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Cloning and functional expression of rat ether-à-go-go-like K+ channel genes

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Cloning and functional expression of rat ether-à-go-go-like K+ channel genes. / Engeland, B; Neu, A; Ludwig, J; Roeper, J; Pongs, O.

In: J PHYSIOL-LONDON, Vol. 513 ( Pt 3), 15.12.1998, p. 647-54.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Engeland, B, Neu, A, Ludwig, J, Roeper, J & Pongs, O 1998, 'Cloning and functional expression of rat ether-à-go-go-like K+ channel genes', J PHYSIOL-LONDON, vol. 513 ( Pt 3), pp. 647-54.

APA

Engeland, B., Neu, A., Ludwig, J., Roeper, J., & Pongs, O. (1998). Cloning and functional expression of rat ether-à-go-go-like K+ channel genes. J PHYSIOL-LONDON, 513 ( Pt 3), 647-54.

Vancouver

Engeland B, Neu A, Ludwig J, Roeper J, Pongs O. Cloning and functional expression of rat ether-à-go-go-like K+ channel genes. J PHYSIOL-LONDON. 1998 Dec 15;513 ( Pt 3):647-54.

Bibtex

@article{f34df9ad95ae4373a80b7d6a101f4e35,
title = "Cloning and functional expression of rat ether-{\`a}-go-go-like K+ channel genes",
abstract = "1. Screening of rat cortex cDNA resulted in cloning of two complete and one partial orthologue of the Drosophila ether-{\`a}-go-go-like K+ channel (elk). 2. Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed predominant expression of rat elk mRNAs in brain. Each rat elk mRNA showed a distinct, but overlapping expression pattern in different rat brain areas. 3. Transient transfection of Chinese hamster ovary (CHO) cells with rat elk1 or rat elk2 cDNA gave rise to voltage-activated K+ channels with novel properties. 4. RELK1 channels mediated slowly activating sustained potassium currents. The threshold for activation was at -90 mV. Currents were insensitive to tetraethylammonium (TEA) and 4-aminopyridine (4-AP), but were blocked by micromolar concentrations of Ba2+. RELK1 activation kinetics were not dependent on prepulse potential like REAG-mediated currents. 5. RELK2 channels produced currents with a fast inactivation component and HERG-like tail currents. RELK2 currents were not sensitive to the HERG channel blocker E4031.",
keywords = "Amino Acid Sequence, Animals, Blotting, Northern, CHO Cells, Cricetinae, DNA, Complementary, Electric Stimulation, Electrophysiology, Membrane Potentials, Molecular Sequence Data, Oncogene Proteins, Patch-Clamp Techniques, Potassium Channels, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors",
author = "B Engeland and A Neu and J Ludwig and J Roeper and O Pongs",
year = "1998",
month = dec,
day = "15",
language = "English",
volume = "513 ( Pt 3)",
pages = "647--54",
journal = "J PHYSIOL-LONDON",
issn = "0022-3751",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Cloning and functional expression of rat ether-à-go-go-like K+ channel genes

AU - Engeland, B

AU - Neu, A

AU - Ludwig, J

AU - Roeper, J

AU - Pongs, O

PY - 1998/12/15

Y1 - 1998/12/15

N2 - 1. Screening of rat cortex cDNA resulted in cloning of two complete and one partial orthologue of the Drosophila ether-à-go-go-like K+ channel (elk). 2. Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed predominant expression of rat elk mRNAs in brain. Each rat elk mRNA showed a distinct, but overlapping expression pattern in different rat brain areas. 3. Transient transfection of Chinese hamster ovary (CHO) cells with rat elk1 or rat elk2 cDNA gave rise to voltage-activated K+ channels with novel properties. 4. RELK1 channels mediated slowly activating sustained potassium currents. The threshold for activation was at -90 mV. Currents were insensitive to tetraethylammonium (TEA) and 4-aminopyridine (4-AP), but were blocked by micromolar concentrations of Ba2+. RELK1 activation kinetics were not dependent on prepulse potential like REAG-mediated currents. 5. RELK2 channels produced currents with a fast inactivation component and HERG-like tail currents. RELK2 currents were not sensitive to the HERG channel blocker E4031.

AB - 1. Screening of rat cortex cDNA resulted in cloning of two complete and one partial orthologue of the Drosophila ether-à-go-go-like K+ channel (elk). 2. Northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed predominant expression of rat elk mRNAs in brain. Each rat elk mRNA showed a distinct, but overlapping expression pattern in different rat brain areas. 3. Transient transfection of Chinese hamster ovary (CHO) cells with rat elk1 or rat elk2 cDNA gave rise to voltage-activated K+ channels with novel properties. 4. RELK1 channels mediated slowly activating sustained potassium currents. The threshold for activation was at -90 mV. Currents were insensitive to tetraethylammonium (TEA) and 4-aminopyridine (4-AP), but were blocked by micromolar concentrations of Ba2+. RELK1 activation kinetics were not dependent on prepulse potential like REAG-mediated currents. 5. RELK2 channels produced currents with a fast inactivation component and HERG-like tail currents. RELK2 currents were not sensitive to the HERG channel blocker E4031.

KW - Amino Acid Sequence

KW - Animals

KW - Blotting, Northern

KW - CHO Cells

KW - Cricetinae

KW - DNA, Complementary

KW - Electric Stimulation

KW - Electrophysiology

KW - Membrane Potentials

KW - Molecular Sequence Data

KW - Oncogene Proteins

KW - Patch-Clamp Techniques

KW - Potassium Channels

KW - Rats

KW - Rats, Sprague-Dawley

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Transcription Factors

M3 - SCORING: Journal article

C2 - 9824707

VL - 513 ( Pt 3)

SP - 647

EP - 654

JO - J PHYSIOL-LONDON

JF - J PHYSIOL-LONDON

SN - 0022-3751

ER -