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Cholesterol Regulates Syntaxin 6 Trafficking at trans-Golgi Network Endosomal Boundaries

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Cholesterol Regulates Syntaxin 6 Trafficking at trans-Golgi Network Endosomal Boundaries. / Reverter, Meritxell; Rentero, Carles; Garcia-Melero, Ana; Hoque, Monira; Vilà de Muga, Sandra; Alvarez-Guaita, Anna; Conway, James R W; Wood, Peta; Cairns, Rose; Lykopoulou, Lilia; Grinberg, Daniel; Vilageliu, Lluïsa; Bosch, Marta; Heeren, Joerg; Blasi, Juan; Timpson, Paul; Pol, Albert; Tebar, Francesc; Murray, Rachael Z; Grewal, Thomas; Enrich, Carlos.

In: CELL REP, Vol. 7, No. 3, 08.05.2014, p. 883-897.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Reverter, M, Rentero, C, Garcia-Melero, A, Hoque, M, Vilà de Muga, S, Alvarez-Guaita, A, Conway, JRW, Wood, P, Cairns, R, Lykopoulou, L, Grinberg, D, Vilageliu, L, Bosch, M, Heeren, J, Blasi, J, Timpson, P, Pol, A, Tebar, F, Murray, RZ, Grewal, T & Enrich, C 2014, 'Cholesterol Regulates Syntaxin 6 Trafficking at trans-Golgi Network Endosomal Boundaries', CELL REP, vol. 7, no. 3, pp. 883-897. https://doi.org/10.1016/j.celrep.2014.03.043

APA

Reverter, M., Rentero, C., Garcia-Melero, A., Hoque, M., Vilà de Muga, S., Alvarez-Guaita, A., Conway, J. R. W., Wood, P., Cairns, R., Lykopoulou, L., Grinberg, D., Vilageliu, L., Bosch, M., Heeren, J., Blasi, J., Timpson, P., Pol, A., Tebar, F., Murray, R. Z., ... Enrich, C. (2014). Cholesterol Regulates Syntaxin 6 Trafficking at trans-Golgi Network Endosomal Boundaries. CELL REP, 7(3), 883-897. https://doi.org/10.1016/j.celrep.2014.03.043

Vancouver

Reverter M, Rentero C, Garcia-Melero A, Hoque M, Vilà de Muga S, Alvarez-Guaita A et al. Cholesterol Regulates Syntaxin 6 Trafficking at trans-Golgi Network Endosomal Boundaries. CELL REP. 2014 May 8;7(3):883-897. https://doi.org/10.1016/j.celrep.2014.03.043

Bibtex

@article{cd79fc17ae4945fca4e7f7ea02a0254f,
title = "Cholesterol Regulates Syntaxin 6 Trafficking at trans-Golgi Network Endosomal Boundaries",
abstract = "Inhibition of cholesterol export from late endosomes causes cellular cholesterol imbalance, including cholesterol depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 mutant fibroblasts, we show that altered cholesterol levels at the TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, and Rab11-positive recycling endosomes (REs). This increases Stx6/VAMP3 interaction and interferes with the recycling of αVβ3 and α5β1 integrins and cell migration, possibly in a Stx6-dependent manner. In NPC1 mutant cells, restoration of cholesterol levels in the TGN, but not inhibition of VAMP3, restores the steady-state localization of Stx6 in the TGN. Furthermore, elevation of RE cholesterol is associated with increased amounts of Stx6 in RE. Hence, the fine-tuning of cholesterol levels at the TGN-RE boundaries together with a subset of cholesterol-sensitive SNARE proteins may play a regulatory role in cell migration and invasion.",
author = "Meritxell Reverter and Carles Rentero and Ana Garcia-Melero and Monira Hoque and {Vil{\`a} de Muga}, Sandra and Anna Alvarez-Guaita and Conway, {James R W} and Peta Wood and Rose Cairns and Lilia Lykopoulou and Daniel Grinberg and Llu{\"i}sa Vilageliu and Marta Bosch and Joerg Heeren and Juan Blasi and Paul Timpson and Albert Pol and Francesc Tebar and Murray, {Rachael Z} and Thomas Grewal and Carlos Enrich",
note = "Copyright {\textcopyright} 2014 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2014",
month = may,
day = "8",
doi = "10.1016/j.celrep.2014.03.043",
language = "English",
volume = "7",
pages = "883--897",
journal = "CELL REP",
issn = "2211-1247",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Cholesterol Regulates Syntaxin 6 Trafficking at trans-Golgi Network Endosomal Boundaries

AU - Reverter, Meritxell

AU - Rentero, Carles

AU - Garcia-Melero, Ana

AU - Hoque, Monira

AU - Vilà de Muga, Sandra

AU - Alvarez-Guaita, Anna

AU - Conway, James R W

AU - Wood, Peta

AU - Cairns, Rose

AU - Lykopoulou, Lilia

AU - Grinberg, Daniel

AU - Vilageliu, Lluïsa

AU - Bosch, Marta

AU - Heeren, Joerg

AU - Blasi, Juan

AU - Timpson, Paul

AU - Pol, Albert

AU - Tebar, Francesc

AU - Murray, Rachael Z

AU - Grewal, Thomas

AU - Enrich, Carlos

N1 - Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2014/5/8

Y1 - 2014/5/8

N2 - Inhibition of cholesterol export from late endosomes causes cellular cholesterol imbalance, including cholesterol depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 mutant fibroblasts, we show that altered cholesterol levels at the TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, and Rab11-positive recycling endosomes (REs). This increases Stx6/VAMP3 interaction and interferes with the recycling of αVβ3 and α5β1 integrins and cell migration, possibly in a Stx6-dependent manner. In NPC1 mutant cells, restoration of cholesterol levels in the TGN, but not inhibition of VAMP3, restores the steady-state localization of Stx6 in the TGN. Furthermore, elevation of RE cholesterol is associated with increased amounts of Stx6 in RE. Hence, the fine-tuning of cholesterol levels at the TGN-RE boundaries together with a subset of cholesterol-sensitive SNARE proteins may play a regulatory role in cell migration and invasion.

AB - Inhibition of cholesterol export from late endosomes causes cellular cholesterol imbalance, including cholesterol depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 mutant fibroblasts, we show that altered cholesterol levels at the TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, and Rab11-positive recycling endosomes (REs). This increases Stx6/VAMP3 interaction and interferes with the recycling of αVβ3 and α5β1 integrins and cell migration, possibly in a Stx6-dependent manner. In NPC1 mutant cells, restoration of cholesterol levels in the TGN, but not inhibition of VAMP3, restores the steady-state localization of Stx6 in the TGN. Furthermore, elevation of RE cholesterol is associated with increased amounts of Stx6 in RE. Hence, the fine-tuning of cholesterol levels at the TGN-RE boundaries together with a subset of cholesterol-sensitive SNARE proteins may play a regulatory role in cell migration and invasion.

U2 - 10.1016/j.celrep.2014.03.043

DO - 10.1016/j.celrep.2014.03.043

M3 - SCORING: Journal article

C2 - 24746815

VL - 7

SP - 883

EP - 897

JO - CELL REP

JF - CELL REP

SN - 2211-1247

IS - 3

ER -