Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis

Malte Rühlemann; Timur Liwinski; Femke-Anouska Heinsen; Corinna Bang; Roman Zenouzi; Martin Kummen; Louise Thingholm; Marie Tempel; Wolfgang Lieb; Tom Karlsen; Ansgar Lohse; Johannes Hov; Gerald Denk; Frank Lammert; Marcin Krawczyk; Christoph Schramm; Andre Franke

doi:10.1111/apt.15375

Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis

Standard

Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis. / Rühlemann, Malte; Liwinski, Timur; Heinsen, Femke-Anouska; Bang, Corinna; Zenouzi, Roman; Kummen, Martin; Thingholm, Louise; Tempel, Marie; Lieb, Wolfgang; Karlsen, Tom; Lohse, Ansgar; Hov, Johannes; Denk, Gerald; Lammert, Frank; Krawczyk, Marcin; Schramm, Christoph; Franke, Andre.

In: ALIMENT PHARM THER, Vol. 50, No. 5, 09.2019, p. 580-589.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rühlemann, M, Liwinski, T, Heinsen, F-A, Bang, C, Zenouzi, R, Kummen, M, Thingholm, L, Tempel, M, Lieb, W, Karlsen, T, Lohse, A, Hov, J, Denk, G, Lammert, F, Krawczyk, M, Schramm, C & Franke, A 2019, 'Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis', ALIMENT PHARM THER, vol. 50, no. 5, pp. 580-589. https://doi.org/10.1111/apt.15375

APA

Rühlemann, M., Liwinski, T., Heinsen, F-A., Bang, C., Zenouzi, R., Kummen, M., Thingholm, L., Tempel, M., Lieb, W., Karlsen, T., Lohse, A., Hov, J., Denk, G., Lammert, F., Krawczyk, M., Schramm, C., & Franke, A. (2019). Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis. ALIMENT PHARM THER, 50(5), 580-589. https://doi.org/10.1111/apt.15375

Vancouver

Rühlemann M, Liwinski T, Heinsen F-A, Bang C, Zenouzi R, Kummen M et al. Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis. ALIMENT PHARM THER. 2019 Sep;50(5):580-589. https://doi.org/10.1111/apt.15375

Bibtex

@article{fe7bd24b0a1b46c89da7796b0d1e34a5,

title = "Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis",

abstract = "BACKGROUND: Single-centre studies reported alterations of faecal microbiota in patients with primary sclerosing cholangitis (PSC). As regional factors may affect microbial communities, it is unclear if a microbial signature of PSC exists across different geographical regions.AIM: To identify a robust microbial signature of PSC independent of geography and environmental influences.METHODS: We included 388 individuals (median age, 47 years; range, 15-78) from Germany and Norway in the study, 137 patients with PSC (n = 75 with colitis), 118 with ulcerative colitis (UC) and 133 healthy controls. Faecal microbiomes were analysed by 16S rRNA gene sequencing (V1-V2). Differences in relative abundances of single taxa were subjected to a meta-analysis.RESULTS: In both cohorts, microbiota composition (beta-diversity) differed between PSC patients and controls (P < 0.001). Random forests classification discriminated PSC patients from controls in both geographical cohorts with an average area under the curve of 0.88. Compared to healthy controls, many new cohort-spanning alterations were identified in PSC, such as an increase of Proteobacteria and the bile-tolerant genus Parabacteroides, which were detected independent from geographical region. Associated colitis only had minor effects on microbiota composition, suggesting that PSC itself drives the faecal microbiota changes observed.CONCLUSION: Compared to healthy controls, numerous microbiota alterations are reproducible in PSC patients across geographical regions, clearly pointing towards a microbiota composition that is shaped by the disease itself and not by environmental factors. These reproducibly altered microbial populations might provide future insights into the pathophysiology of PSC.",

author = "Malte R{\"u}hlemann and Timur Liwinski and Femke-Anouska Heinsen and Corinna Bang and Roman Zenouzi and Martin Kummen and Louise Thingholm and Marie Tempel and Wolfgang Lieb and Tom Karlsen and Ansgar Lohse and Johannes Hov and Gerald Denk and Frank Lammert and Marcin Krawczyk and Christoph Schramm and Andre Franke",

note = "{\textcopyright} 2019 The Authors. Alimentary Pharmacology & Therapeutics Published by John Wiley & Sons Ltd.",

year = "2019",

month = sep,

doi = "10.1111/apt.15375",

language = "English",

volume = "50",

pages = "580--589",

journal = "ALIMENT PHARM THER",

issn = "0269-2813",

publisher = "Wiley-Blackwell",

number = "5",

}

RIS

TY - JOUR

T1 - Consistent alterations in faecal microbiomes of patients with primary sclerosing cholangitis independent of associated colitis

AU - Rühlemann, Malte

AU - Liwinski, Timur

AU - Heinsen, Femke-Anouska

AU - Bang, Corinna

AU - Zenouzi, Roman

AU - Kummen, Martin

AU - Thingholm, Louise

AU - Tempel, Marie

AU - Lieb, Wolfgang

AU - Karlsen, Tom

AU - Lohse, Ansgar

AU - Hov, Johannes

AU - Denk, Gerald

AU - Lammert, Frank

AU - Krawczyk, Marcin

AU - Schramm, Christoph

AU - Franke, Andre

PY - 2019/9

Y1 - 2019/9

N2 - BACKGROUND: Single-centre studies reported alterations of faecal microbiota in patients with primary sclerosing cholangitis (PSC). As regional factors may affect microbial communities, it is unclear if a microbial signature of PSC exists across different geographical regions.AIM: To identify a robust microbial signature of PSC independent of geography and environmental influences.METHODS: We included 388 individuals (median age, 47 years; range, 15-78) from Germany and Norway in the study, 137 patients with PSC (n = 75 with colitis), 118 with ulcerative colitis (UC) and 133 healthy controls. Faecal microbiomes were analysed by 16S rRNA gene sequencing (V1-V2). Differences in relative abundances of single taxa were subjected to a meta-analysis.RESULTS: In both cohorts, microbiota composition (beta-diversity) differed between PSC patients and controls (P < 0.001). Random forests classification discriminated PSC patients from controls in both geographical cohorts with an average area under the curve of 0.88. Compared to healthy controls, many new cohort-spanning alterations were identified in PSC, such as an increase of Proteobacteria and the bile-tolerant genus Parabacteroides, which were detected independent from geographical region. Associated colitis only had minor effects on microbiota composition, suggesting that PSC itself drives the faecal microbiota changes observed.CONCLUSION: Compared to healthy controls, numerous microbiota alterations are reproducible in PSC patients across geographical regions, clearly pointing towards a microbiota composition that is shaped by the disease itself and not by environmental factors. These reproducibly altered microbial populations might provide future insights into the pathophysiology of PSC.

AB - BACKGROUND: Single-centre studies reported alterations of faecal microbiota in patients with primary sclerosing cholangitis (PSC). As regional factors may affect microbial communities, it is unclear if a microbial signature of PSC exists across different geographical regions.AIM: To identify a robust microbial signature of PSC independent of geography and environmental influences.METHODS: We included 388 individuals (median age, 47 years; range, 15-78) from Germany and Norway in the study, 137 patients with PSC (n = 75 with colitis), 118 with ulcerative colitis (UC) and 133 healthy controls. Faecal microbiomes were analysed by 16S rRNA gene sequencing (V1-V2). Differences in relative abundances of single taxa were subjected to a meta-analysis.RESULTS: In both cohorts, microbiota composition (beta-diversity) differed between PSC patients and controls (P < 0.001). Random forests classification discriminated PSC patients from controls in both geographical cohorts with an average area under the curve of 0.88. Compared to healthy controls, many new cohort-spanning alterations were identified in PSC, such as an increase of Proteobacteria and the bile-tolerant genus Parabacteroides, which were detected independent from geographical region. Associated colitis only had minor effects on microbiota composition, suggesting that PSC itself drives the faecal microbiota changes observed.CONCLUSION: Compared to healthy controls, numerous microbiota alterations are reproducible in PSC patients across geographical regions, clearly pointing towards a microbiota composition that is shaped by the disease itself and not by environmental factors. These reproducibly altered microbial populations might provide future insights into the pathophysiology of PSC.

U2 - 10.1111/apt.15375

DO - 10.1111/apt.15375

M3 - SCORING: Journal article

C2 - 31250469

VL - 50

SP - 580

EP - 589

JO - ALIMENT PHARM THER

JF - ALIMENT PHARM THER

SN - 0269-2813

IS - 5

ER -