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Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury

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Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury. / Stüdemann, Tim; Rössinger, Judith; Manthey, Christoph; Geertz, Birgit; Srikantharajah, Rajiven; von Bibra, Constantin; Shibamiya, Aya; Köhne, Maria; Wiehler, Antonius; Wiegert, J Simon; Eschenhagen, Thomas; Weinberger, Florian.

In: CIRCULATION, Vol. 146, No. 15, 11.10.2022, p. 1159-1169.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Stüdemann, T, Rössinger, J, Manthey, C, Geertz, B, Srikantharajah, R, von Bibra, C, Shibamiya, A, Köhne, M, Wiehler, A, Wiegert, JS, Eschenhagen, T & Weinberger, F 2022, 'Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury', CIRCULATION, vol. 146, no. 15, pp. 1159-1169. https://doi.org/10.1161/CIRCULATIONAHA.122.060124

APA

Stüdemann, T., Rössinger, J., Manthey, C., Geertz, B., Srikantharajah, R., von Bibra, C., Shibamiya, A., Köhne, M., Wiehler, A., Wiegert, J. S., Eschenhagen, T., & Weinberger, F. (2022). Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury. CIRCULATION, 146(15), 1159-1169. https://doi.org/10.1161/CIRCULATIONAHA.122.060124

Vancouver

Stüdemann T, Rössinger J, Manthey C, Geertz B, Srikantharajah R, von Bibra C et al. Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury. CIRCULATION. 2022 Oct 11;146(15):1159-1169. https://doi.org/10.1161/CIRCULATIONAHA.122.060124

Bibtex

@article{bb49dafc7adb4c7cad3c69af819a2bbd,
title = "Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury",
abstract = "BACKGROUND: Transplantation of pluripotent stem cell-derived cardiomyocytes represents a promising therapeutic strategy for cardiac regeneration, and the first clinical studies in patients with heart failure have commenced. Yet, little is known about the mechanism of action underlying graft-induced benefits. Here, we explored whether transplanted cardiomyocytes actively contribute to heart function.METHODS: We injected cardiomyocytes with an optogenetic off-on switch in a guinea pig cardiac injury model.RESULTS: Light-induced inhibition of engrafted cardiomyocyte contractility resulted in a rapid decrease of left ventricular function in ≈50% (7/13) animals that was fully reversible with the offset of photostimulation.CONCLUSIONS: Our optogenetic approach demonstrates that transplanted cardiomyocytes can actively participate in heart function, supporting the hypothesis that the delivery of new force-generating myocardium can serve as a regenerative therapeutic strategy.",
keywords = "Animals, Cell Differentiation/physiology, Guinea Pigs, Myocardium, Myocytes, Cardiac/transplantation, Pluripotent Stem Cells/physiology, Ventricular Function, Left",
author = "Tim St{\"u}demann and Judith R{\"o}ssinger and Christoph Manthey and Birgit Geertz and Rajiven Srikantharajah and {von Bibra}, Constantin and Aya Shibamiya and Maria K{\"o}hne and Antonius Wiehler and Wiegert, {J Simon} and Thomas Eschenhagen and Florian Weinberger",
year = "2022",
month = oct,
day = "11",
doi = "10.1161/CIRCULATIONAHA.122.060124",
language = "English",
volume = "146",
pages = "1159--1169",
journal = "CIRCULATION",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "15",

}

RIS

TY - JOUR

T1 - Contractile Force of Transplanted Cardiomyocytes Actively Supports Heart Function After Injury

AU - Stüdemann, Tim

AU - Rössinger, Judith

AU - Manthey, Christoph

AU - Geertz, Birgit

AU - Srikantharajah, Rajiven

AU - von Bibra, Constantin

AU - Shibamiya, Aya

AU - Köhne, Maria

AU - Wiehler, Antonius

AU - Wiegert, J Simon

AU - Eschenhagen, Thomas

AU - Weinberger, Florian

PY - 2022/10/11

Y1 - 2022/10/11

N2 - BACKGROUND: Transplantation of pluripotent stem cell-derived cardiomyocytes represents a promising therapeutic strategy for cardiac regeneration, and the first clinical studies in patients with heart failure have commenced. Yet, little is known about the mechanism of action underlying graft-induced benefits. Here, we explored whether transplanted cardiomyocytes actively contribute to heart function.METHODS: We injected cardiomyocytes with an optogenetic off-on switch in a guinea pig cardiac injury model.RESULTS: Light-induced inhibition of engrafted cardiomyocyte contractility resulted in a rapid decrease of left ventricular function in ≈50% (7/13) animals that was fully reversible with the offset of photostimulation.CONCLUSIONS: Our optogenetic approach demonstrates that transplanted cardiomyocytes can actively participate in heart function, supporting the hypothesis that the delivery of new force-generating myocardium can serve as a regenerative therapeutic strategy.

AB - BACKGROUND: Transplantation of pluripotent stem cell-derived cardiomyocytes represents a promising therapeutic strategy for cardiac regeneration, and the first clinical studies in patients with heart failure have commenced. Yet, little is known about the mechanism of action underlying graft-induced benefits. Here, we explored whether transplanted cardiomyocytes actively contribute to heart function.METHODS: We injected cardiomyocytes with an optogenetic off-on switch in a guinea pig cardiac injury model.RESULTS: Light-induced inhibition of engrafted cardiomyocyte contractility resulted in a rapid decrease of left ventricular function in ≈50% (7/13) animals that was fully reversible with the offset of photostimulation.CONCLUSIONS: Our optogenetic approach demonstrates that transplanted cardiomyocytes can actively participate in heart function, supporting the hypothesis that the delivery of new force-generating myocardium can serve as a regenerative therapeutic strategy.

KW - Animals

KW - Cell Differentiation/physiology

KW - Guinea Pigs

KW - Myocardium

KW - Myocytes, Cardiac/transplantation

KW - Pluripotent Stem Cells/physiology

KW - Ventricular Function, Left

U2 - 10.1161/CIRCULATIONAHA.122.060124

DO - 10.1161/CIRCULATIONAHA.122.060124

M3 - SCORING: Journal article

C2 - 36073365

VL - 146

SP - 1159

EP - 1169

JO - CIRCULATION

JF - CIRCULATION

SN - 0009-7322

IS - 15

ER -