Chitosan functionalized poly-epsilon-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications
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Chitosan functionalized poly-epsilon-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications. / Tardajos, Myriam G; Cama, Giuseppe; Dash, Mamoni; Misseeuw, Lara; Gheysens, Tom; Gorzelanny, Christian; Coenye, Tom; Dubruel, Peter.
In: CARBOHYD POLYM, Vol. 191, 01.07.2018, p. 127-135.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Chitosan functionalized poly-epsilon-caprolactone electrospun fibers and 3D printed scaffolds as antibacterial materials for tissue engineering applications
AU - Tardajos, Myriam G
AU - Cama, Giuseppe
AU - Dash, Mamoni
AU - Misseeuw, Lara
AU - Gheysens, Tom
AU - Gorzelanny, Christian
AU - Coenye, Tom
AU - Dubruel, Peter
N1 - Copyright © 2018 Elsevier Ltd. All rights reserved.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-ε-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw). High Mw chitosan exhibited a better coverage of the surface as indicated by the higher N% detected by X-ray photoelectron spectroscopy (XPS) and the observations with either scanning electron microscopy (SEM)(for fibers) or Coomassie blue staining (for 3D-printed scaffolds). A lactate dehydrogenase assay (LDH) using L929 fibroblasts demonstrated the cell-adhesion and cell-viability capacity of the modified samples. The antibacterial properties against S. aureus ATCC 6538 and S. epidermidis ET13 revealed a slower bacterial growth rate on the surface of the chitosan modified scaffolds, regardless the chitosan Mw.
AB - Tissue engineering (TE) approaches often employ polymer-based scaffolds to provide support with a view to the improved regeneration of damaged tissues. The aim of this research was to develop a surface modification method for introducing chitosan as an antibacterial agent in both electrospun membranes and 3D printed poly-ε-caprolactone (PCL) scaffolds. The scaffolds were functionalized by grafting methacrylic acid N-hydroxysuccinimide ester (NHSMA) onto the surface after Ar-plasma/air activation. Subsequently, the newly-introduced NHS groups were used to couple with chitosan of various molecular weights (Mw). High Mw chitosan exhibited a better coverage of the surface as indicated by the higher N% detected by X-ray photoelectron spectroscopy (XPS) and the observations with either scanning electron microscopy (SEM)(for fibers) or Coomassie blue staining (for 3D-printed scaffolds). A lactate dehydrogenase assay (LDH) using L929 fibroblasts demonstrated the cell-adhesion and cell-viability capacity of the modified samples. The antibacterial properties against S. aureus ATCC 6538 and S. epidermidis ET13 revealed a slower bacterial growth rate on the surface of the chitosan modified scaffolds, regardless the chitosan Mw.
KW - Journal Article
U2 - 10.1016/j.carbpol.2018.02.060
DO - 10.1016/j.carbpol.2018.02.060
M3 - SCORING: Journal article
C2 - 29661300
VL - 191
SP - 127
EP - 135
JO - CARBOHYD POLYM
JF - CARBOHYD POLYM
SN - 0144-8617
ER -
