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Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus

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Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus. / Nörz, Dominik; Brehm, Thomas Theo; Tang, Hui Ting; Grewe, Ilka; Hermanussen, Lennart; Matthews, Hanna; Pestel, Julia; Degen, Olaf; Günther, Thomas; Grundhoff, Adam; Fischer, Nicole; Addo, Marylyn M; Jordan, Sabine; Hertling, Sandra; Unger, Stephan; Schäfer, Guido; Schewe, Knud; Hoffmann, Christian; Aepfelbacher, Martin; Pfefferle, Susanne; Schulze Zur Wiesch, Julian; Schmiedel, Stefan; Lütgehetmann, Marc.

In: J CLIN VIROL, Vol. 155, 105254, 10.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Nörz, D, Brehm, TT, Tang, HT, Grewe, I, Hermanussen, L, Matthews, H, Pestel, J, Degen, O, Günther, T, Grundhoff, A, Fischer, N, Addo, MM, Jordan, S, Hertling, S, Unger, S, Schäfer, G, Schewe, K, Hoffmann, C, Aepfelbacher, M, Pfefferle, S, Schulze Zur Wiesch, J, Schmiedel, S & Lütgehetmann, M 2022, 'Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus', J CLIN VIROL, vol. 155, 105254. https://doi.org/10.1016/j.jcv.2022.105254

APA

Nörz, D., Brehm, T. T., Tang, H. T., Grewe, I., Hermanussen, L., Matthews, H., Pestel, J., Degen, O., Günther, T., Grundhoff, A., Fischer, N., Addo, M. M., Jordan, S., Hertling, S., Unger, S., Schäfer, G., Schewe, K., Hoffmann, C., Aepfelbacher, M., ... Lütgehetmann, M. (2022). Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus. J CLIN VIROL, 155, [105254]. https://doi.org/10.1016/j.jcv.2022.105254

Vancouver

Nörz D, Brehm TT, Tang HT, Grewe I, Hermanussen L, Matthews H et al. Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus. J CLIN VIROL. 2022 Oct;155. 105254. https://doi.org/10.1016/j.jcv.2022.105254

Bibtex

@article{86133a6dea9644e3bca7e81321018067,
title = "Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus",
abstract = "BACKGROUND: The ongoing monkeypox virus outbreak includes at least 7553 confirmed cases in previously non-endemic countries worldwide as of July 2022. Clinical presentation has been reported as highly variable, sometimes lacking classically described systemic symptoms, and only small numbers of cutaneous lesions in most patients. The aim of this study was to compare clinical data with longitudinal qPCR results from lesion swabs, oropharyngeal swabs and blood in a well characterized patient cohort.METHODS: 16 male patients (5 hospitalized, 11 outpatients) were included in the study cohort and serial testing for monkeypox virus-DNA carried out in various materials throughout the course of disease. Laboratory analysis included quantitative PCR, next-generation sequencing, immunofluorescence tests and virus isolation in cell culture.RESULTS: All patients were male, between age 20 and 60, and self-identified as men having sex with men. Two had a known HIV infection, coinciding with an increased number of lesions and viral DNA detectable in blood. In initial- and serial testing, lesion swabs yielded viral DNA-loads at, or above 106 cp/ml and only declined during the third week. Oropharyngeal swabs featured lower viral loads and returned repeatedly negative in some cases. Viral culture was successful only from lesion swabs but not from oropharyngeal swabs or plasma.DISCUSSION: The data presented underscore the reliability of lesion swabs for monkeypox virus-detection, even in later stages of the disease. Oropharyngeal swabs and blood samples alone carry the risk of false negative results, but may hold value in pre-/asymptomatic cases or viral load monitoring, respectively.",
keywords = "Adult, DNA, Viral, Female, HIV Infections, Humans, Male, Middle Aged, Monkeypox/diagnosis, Monkeypox virus/genetics, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Young Adult",
author = "Dominik N{\"o}rz and Brehm, {Thomas Theo} and Tang, {Hui Ting} and Ilka Grewe and Lennart Hermanussen and Hanna Matthews and Julia Pestel and Olaf Degen and Thomas G{\"u}nther and Adam Grundhoff and Nicole Fischer and Addo, {Marylyn M} and Sabine Jordan and Sandra Hertling and Stephan Unger and Guido Sch{\"a}fer and Knud Schewe and Christian Hoffmann and Martin Aepfelbacher and Susanne Pfefferle and {Schulze Zur Wiesch}, Julian and Stefan Schmiedel and Marc L{\"u}tgehetmann",
note = "Copyright {\textcopyright} 2022. Published by Elsevier B.V.",
year = "2022",
month = oct,
doi = "10.1016/j.jcv.2022.105254",
language = "English",
volume = "155",
journal = "J CLIN VIROL",
issn = "1386-6532",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Clinical characteristics and comparison of longitudinal qPCR results from different specimen types in a cohort of ambulatory and hospitalized patients infected with monkeypox virus

AU - Nörz, Dominik

AU - Brehm, Thomas Theo

AU - Tang, Hui Ting

AU - Grewe, Ilka

AU - Hermanussen, Lennart

AU - Matthews, Hanna

AU - Pestel, Julia

AU - Degen, Olaf

AU - Günther, Thomas

AU - Grundhoff, Adam

AU - Fischer, Nicole

AU - Addo, Marylyn M

AU - Jordan, Sabine

AU - Hertling, Sandra

AU - Unger, Stephan

AU - Schäfer, Guido

AU - Schewe, Knud

AU - Hoffmann, Christian

AU - Aepfelbacher, Martin

AU - Pfefferle, Susanne

AU - Schulze Zur Wiesch, Julian

AU - Schmiedel, Stefan

AU - Lütgehetmann, Marc

N1 - Copyright © 2022. Published by Elsevier B.V.

PY - 2022/10

Y1 - 2022/10

N2 - BACKGROUND: The ongoing monkeypox virus outbreak includes at least 7553 confirmed cases in previously non-endemic countries worldwide as of July 2022. Clinical presentation has been reported as highly variable, sometimes lacking classically described systemic symptoms, and only small numbers of cutaneous lesions in most patients. The aim of this study was to compare clinical data with longitudinal qPCR results from lesion swabs, oropharyngeal swabs and blood in a well characterized patient cohort.METHODS: 16 male patients (5 hospitalized, 11 outpatients) were included in the study cohort and serial testing for monkeypox virus-DNA carried out in various materials throughout the course of disease. Laboratory analysis included quantitative PCR, next-generation sequencing, immunofluorescence tests and virus isolation in cell culture.RESULTS: All patients were male, between age 20 and 60, and self-identified as men having sex with men. Two had a known HIV infection, coinciding with an increased number of lesions and viral DNA detectable in blood. In initial- and serial testing, lesion swabs yielded viral DNA-loads at, or above 106 cp/ml and only declined during the third week. Oropharyngeal swabs featured lower viral loads and returned repeatedly negative in some cases. Viral culture was successful only from lesion swabs but not from oropharyngeal swabs or plasma.DISCUSSION: The data presented underscore the reliability of lesion swabs for monkeypox virus-detection, even in later stages of the disease. Oropharyngeal swabs and blood samples alone carry the risk of false negative results, but may hold value in pre-/asymptomatic cases or viral load monitoring, respectively.

AB - BACKGROUND: The ongoing monkeypox virus outbreak includes at least 7553 confirmed cases in previously non-endemic countries worldwide as of July 2022. Clinical presentation has been reported as highly variable, sometimes lacking classically described systemic symptoms, and only small numbers of cutaneous lesions in most patients. The aim of this study was to compare clinical data with longitudinal qPCR results from lesion swabs, oropharyngeal swabs and blood in a well characterized patient cohort.METHODS: 16 male patients (5 hospitalized, 11 outpatients) were included in the study cohort and serial testing for monkeypox virus-DNA carried out in various materials throughout the course of disease. Laboratory analysis included quantitative PCR, next-generation sequencing, immunofluorescence tests and virus isolation in cell culture.RESULTS: All patients were male, between age 20 and 60, and self-identified as men having sex with men. Two had a known HIV infection, coinciding with an increased number of lesions and viral DNA detectable in blood. In initial- and serial testing, lesion swabs yielded viral DNA-loads at, or above 106 cp/ml and only declined during the third week. Oropharyngeal swabs featured lower viral loads and returned repeatedly negative in some cases. Viral culture was successful only from lesion swabs but not from oropharyngeal swabs or plasma.DISCUSSION: The data presented underscore the reliability of lesion swabs for monkeypox virus-detection, even in later stages of the disease. Oropharyngeal swabs and blood samples alone carry the risk of false negative results, but may hold value in pre-/asymptomatic cases or viral load monitoring, respectively.

KW - Adult

KW - DNA, Viral

KW - Female

KW - HIV Infections

KW - Humans

KW - Male

KW - Middle Aged

KW - Monkeypox/diagnosis

KW - Monkeypox virus/genetics

KW - Real-Time Polymerase Chain Reaction

KW - Reproducibility of Results

KW - Young Adult

U2 - 10.1016/j.jcv.2022.105254

DO - 10.1016/j.jcv.2022.105254

M3 - SCORING: Journal article

C2 - 36057206

VL - 155

JO - J CLIN VIROL

JF - J CLIN VIROL

SN - 1386-6532

M1 - 105254

ER -