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CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry

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CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry. / Heinl, Elena-Sofia; Lorenz, Sebastian; Schmidt, Barbara; Nasser M Laqtom, Nouf; Mazzulli, Joseph R; Francelle, Laetitia; Yu, Timothy W; Greenberg, Benjamin; Storch, Stephan; Tegtmeier, Ines; Othmen, Helga; Maurer, Katja; Steinfurth, Malin; Witzgall, Ralph; Milenkovic, Vladimir; Wetzel, Christian H; Reichold, Markus.

In: ISCIENCE, Vol. 25, No. 10, 105082, 21.10.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Heinl, E-S, Lorenz, S, Schmidt, B, Nasser M Laqtom, N, Mazzulli, JR, Francelle, L, Yu, TW, Greenberg, B, Storch, S, Tegtmeier, I, Othmen, H, Maurer, K, Steinfurth, M, Witzgall, R, Milenkovic, V, Wetzel, CH & Reichold, M 2022, 'CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry', ISCIENCE, vol. 25, no. 10, 105082. https://doi.org/10.1016/j.isci.2022.105082

APA

Heinl, E-S., Lorenz, S., Schmidt, B., Nasser M Laqtom, N., Mazzulli, J. R., Francelle, L., Yu, T. W., Greenberg, B., Storch, S., Tegtmeier, I., Othmen, H., Maurer, K., Steinfurth, M., Witzgall, R., Milenkovic, V., Wetzel, C. H., & Reichold, M. (2022). CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry. ISCIENCE, 25(10), [105082]. https://doi.org/10.1016/j.isci.2022.105082

Vancouver

Heinl E-S, Lorenz S, Schmidt B, Nasser M Laqtom N, Mazzulli JR, Francelle L et al. CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry. ISCIENCE. 2022 Oct 21;25(10). 105082. https://doi.org/10.1016/j.isci.2022.105082

Bibtex

@article{906faf13566244a2adc482c74c3a48ba,
title = "CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry",
abstract = "The SARS-CoV-2 virus has triggered a worldwide pandemic. According to the BioGrid database, CLN7 (MFSD8) is thought to interact with several viral proteins. The aim of this work was to investigate a possible involvement of CLN7 in the infection process. Experiments on a CLN7-deficient HEK293T cell line exhibited a 90% reduced viral load compared to wild-type cells. This observation may be linked to the finding that CLN7 ko cells have a significantly reduced GM1 content in their cell membrane. GM1 is found highly enriched in lipid rafts, which are thought to play an important role in SARS-CoV-2 infection. In contrast, overexpression of CLN7 led to an increase in viral load. This study provides evidence that CLN7 is involved in SARS-CoV-2 infection. This makes it a potential pharmacological target for drug development against COVID-19. Furthermore, it provides insights into the physiological function of CLN7 where still only little is known about.",
author = "Elena-Sofia Heinl and Sebastian Lorenz and Barbara Schmidt and {Nasser M Laqtom}, Nouf and Mazzulli, {Joseph R} and Laetitia Francelle and Yu, {Timothy W} and Benjamin Greenberg and Stephan Storch and Ines Tegtmeier and Helga Othmen and Katja Maurer and Malin Steinfurth and Ralph Witzgall and Vladimir Milenkovic and Wetzel, {Christian H} and Markus Reichold",
note = "{\textcopyright} 2022.",
year = "2022",
month = oct,
day = "21",
doi = "10.1016/j.isci.2022.105082",
language = "English",
volume = "25",
journal = "ISCIENCE",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "10",

}

RIS

TY - JOUR

T1 - CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry

AU - Heinl, Elena-Sofia

AU - Lorenz, Sebastian

AU - Schmidt, Barbara

AU - Nasser M Laqtom, Nouf

AU - Mazzulli, Joseph R

AU - Francelle, Laetitia

AU - Yu, Timothy W

AU - Greenberg, Benjamin

AU - Storch, Stephan

AU - Tegtmeier, Ines

AU - Othmen, Helga

AU - Maurer, Katja

AU - Steinfurth, Malin

AU - Witzgall, Ralph

AU - Milenkovic, Vladimir

AU - Wetzel, Christian H

AU - Reichold, Markus

N1 - © 2022.

PY - 2022/10/21

Y1 - 2022/10/21

N2 - The SARS-CoV-2 virus has triggered a worldwide pandemic. According to the BioGrid database, CLN7 (MFSD8) is thought to interact with several viral proteins. The aim of this work was to investigate a possible involvement of CLN7 in the infection process. Experiments on a CLN7-deficient HEK293T cell line exhibited a 90% reduced viral load compared to wild-type cells. This observation may be linked to the finding that CLN7 ko cells have a significantly reduced GM1 content in their cell membrane. GM1 is found highly enriched in lipid rafts, which are thought to play an important role in SARS-CoV-2 infection. In contrast, overexpression of CLN7 led to an increase in viral load. This study provides evidence that CLN7 is involved in SARS-CoV-2 infection. This makes it a potential pharmacological target for drug development against COVID-19. Furthermore, it provides insights into the physiological function of CLN7 where still only little is known about.

AB - The SARS-CoV-2 virus has triggered a worldwide pandemic. According to the BioGrid database, CLN7 (MFSD8) is thought to interact with several viral proteins. The aim of this work was to investigate a possible involvement of CLN7 in the infection process. Experiments on a CLN7-deficient HEK293T cell line exhibited a 90% reduced viral load compared to wild-type cells. This observation may be linked to the finding that CLN7 ko cells have a significantly reduced GM1 content in their cell membrane. GM1 is found highly enriched in lipid rafts, which are thought to play an important role in SARS-CoV-2 infection. In contrast, overexpression of CLN7 led to an increase in viral load. This study provides evidence that CLN7 is involved in SARS-CoV-2 infection. This makes it a potential pharmacological target for drug development against COVID-19. Furthermore, it provides insights into the physiological function of CLN7 where still only little is known about.

U2 - 10.1016/j.isci.2022.105082

DO - 10.1016/j.isci.2022.105082

M3 - SCORING: Journal article

C2 - 36093380

VL - 25

JO - ISCIENCE

JF - ISCIENCE

SN - 2589-0042

IS - 10

M1 - 105082

ER -