Comparison of contact radiographed and stained histological sections for osseointegration analysis of dental implants: an in vivo study

TY - JOUR

T1 - Comparison of contact radiographed and stained histological sections for osseointegration analysis of dental implants: an in vivo study

AU - Bissinger, Oliver

AU - Götz, Carolin

AU - Jeschke, Anke

AU - Haller, Bernhard

AU - Wolff, Klaus-Dietrich

AU - Kaiser, Pascal

AU - Kolk, Andreas

N1 - Copyright © 2017 Elsevier Inc. All rights reserved.

PY - 2018/1

Y1 - 2018/1

N2 - OBJECTIVES: Histology is still regarded as the gold-standard to determine bone implant contact (BIC) as a parameter representing implant stability. As the further processing of cut slices for contact radiography (CR) to stained and polished histological sections is time consuming and error prone, our aim was to assess agreement between CR and Giemsa-eosin (GE) stained sections with regard to dental implants.STUDY DESIGN: Threaded dental titanium implants (n = 54) from the maxillae of Goettingen minipigs were evaluated. After 28 and 56 days, BIC and the ratio of bone volume to total volume (BV/TV; 1000 μm) were determined on the same sections by using CR and GE staining, and the results were compared.RESULTS: Moderate differences for BIC (0.6%; P = .53) and BV/TV (1.3%; P = .01) between the methods were determined, in which CR overestimated BIC and BV/TV. A strong correlation was seen between the modalities concerning BIC (28 days: r = 0.84; 56 days: r = 0.85; total: r = 0.85) and BV/TV (r = 0.96; r = 0.94; r = 0.96; all: P < .0001).CONCLUSIONS: CR enabled determination of the bone-to-implant interface in comparison with GE-stained sections. BIC and BV/TV were slightly overestimated but correlated strongly between the methods. Therefore, if BIC and BV/TV are sufficient endpoints, CR is adequate and no further preparation and staining are necessary.

AB - OBJECTIVES: Histology is still regarded as the gold-standard to determine bone implant contact (BIC) as a parameter representing implant stability. As the further processing of cut slices for contact radiography (CR) to stained and polished histological sections is time consuming and error prone, our aim was to assess agreement between CR and Giemsa-eosin (GE) stained sections with regard to dental implants.STUDY DESIGN: Threaded dental titanium implants (n = 54) from the maxillae of Goettingen minipigs were evaluated. After 28 and 56 days, BIC and the ratio of bone volume to total volume (BV/TV; 1000 μm) were determined on the same sections by using CR and GE staining, and the results were compared.RESULTS: Moderate differences for BIC (0.6%; P = .53) and BV/TV (1.3%; P = .01) between the methods were determined, in which CR overestimated BIC and BV/TV. A strong correlation was seen between the modalities concerning BIC (28 days: r = 0.84; 56 days: r = 0.85; total: r = 0.85) and BV/TV (r = 0.96; r = 0.94; r = 0.96; all: P < .0001).CONCLUSIONS: CR enabled determination of the bone-to-implant interface in comparison with GE-stained sections. BIC and BV/TV were slightly overestimated but correlated strongly between the methods. Therefore, if BIC and BV/TV are sufficient endpoints, CR is adequate and no further preparation and staining are necessary.

KW - Animals

KW - Azure Stains

KW - Bone-Implant Interface

KW - Dental Implantation, Endosseous

KW - Dental Implants

KW - Maxilla

KW - Osseointegration

KW - Staining and Labeling

KW - Surface Properties

KW - Swine

KW - Swine, Miniature

KW - Titanium

KW - Comparative Study

KW - Journal Article

U2 - 10.1016/j.oooo.2017.06.118

DO - 10.1016/j.oooo.2017.06.118

M3 - SCORING: Journal article

C2 - 29079369

VL - 125

SP - 20

EP - 26

JO - OR SURG OR MED OR PA

JF - OR SURG OR MED OR PA

SN - 2212-4403

IS - 1

ER -