, O & Förster, R 2003, 'Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs', J EXP MED, vol. 197, no. 9, pp. 1199-204. https://doi.org/10.1084/jem.20030169

APA

Ohl, L., Henning, G., Krautwald, S., Lipp, M., Hardtke, S., Bernhardt, G., Pabst, O., & Förster, R. (2003). Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. J EXP MED, 197(9), 1199-204. https://doi.org/10.1084/jem.20030169

Vancouver

Ohl L, Henning G, Krautwald S, Lipp M, Hardtke S, Bernhardt G et al. Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. J EXP MED. 2003 May 5;197(9):1199-204. https://doi.org/10.1084/jem.20030169

Bibtex

@article{75dcd88021c140e28c3c938ba8e98bae,
title = "Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs",
abstract = "Homeostatic chemokines participate in the development of secondary lymphoid organs and later on in the functional organization of these tissues. The development of lymph nodes (LNs) and Peyer's patches depends on the recruitment of CD3- CD4+ interleukin (IL)-7R alpha hi cells to sites of future organ development. CD3- CD4+ IL-7R alpha hi cells express the chemokine receptor CXCR5 and might be attracted by its ligand CXCL13, which is secreted by mesenchymal cells. Mesenchymal cells also secrete CCL19, a ligand for CCR7, yet it is not clear whether CCR7 and CCL19 are important for secondary lymphoid organ development. Analyzing CXCR5-/- CCR7-/- double deficient mice we now show that these mice lack all examined peripheral LNs suggesting a profound role for both receptors in secondary lymphoid organ development. We demonstrate that CD3- CD4+ IL-7R alpha hi cells express CXCR5 as well as CCR7 indicating that both receptors cooperate during an early step of secondary lymphoid organ development. Furthermore, CXCR5-/- CCR7-/- mice display a severely disturbed architecture of mesenteric LN and spleen. Due to an impaired migration of B cells into the white pulp, CXCR5-/- CCR7-/- mice fail to develop B cell follicles but show small clusters of unorganized lymphocytes in the spleen. These data demonstrate a cooperative function of CXCR5 and CCR7 in lymphoid organ organogenesis and organization.",
keywords = "Animals, Immunophenotyping, Lymphoid Tissue/growth & development, Mice, Mice, Knockout, Receptors, CCR7, Receptors, CXCR5, Receptors, Chemokine/genetics, Receptors, Cytokine/genetics",
author = "Lars Ohl and Golo Henning and Stefan Krautwald and Martin Lipp and Svenja Hardtke and Gunter Bernhardt and Oliver Pabst and Reinhold F{\"o}rster",
year = "2003",
month = may,
day = "5",
doi = "10.1084/jem.20030169",
language = "English",
volume = "197",
pages = "1199--204",
journal = "J EXP MED",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "9",

}

RIS

TY - JOUR

T1 - Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs

AU - Ohl, Lars

AU - Henning, Golo

AU - Krautwald, Stefan

AU - Lipp, Martin

AU - Hardtke, Svenja

AU - Bernhardt, Gunter

AU - Pabst, Oliver

AU - Förster, Reinhold

PY - 2003/5/5

Y1 - 2003/5/5

N2 - Homeostatic chemokines participate in the development of secondary lymphoid organs and later on in the functional organization of these tissues. The development of lymph nodes (LNs) and Peyer's patches depends on the recruitment of CD3- CD4+ interleukin (IL)-7R alpha hi cells to sites of future organ development. CD3- CD4+ IL-7R alpha hi cells express the chemokine receptor CXCR5 and might be attracted by its ligand CXCL13, which is secreted by mesenchymal cells. Mesenchymal cells also secrete CCL19, a ligand for CCR7, yet it is not clear whether CCR7 and CCL19 are important for secondary lymphoid organ development. Analyzing CXCR5-/- CCR7-/- double deficient mice we now show that these mice lack all examined peripheral LNs suggesting a profound role for both receptors in secondary lymphoid organ development. We demonstrate that CD3- CD4+ IL-7R alpha hi cells express CXCR5 as well as CCR7 indicating that both receptors cooperate during an early step of secondary lymphoid organ development. Furthermore, CXCR5-/- CCR7-/- mice display a severely disturbed architecture of mesenteric LN and spleen. Due to an impaired migration of B cells into the white pulp, CXCR5-/- CCR7-/- mice fail to develop B cell follicles but show small clusters of unorganized lymphocytes in the spleen. These data demonstrate a cooperative function of CXCR5 and CCR7 in lymphoid organ organogenesis and organization.

AB - Homeostatic chemokines participate in the development of secondary lymphoid organs and later on in the functional organization of these tissues. The development of lymph nodes (LNs) and Peyer's patches depends on the recruitment of CD3- CD4+ interleukin (IL)-7R alpha hi cells to sites of future organ development. CD3- CD4+ IL-7R alpha hi cells express the chemokine receptor CXCR5 and might be attracted by its ligand CXCL13, which is secreted by mesenchymal cells. Mesenchymal cells also secrete CCL19, a ligand for CCR7, yet it is not clear whether CCR7 and CCL19 are important for secondary lymphoid organ development. Analyzing CXCR5-/- CCR7-/- double deficient mice we now show that these mice lack all examined peripheral LNs suggesting a profound role for both receptors in secondary lymphoid organ development. We demonstrate that CD3- CD4+ IL-7R alpha hi cells express CXCR5 as well as CCR7 indicating that both receptors cooperate during an early step of secondary lymphoid organ development. Furthermore, CXCR5-/- CCR7-/- mice display a severely disturbed architecture of mesenteric LN and spleen. Due to an impaired migration of B cells into the white pulp, CXCR5-/- CCR7-/- mice fail to develop B cell follicles but show small clusters of unorganized lymphocytes in the spleen. These data demonstrate a cooperative function of CXCR5 and CCR7 in lymphoid organ organogenesis and organization.

KW - Animals

KW - Immunophenotyping

KW - Lymphoid Tissue/growth & development

KW - Mice

KW - Mice, Knockout

KW - Receptors, CCR7

KW - Receptors, CXCR5

KW - Receptors, Chemokine/genetics

KW - Receptors, Cytokine/genetics

U2 - 10.1084/jem.20030169

DO - 10.1084/jem.20030169

M3 - SCORING: Journal article

C2 - 12732661

VL - 197

SP - 1199

EP - 1204

JO - J EXP MED

JF - J EXP MED

SN - 0022-1007

IS - 9

ER -