Cloning and sequencing of Enterobacter aerogenes OmpC-type osmoporin linked to carbapenem resistance.
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Cloning and sequencing of Enterobacter aerogenes OmpC-type osmoporin linked to carbapenem resistance. / Stürenburg, Enno; Sobottka, Ingo; Mack, Dietrich; Laufs, Rainer.
In: INT J MED MICROBIOL, Vol. 291, No. 8, 8, 2002, p. 649-654.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Cloning and sequencing of Enterobacter aerogenes OmpC-type osmoporin linked to carbapenem resistance.
AU - Stürenburg, Enno
AU - Sobottka, Ingo
AU - Mack, Dietrich
AU - Laufs, Rainer
PY - 2002
Y1 - 2002
N2 - Using outbreak-related strains of Enterobacter aerogenes, we cloned and sequenced ompK39, the structural gene coding for outer membrane protein OmpK39. Its lack of expression was closely associated with a phenotype exhibiting low-level carbapenem resistance. Detailed alignment of the predicted amino acid sequence revealed that OmpK39 is a member of the OmpC subclass of enterobacterial porins, with the highest degree of homology to Klebsiella pneumoniae OmpK36. Based on a computerized alignment including Escherichia coli PhoE and OmpF, the 3D structures of which are known from X-ray studies, OmpK39 can be assumed to form the typical beta-barrel structure which is common to all enterobacterial porins. Since no inhibitory DNA sequences could be detected in ompk39 in the resistant strains, porin deficiency leading to carbapenem resistance seems to involve alterations in key regulatory genes and/or the promotor sequence rather than a direct mutation in the structural gene.
AB - Using outbreak-related strains of Enterobacter aerogenes, we cloned and sequenced ompK39, the structural gene coding for outer membrane protein OmpK39. Its lack of expression was closely associated with a phenotype exhibiting low-level carbapenem resistance. Detailed alignment of the predicted amino acid sequence revealed that OmpK39 is a member of the OmpC subclass of enterobacterial porins, with the highest degree of homology to Klebsiella pneumoniae OmpK36. Based on a computerized alignment including Escherichia coli PhoE and OmpF, the 3D structures of which are known from X-ray studies, OmpK39 can be assumed to form the typical beta-barrel structure which is common to all enterobacterial porins. Since no inhibitory DNA sequences could be detected in ompk39 in the resistant strains, porin deficiency leading to carbapenem resistance seems to involve alterations in key regulatory genes and/or the promotor sequence rather than a direct mutation in the structural gene.
M3 - SCORING: Zeitschriftenaufsatz
VL - 291
SP - 649
EP - 654
JO - INT J MED MICROBIOL
JF - INT J MED MICROBIOL
SN - 1438-4221
IS - 8
M1 - 8
ER -
