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Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage

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Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage. / Matsumoto-Miyai, Kazumasa; Sokolowska, Ewa; Zurlinden, Andreas; Gee, Christine E; Lüscher, Daniel; Hettwer, Stefan; Wölfel, Jens; Ladner, Ana Paula; Ster, Jeanne; Gerber, Urs; Rülicke, Thomas; Kunz, Beat; Sonderegger, Peter.

In: CELL, Vol. 136, No. 6, 20.03.2009, p. 1161-71.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Matsumoto-Miyai, K, Sokolowska, E, Zurlinden, A, Gee, CE, Lüscher, D, Hettwer, S, Wölfel, J, Ladner, AP, Ster, J, Gerber, U, Rülicke, T, Kunz, B & Sonderegger, P 2009, 'Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage', CELL, vol. 136, no. 6, pp. 1161-71. https://doi.org/10.1016/j.cell.2009.02.034

APA

Matsumoto-Miyai, K., Sokolowska, E., Zurlinden, A., Gee, C. E., Lüscher, D., Hettwer, S., Wölfel, J., Ladner, A. P., Ster, J., Gerber, U., Rülicke, T., Kunz, B., & Sonderegger, P. (2009). Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage. CELL, 136(6), 1161-71. https://doi.org/10.1016/j.cell.2009.02.034

Vancouver

Matsumoto-Miyai K, Sokolowska E, Zurlinden A, Gee CE, Lüscher D, Hettwer S et al. Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage. CELL. 2009 Mar 20;136(6):1161-71. https://doi.org/10.1016/j.cell.2009.02.034

Bibtex

@article{c1019d5e873349e79e663a52fef613f6,
title = "Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage",
abstract = "The synaptic serine protease neurotrypsin is essential for cognitive function, as its deficiency in humans results in severe mental retardation. Recently, we demonstrated the activity-dependent release of neurotrypsin from presynaptic terminals and proteolytical cleavage of agrin at the synapse. Here we show that the activity-dependent formation of dendritic filopodia is abolished in hippocampal neurons from neurotrypsin-deficient mice. Administration of the neurotrypsin-dependent 22 kDa fragment of agrin rescues the filopodial response. Detailed analyses indicated that presynaptic action potential firing is necessary for the release of neurotrypsin, whereas postsynaptic NMDA receptor activation is necessary for the neurotrypsin-dependent cleavage of agrin. This contingency characterizes the neurotrypsin-agrin system as a coincidence detector of pre- and postsynaptic activation. As the resulting dendritic filopodia are thought to represent precursors of synapses, the neurotrypsin-dependent cleavage of agrin at the synapse may be instrumental for a Hebbian organization and remodeling of synaptic circuits in the CNS.",
keywords = "Agrin, Animals, Cell Line, Dendrites, Exocytosis, Hippocampus, Humans, In Vitro Techniques, Mice, Mice, Transgenic, Mutagenesis, Presynaptic Terminals, Pseudopodia, Serine Endopeptidases",
author = "Kazumasa Matsumoto-Miyai and Ewa Sokolowska and Andreas Zurlinden and Gee, {Christine E} and Daniel L{\"u}scher and Stefan Hettwer and Jens W{\"o}lfel and Ladner, {Ana Paula} and Jeanne Ster and Urs Gerber and Thomas R{\"u}licke and Beat Kunz and Peter Sonderegger",
year = "2009",
month = mar,
day = "20",
doi = "10.1016/j.cell.2009.02.034",
language = "English",
volume = "136",
pages = "1161--71",
journal = "CELL",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - Coincident pre- and postsynaptic activation induces dendritic filopodia via neurotrypsin-dependent agrin cleavage

AU - Matsumoto-Miyai, Kazumasa

AU - Sokolowska, Ewa

AU - Zurlinden, Andreas

AU - Gee, Christine E

AU - Lüscher, Daniel

AU - Hettwer, Stefan

AU - Wölfel, Jens

AU - Ladner, Ana Paula

AU - Ster, Jeanne

AU - Gerber, Urs

AU - Rülicke, Thomas

AU - Kunz, Beat

AU - Sonderegger, Peter

PY - 2009/3/20

Y1 - 2009/3/20

N2 - The synaptic serine protease neurotrypsin is essential for cognitive function, as its deficiency in humans results in severe mental retardation. Recently, we demonstrated the activity-dependent release of neurotrypsin from presynaptic terminals and proteolytical cleavage of agrin at the synapse. Here we show that the activity-dependent formation of dendritic filopodia is abolished in hippocampal neurons from neurotrypsin-deficient mice. Administration of the neurotrypsin-dependent 22 kDa fragment of agrin rescues the filopodial response. Detailed analyses indicated that presynaptic action potential firing is necessary for the release of neurotrypsin, whereas postsynaptic NMDA receptor activation is necessary for the neurotrypsin-dependent cleavage of agrin. This contingency characterizes the neurotrypsin-agrin system as a coincidence detector of pre- and postsynaptic activation. As the resulting dendritic filopodia are thought to represent precursors of synapses, the neurotrypsin-dependent cleavage of agrin at the synapse may be instrumental for a Hebbian organization and remodeling of synaptic circuits in the CNS.

AB - The synaptic serine protease neurotrypsin is essential for cognitive function, as its deficiency in humans results in severe mental retardation. Recently, we demonstrated the activity-dependent release of neurotrypsin from presynaptic terminals and proteolytical cleavage of agrin at the synapse. Here we show that the activity-dependent formation of dendritic filopodia is abolished in hippocampal neurons from neurotrypsin-deficient mice. Administration of the neurotrypsin-dependent 22 kDa fragment of agrin rescues the filopodial response. Detailed analyses indicated that presynaptic action potential firing is necessary for the release of neurotrypsin, whereas postsynaptic NMDA receptor activation is necessary for the neurotrypsin-dependent cleavage of agrin. This contingency characterizes the neurotrypsin-agrin system as a coincidence detector of pre- and postsynaptic activation. As the resulting dendritic filopodia are thought to represent precursors of synapses, the neurotrypsin-dependent cleavage of agrin at the synapse may be instrumental for a Hebbian organization and remodeling of synaptic circuits in the CNS.

KW - Agrin

KW - Animals

KW - Cell Line

KW - Dendrites

KW - Exocytosis

KW - Hippocampus

KW - Humans

KW - In Vitro Techniques

KW - Mice

KW - Mice, Transgenic

KW - Mutagenesis

KW - Presynaptic Terminals

KW - Pseudopodia

KW - Serine Endopeptidases

U2 - 10.1016/j.cell.2009.02.034

DO - 10.1016/j.cell.2009.02.034

M3 - SCORING: Journal article

C2 - 19303856

VL - 136

SP - 1161

EP - 1171

JO - CELL

JF - CELL

SN - 0092-8674

IS - 6

ER -