Comparative analysis of biofilm models to determine the efficacy of antimicrobials
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Comparative analysis of biofilm models to determine the efficacy of antimicrobials. / Stuermer, E K; Besser, M; Brill, F; Geffken, M; Plattfaut, I; Severing, A L; Wiencke, V; Rembe, J D; Naumova, E A; Kampe, A; Debus, S; Smeets, R.
In: INT J HYG ENVIR HEAL, Vol. 234, 113744, 05.2021.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Comparative analysis of biofilm models to determine the efficacy of antimicrobials
AU - Stuermer, E K
AU - Besser, M
AU - Brill, F
AU - Geffken, M
AU - Plattfaut, I
AU - Severing, A L
AU - Wiencke, V
AU - Rembe, J D
AU - Naumova, E A
AU - Kampe, A
AU - Debus, S
AU - Smeets, R
N1 - Copyright © 2021 Elsevier GmbH. All rights reserved.
PY - 2021/5
Y1 - 2021/5
N2 - Biofilms are one of the greatest challenges in today's treatment of chronic wounds. While antimicrobials kill platonic bacteria within seconds, they are rarely able to harm biofilms. In order to identify effective substances for antibacterial therapy, cost-efficient, standardized and reproducible models that aim to mimic the clinical situation are required. In this study, two 3D biofilm models based on human plasma with immune cells (lhBIOM) or based on sheep blood (sbBIOM) containing S. aureus or P. aeruginosa, are compared with the human biofilm model hpBIOM regarding their microscopic structure (scanning electron microscopy; SEM) and their bacterial resistance to octenidine hydrochloride (OCT) and a sodium hypochlorite (NaOCl) wound-irrigation solution. The three analyzed biofilm models show little to no reaction to treatment with the hypochlorous solution while planktonic S. aureus and P. aeruginosa cells are reduced within minutes. After 48 h, octenidine hydrochloride manages to erode the biofilm matrix and significantly reduce the bacterial load. The determined effects are qualitatively reflected by SEM. Our results show that both ethically acceptable human and sheep blood based biofilm models can be used as a standard for in vitro testing of new antimicrobial substances. Due to their composition, both fulfill the criteria of a reality-reflecting model and therefore should be used in the approval for new antimicrobial agents.
AB - Biofilms are one of the greatest challenges in today's treatment of chronic wounds. While antimicrobials kill platonic bacteria within seconds, they are rarely able to harm biofilms. In order to identify effective substances for antibacterial therapy, cost-efficient, standardized and reproducible models that aim to mimic the clinical situation are required. In this study, two 3D biofilm models based on human plasma with immune cells (lhBIOM) or based on sheep blood (sbBIOM) containing S. aureus or P. aeruginosa, are compared with the human biofilm model hpBIOM regarding their microscopic structure (scanning electron microscopy; SEM) and their bacterial resistance to octenidine hydrochloride (OCT) and a sodium hypochlorite (NaOCl) wound-irrigation solution. The three analyzed biofilm models show little to no reaction to treatment with the hypochlorous solution while planktonic S. aureus and P. aeruginosa cells are reduced within minutes. After 48 h, octenidine hydrochloride manages to erode the biofilm matrix and significantly reduce the bacterial load. The determined effects are qualitatively reflected by SEM. Our results show that both ethically acceptable human and sheep blood based biofilm models can be used as a standard for in vitro testing of new antimicrobial substances. Due to their composition, both fulfill the criteria of a reality-reflecting model and therefore should be used in the approval for new antimicrobial agents.
U2 - 10.1016/j.ijheh.2021.113744
DO - 10.1016/j.ijheh.2021.113744
M3 - SCORING: Journal article
C2 - 33780904
VL - 234
JO - INT J HYG ENVIR HEAL
JF - INT J HYG ENVIR HEAL
SN - 1438-4639
M1 - 113744
ER -
