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Circulating endothelial cells as potential diagnostic biomarkers in primary central nervous system vasculitis

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Circulating endothelial cells as potential diagnostic biomarkers in primary central nervous system vasculitis. / Deb-Chatterji, Milani; Gerdes, Stefan; Heeren, Meike; Lambrecht, Jürgen; Worthmann, Hans; Goldbecker, Annemarie; Tryc, Anita Blanka; Lovric, Svjetlana; Schulz-Schaeffer, Walter; Brandis, Almuth; Dengler, Reinhard; Weissenborn, Karin; Haubitz, Marion.

In: J NEUROL NEUROSUR PS, Vol. 84, No. 7, 07.2013, p. 732-4.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Deb-Chatterji, M, Gerdes, S, Heeren, M, Lambrecht, J, Worthmann, H, Goldbecker, A, Tryc, AB, Lovric, S, Schulz-Schaeffer, W, Brandis, A, Dengler, R, Weissenborn, K & Haubitz, M 2013, 'Circulating endothelial cells as potential diagnostic biomarkers in primary central nervous system vasculitis', J NEUROL NEUROSUR PS, vol. 84, no. 7, pp. 732-4. https://doi.org/10.1136/jnnp-2012-303335

APA

Deb-Chatterji, M., Gerdes, S., Heeren, M., Lambrecht, J., Worthmann, H., Goldbecker, A., Tryc, A. B., Lovric, S., Schulz-Schaeffer, W., Brandis, A., Dengler, R., Weissenborn, K., & Haubitz, M. (2013). Circulating endothelial cells as potential diagnostic biomarkers in primary central nervous system vasculitis. J NEUROL NEUROSUR PS, 84(7), 732-4. https://doi.org/10.1136/jnnp-2012-303335

Vancouver

Deb-Chatterji M, Gerdes S, Heeren M, Lambrecht J, Worthmann H, Goldbecker A et al. Circulating endothelial cells as potential diagnostic biomarkers in primary central nervous system vasculitis. J NEUROL NEUROSUR PS. 2013 Jul;84(7):732-4. https://doi.org/10.1136/jnnp-2012-303335

Bibtex

@article{8e1c154bb2594526bf037f6c2f203a31,
title = "Circulating endothelial cells as potential diagnostic biomarkers in primary central nervous system vasculitis",
abstract = "OBJECTIVE: Histological evidence is considered the only proof of primary central nervous system vasculitis (PCNSV). However, brain biopsy is often omitted or delayed because of the invasiveness and possible complications of the procedure. Circulating endothelial cells (CEC) were shown to be elevated in patients with active antineutrophil cytoplasmic antibody-associated vasculitis. We hypothesise that CEC are also elevated in patients with active PCNSV and may contribute to the diagnosis.METHODS: CEC were assessed in 18 patients, 3 of whom had biopsy-proven PCNSV and 15 clinical, cerebrospinal fluid and imaging data, highly suggestive of PCNSV. In 3 of these 15 patients CEC assessment was performed after initiation of successful immunosuppressive therapy. CEC numbers of all patients were compared to those of 16 healthy volunteers and 123 subjects with cerebrovascular risk factors and/or ischaemic stroke, who had been studied in our group before. CEC were assessed by immunomagnetic isolation from peripheral blood.RESULTS: In patients with proven and suspected active PCNSV, CEC were extremely elevated (>400 cells/ml in most of the patients) and significantly higher than in healthy and disease controls (p≤0.01 for each group). CEC significantly decreased with immunosuppressive treatment.CONCLUSIONS: For the first time it is shown that CEC are significantly elevated in patients with active PCNSV in contrast to other pathologies associated with brain infarction and correlate with disease activity. Sensitivity and specificity of the method for diagnosing PCNSV and the use of the method for treatment monitoring should be addressed in future prospective studies with a larger patient group.",
keywords = "Adult, Aged, Aged, 80 and over, Biomarkers, Biopsy, Brain, Brain Ischemia, Cerebral Infarction, Endothelial Cells, Female, Humans, Immunosuppressive Agents, Male, Middle Aged, Monitoring, Physiologic, Prospective Studies, Risk Factors, Stroke, Treatment Outcome, Vasculitis, Central Nervous System, Journal Article",
author = "Milani Deb-Chatterji and Stefan Gerdes and Meike Heeren and J{\"u}rgen Lambrecht and Hans Worthmann and Annemarie Goldbecker and Tryc, {Anita Blanka} and Svjetlana Lovric and Walter Schulz-Schaeffer and Almuth Brandis and Reinhard Dengler and Karin Weissenborn and Marion Haubitz",
year = "2013",
month = jul,
doi = "10.1136/jnnp-2012-303335",
language = "English",
volume = "84",
pages = "732--4",
journal = "J NEUROL NEUROSUR PS",
issn = "0022-3050",
publisher = "BMJ PUBLISHING GROUP",
number = "7",

}

RIS

TY - JOUR

T1 - Circulating endothelial cells as potential diagnostic biomarkers in primary central nervous system vasculitis

AU - Deb-Chatterji, Milani

AU - Gerdes, Stefan

AU - Heeren, Meike

AU - Lambrecht, Jürgen

AU - Worthmann, Hans

AU - Goldbecker, Annemarie

AU - Tryc, Anita Blanka

AU - Lovric, Svjetlana

AU - Schulz-Schaeffer, Walter

AU - Brandis, Almuth

AU - Dengler, Reinhard

AU - Weissenborn, Karin

AU - Haubitz, Marion

PY - 2013/7

Y1 - 2013/7

N2 - OBJECTIVE: Histological evidence is considered the only proof of primary central nervous system vasculitis (PCNSV). However, brain biopsy is often omitted or delayed because of the invasiveness and possible complications of the procedure. Circulating endothelial cells (CEC) were shown to be elevated in patients with active antineutrophil cytoplasmic antibody-associated vasculitis. We hypothesise that CEC are also elevated in patients with active PCNSV and may contribute to the diagnosis.METHODS: CEC were assessed in 18 patients, 3 of whom had biopsy-proven PCNSV and 15 clinical, cerebrospinal fluid and imaging data, highly suggestive of PCNSV. In 3 of these 15 patients CEC assessment was performed after initiation of successful immunosuppressive therapy. CEC numbers of all patients were compared to those of 16 healthy volunteers and 123 subjects with cerebrovascular risk factors and/or ischaemic stroke, who had been studied in our group before. CEC were assessed by immunomagnetic isolation from peripheral blood.RESULTS: In patients with proven and suspected active PCNSV, CEC were extremely elevated (>400 cells/ml in most of the patients) and significantly higher than in healthy and disease controls (p≤0.01 for each group). CEC significantly decreased with immunosuppressive treatment.CONCLUSIONS: For the first time it is shown that CEC are significantly elevated in patients with active PCNSV in contrast to other pathologies associated with brain infarction and correlate with disease activity. Sensitivity and specificity of the method for diagnosing PCNSV and the use of the method for treatment monitoring should be addressed in future prospective studies with a larger patient group.

AB - OBJECTIVE: Histological evidence is considered the only proof of primary central nervous system vasculitis (PCNSV). However, brain biopsy is often omitted or delayed because of the invasiveness and possible complications of the procedure. Circulating endothelial cells (CEC) were shown to be elevated in patients with active antineutrophil cytoplasmic antibody-associated vasculitis. We hypothesise that CEC are also elevated in patients with active PCNSV and may contribute to the diagnosis.METHODS: CEC were assessed in 18 patients, 3 of whom had biopsy-proven PCNSV and 15 clinical, cerebrospinal fluid and imaging data, highly suggestive of PCNSV. In 3 of these 15 patients CEC assessment was performed after initiation of successful immunosuppressive therapy. CEC numbers of all patients were compared to those of 16 healthy volunteers and 123 subjects with cerebrovascular risk factors and/or ischaemic stroke, who had been studied in our group before. CEC were assessed by immunomagnetic isolation from peripheral blood.RESULTS: In patients with proven and suspected active PCNSV, CEC were extremely elevated (>400 cells/ml in most of the patients) and significantly higher than in healthy and disease controls (p≤0.01 for each group). CEC significantly decreased with immunosuppressive treatment.CONCLUSIONS: For the first time it is shown that CEC are significantly elevated in patients with active PCNSV in contrast to other pathologies associated with brain infarction and correlate with disease activity. Sensitivity and specificity of the method for diagnosing PCNSV and the use of the method for treatment monitoring should be addressed in future prospective studies with a larger patient group.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Biomarkers

KW - Biopsy

KW - Brain

KW - Brain Ischemia

KW - Cerebral Infarction

KW - Endothelial Cells

KW - Female

KW - Humans

KW - Immunosuppressive Agents

KW - Male

KW - Middle Aged

KW - Monitoring, Physiologic

KW - Prospective Studies

KW - Risk Factors

KW - Stroke

KW - Treatment Outcome

KW - Vasculitis, Central Nervous System

KW - Journal Article

U2 - 10.1136/jnnp-2012-303335

DO - 10.1136/jnnp-2012-303335

M3 - SCORING: Journal article

C2 - 23243263

VL - 84

SP - 732

EP - 734

JO - J NEUROL NEUROSUR PS

JF - J NEUROL NEUROSUR PS

SN - 0022-3050

IS - 7

ER -