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Content of Asthmagen Natural Rubber Latex Allergens in Commercial Disposable Gloves

Standard

Content of Asthmagen Natural Rubber Latex Allergens in Commercial Disposable Gloves. / Bittner, C; Velasco Garrido, Marcial; Krach, L H ; Harth, V.

In: ADV EXP MED BIOL, Vol. 921, 31.05.2016, p. 37-44.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bittner, C, Velasco Garrido, M, Krach, LH & Harth, V 2016, 'Content of Asthmagen Natural Rubber Latex Allergens in Commercial Disposable Gloves', ADV EXP MED BIOL, vol. 921, pp. 37-44. https://doi.org/10.1007/5584_2016_227

APA

Bittner, C., Velasco Garrido, M., Krach, L. H., & Harth, V. (2016). Content of Asthmagen Natural Rubber Latex Allergens in Commercial Disposable Gloves. ADV EXP MED BIOL, 921, 37-44. https://doi.org/10.1007/5584_2016_227

Vancouver

Bittner C, Velasco Garrido M, Krach LH, Harth V. Content of Asthmagen Natural Rubber Latex Allergens in Commercial Disposable Gloves. ADV EXP MED BIOL. 2016 May 31;921:37-44. https://doi.org/10.1007/5584_2016_227

Bibtex

@article{744d3a8b0dc84f9286d61dea26212a30,
title = "Content of Asthmagen Natural Rubber Latex Allergens in Commercial Disposable Gloves",
abstract = "The use of natural rubber latex (NRL) gloves in many occupations may lead to latex sensitization, allergic asthma, and skin reactions. Due to their good properties and environmental safety NRL gloves are still being used in the healthcare setting, but also in the food industry, by hairdressers, cleaners, etc. The aim of our study was to assess the protein and NRL allergen content in commercial gloves by different methods, including a new assay. Twenty commercially available NRL gloves were analyzed. Protein extraction was performed according to the international standard ASTM D-5712. Total protein content was measured with a modified Lowry method, NRL content with the CAP Inhibition Assay, the Beezhold ELISA Inhibition Assay, and an innovative ELISA with IgY-antibodies extracted from eggs of NRL-immunized hens (IgY Inhibition Assay). We found a high protein content in a range of 215.0-1304.7 μg/g in 8 out of the 20 NRL gloves. Seven of the 20 gloves were powdered, four of them with a high protein content. In gloves with high protein content, the immunological tests detected congruently high levels of NRL allergen. We conclude that a high percentage of commercially available NRL gloves still represent a risk for NRL allergy, including asthma. The modified Lowry Method allows to infer on the latex allergen content.",
author = "C Bittner and {Velasco Garrido}, Marcial and Krach, {L H} and V Harth",
year = "2016",
month = may,
day = "31",
doi = "10.1007/5584_2016_227",
language = "English",
volume = "921",
pages = "37--44",
journal = "ADV EXP MED BIOL",
issn = "0065-2598",
publisher = "Springer New York",

}

RIS

TY - JOUR

T1 - Content of Asthmagen Natural Rubber Latex Allergens in Commercial Disposable Gloves

AU - Bittner, C

AU - Velasco Garrido, Marcial

AU - Krach, L H

AU - Harth, V

PY - 2016/5/31

Y1 - 2016/5/31

N2 - The use of natural rubber latex (NRL) gloves in many occupations may lead to latex sensitization, allergic asthma, and skin reactions. Due to their good properties and environmental safety NRL gloves are still being used in the healthcare setting, but also in the food industry, by hairdressers, cleaners, etc. The aim of our study was to assess the protein and NRL allergen content in commercial gloves by different methods, including a new assay. Twenty commercially available NRL gloves were analyzed. Protein extraction was performed according to the international standard ASTM D-5712. Total protein content was measured with a modified Lowry method, NRL content with the CAP Inhibition Assay, the Beezhold ELISA Inhibition Assay, and an innovative ELISA with IgY-antibodies extracted from eggs of NRL-immunized hens (IgY Inhibition Assay). We found a high protein content in a range of 215.0-1304.7 μg/g in 8 out of the 20 NRL gloves. Seven of the 20 gloves were powdered, four of them with a high protein content. In gloves with high protein content, the immunological tests detected congruently high levels of NRL allergen. We conclude that a high percentage of commercially available NRL gloves still represent a risk for NRL allergy, including asthma. The modified Lowry Method allows to infer on the latex allergen content.

AB - The use of natural rubber latex (NRL) gloves in many occupations may lead to latex sensitization, allergic asthma, and skin reactions. Due to their good properties and environmental safety NRL gloves are still being used in the healthcare setting, but also in the food industry, by hairdressers, cleaners, etc. The aim of our study was to assess the protein and NRL allergen content in commercial gloves by different methods, including a new assay. Twenty commercially available NRL gloves were analyzed. Protein extraction was performed according to the international standard ASTM D-5712. Total protein content was measured with a modified Lowry method, NRL content with the CAP Inhibition Assay, the Beezhold ELISA Inhibition Assay, and an innovative ELISA with IgY-antibodies extracted from eggs of NRL-immunized hens (IgY Inhibition Assay). We found a high protein content in a range of 215.0-1304.7 μg/g in 8 out of the 20 NRL gloves. Seven of the 20 gloves were powdered, four of them with a high protein content. In gloves with high protein content, the immunological tests detected congruently high levels of NRL allergen. We conclude that a high percentage of commercially available NRL gloves still represent a risk for NRL allergy, including asthma. The modified Lowry Method allows to infer on the latex allergen content.

U2 - 10.1007/5584_2016_227

DO - 10.1007/5584_2016_227

M3 - SCORING: Journal article

C2 - 27241513

VL - 921

SP - 37

EP - 44

JO - ADV EXP MED BIOL

JF - ADV EXP MED BIOL

SN - 0065-2598

ER -