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Constitutive expression and regulated release of the transmembrane chemokine CXCL16 in human and murine skin

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Constitutive expression and regulated release of the transmembrane chemokine CXCL16 in human and murine skin. / Scholz, Felix; Schulte, Alexander; Adamski, Frederic; Hundhausen, Christian; Mittag, Jens; Schwarz, Agatha; Kruse, Marie-Luise; Proksch, Ehrhardt; Ludwig, Andreas.

In: J INVEST DERMATOL, Vol. 127, No. 6, 01.06.2007, p. 1444-55.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Scholz, F, Schulte, A, Adamski, F, Hundhausen, C, Mittag, J, Schwarz, A, Kruse, M-L, Proksch, E & Ludwig, A 2007, 'Constitutive expression and regulated release of the transmembrane chemokine CXCL16 in human and murine skin', J INVEST DERMATOL, vol. 127, no. 6, pp. 1444-55. https://doi.org/10.1038/sj.jid.5700751

APA

Scholz, F., Schulte, A., Adamski, F., Hundhausen, C., Mittag, J., Schwarz, A., Kruse, M-L., Proksch, E., & Ludwig, A. (2007). Constitutive expression and regulated release of the transmembrane chemokine CXCL16 in human and murine skin. J INVEST DERMATOL, 127(6), 1444-55. https://doi.org/10.1038/sj.jid.5700751

Vancouver

Scholz F, Schulte A, Adamski F, Hundhausen C, Mittag J, Schwarz A et al. Constitutive expression and regulated release of the transmembrane chemokine CXCL16 in human and murine skin. J INVEST DERMATOL. 2007 Jun 1;127(6):1444-55. https://doi.org/10.1038/sj.jid.5700751

Bibtex

@article{65a7165ac29f41ecae1b47d4a68a9454,
title = "Constitutive expression and regulated release of the transmembrane chemokine CXCL16 in human and murine skin",
abstract = "The CXC-chemokine ligand 16 (CXCL16) is expressed as a transmembrane adhesion molecule and can be released as a chemoattractant. Both functions are carried out by binding of CXCL16 to its receptor, CXC-chemokine receptor 6 (CXCR6). We here provide early evidence that CXCL16 is expressed in situ by epidermal keratinocytes of normal skin on messenger RNA and protein level and released into the wound exudate upon injury. Cultured human and murine keratinocyte cell lines (HaCaT and PAM212, respectively) as well as primary keratinocyte cultures constitutively express transmembrane CXCL16 on the cell surface. Soluble CXCL16 is released by its limited proteolytic cleavage involving the disintegrin-like metalloproteinase (ADAM)10 but not the closely related ADAM17, as shown by specific inhibitors and small-interfering RNA knockdown experiments. This shedding of CXCL16 is reduced by serum starvation but enhanced by cell stimulation with ionomycin or by UVB irradiation. Soluble CXCL16 from keratinocytes was shown to bind and activate CXCR6, and marked expression of this receptor was found on a subpopulation of T cells in the dermis. Thus, CXCL16 is constitutively expressed on the surface of human epidermal keratinocytes, released upon cell activation or photodamage and may then target CXCR6-expressing T cells in the dermis.",
keywords = "ADAM Proteins, Amyloid Precursor Protein Secretases, Animals, Cell Adhesion, Cell Line, Chemokines, CXC, Dermis, Epidermis, Gene Expression, Humans, Keratinocytes, Kidney, Membrane Proteins, Mice, Mice, Hairless, RNA, Small Interfering, Receptors, Scavenger, Skin, Skin Aging, Solubility, Up-Regulation",
author = "Felix Scholz and Alexander Schulte and Frederic Adamski and Christian Hundhausen and Jens Mittag and Agatha Schwarz and Marie-Luise Kruse and Ehrhardt Proksch and Andreas Ludwig",
year = "2007",
month = jun,
day = "1",
doi = "10.1038/sj.jid.5700751",
language = "English",
volume = "127",
pages = "1444--55",
journal = "J INVEST DERMATOL",
issn = "0022-202X",
publisher = "NATURE PUBLISHING GROUP",
number = "6",

}

RIS

TY - JOUR

T1 - Constitutive expression and regulated release of the transmembrane chemokine CXCL16 in human and murine skin

AU - Scholz, Felix

AU - Schulte, Alexander

AU - Adamski, Frederic

AU - Hundhausen, Christian

AU - Mittag, Jens

AU - Schwarz, Agatha

AU - Kruse, Marie-Luise

AU - Proksch, Ehrhardt

AU - Ludwig, Andreas

PY - 2007/6/1

Y1 - 2007/6/1

N2 - The CXC-chemokine ligand 16 (CXCL16) is expressed as a transmembrane adhesion molecule and can be released as a chemoattractant. Both functions are carried out by binding of CXCL16 to its receptor, CXC-chemokine receptor 6 (CXCR6). We here provide early evidence that CXCL16 is expressed in situ by epidermal keratinocytes of normal skin on messenger RNA and protein level and released into the wound exudate upon injury. Cultured human and murine keratinocyte cell lines (HaCaT and PAM212, respectively) as well as primary keratinocyte cultures constitutively express transmembrane CXCL16 on the cell surface. Soluble CXCL16 is released by its limited proteolytic cleavage involving the disintegrin-like metalloproteinase (ADAM)10 but not the closely related ADAM17, as shown by specific inhibitors and small-interfering RNA knockdown experiments. This shedding of CXCL16 is reduced by serum starvation but enhanced by cell stimulation with ionomycin or by UVB irradiation. Soluble CXCL16 from keratinocytes was shown to bind and activate CXCR6, and marked expression of this receptor was found on a subpopulation of T cells in the dermis. Thus, CXCL16 is constitutively expressed on the surface of human epidermal keratinocytes, released upon cell activation or photodamage and may then target CXCR6-expressing T cells in the dermis.

AB - The CXC-chemokine ligand 16 (CXCL16) is expressed as a transmembrane adhesion molecule and can be released as a chemoattractant. Both functions are carried out by binding of CXCL16 to its receptor, CXC-chemokine receptor 6 (CXCR6). We here provide early evidence that CXCL16 is expressed in situ by epidermal keratinocytes of normal skin on messenger RNA and protein level and released into the wound exudate upon injury. Cultured human and murine keratinocyte cell lines (HaCaT and PAM212, respectively) as well as primary keratinocyte cultures constitutively express transmembrane CXCL16 on the cell surface. Soluble CXCL16 is released by its limited proteolytic cleavage involving the disintegrin-like metalloproteinase (ADAM)10 but not the closely related ADAM17, as shown by specific inhibitors and small-interfering RNA knockdown experiments. This shedding of CXCL16 is reduced by serum starvation but enhanced by cell stimulation with ionomycin or by UVB irradiation. Soluble CXCL16 from keratinocytes was shown to bind and activate CXCR6, and marked expression of this receptor was found on a subpopulation of T cells in the dermis. Thus, CXCL16 is constitutively expressed on the surface of human epidermal keratinocytes, released upon cell activation or photodamage and may then target CXCR6-expressing T cells in the dermis.

KW - ADAM Proteins

KW - Amyloid Precursor Protein Secretases

KW - Animals

KW - Cell Adhesion

KW - Cell Line

KW - Chemokines, CXC

KW - Dermis

KW - Epidermis

KW - Gene Expression

KW - Humans

KW - Keratinocytes

KW - Kidney

KW - Membrane Proteins

KW - Mice

KW - Mice, Hairless

KW - RNA, Small Interfering

KW - Receptors, Scavenger

KW - Skin

KW - Skin Aging

KW - Solubility

KW - Up-Regulation

U2 - 10.1038/sj.jid.5700751

DO - 10.1038/sj.jid.5700751

M3 - SCORING: Journal article

C2 - 17363916

VL - 127

SP - 1444

EP - 1455

JO - J INVEST DERMATOL

JF - J INVEST DERMATOL

SN - 0022-202X

IS - 6

ER -