Characterization of the murine T-lymphocyte response to Salmonella enterica serovar Typhimurium infection
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Characterization of the murine T-lymphocyte response to Salmonella enterica serovar Typhimurium infection. / Mittrücker, Hans-Willi; Köhler, Anne; Kaufmann, Stefan H E.
In: INFECT IMMUN, Vol. 70, No. 1, 01.01.2002, p. 199-203.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization of the murine T-lymphocyte response to Salmonella enterica serovar Typhimurium infection
AU - Mittrücker, Hans-Willi
AU - Köhler, Anne
AU - Kaufmann, Stefan H E
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Infection of mice with Salmonella enterica serotype Typhimurium induces a strong Th1 cell response that is central for the control of infection. We infected mice of a resistant background with a virulent strain of S. enterica serovar Typhimurium and analyzed the kinetics and magnitude of the T-cell response. After infection, the majority of CD4(+) and CD8(+) splenocytes acquired an activated phenotype, as indicated by expression levels of CD44 and CD62L. In addition, after 3 to 4 weeks of infection, more than 20% of the CD4(+) and more than 30% of the CD8(+) T cells produced gamma interferon (IFN-gamma) in response to short-term polyclonal stimulation. In contrast, we detected only a moderate (two- to threefold) expansion of both T-cell populations, and BrdU incorporation revealed that there was either no or only a limited increase in the in vivo proliferation of CD4(+) and CD8(+) T cells, respectively. Our results indicate that although an unexpectedly large population of both CD4(+) and CD8(+) T cells is activated and acquires the potential to secrete IFN-gamma, this activation is not paralleled by substantial expansion of these T-cell populations.
AB - Infection of mice with Salmonella enterica serotype Typhimurium induces a strong Th1 cell response that is central for the control of infection. We infected mice of a resistant background with a virulent strain of S. enterica serovar Typhimurium and analyzed the kinetics and magnitude of the T-cell response. After infection, the majority of CD4(+) and CD8(+) splenocytes acquired an activated phenotype, as indicated by expression levels of CD44 and CD62L. In addition, after 3 to 4 weeks of infection, more than 20% of the CD4(+) and more than 30% of the CD8(+) T cells produced gamma interferon (IFN-gamma) in response to short-term polyclonal stimulation. In contrast, we detected only a moderate (two- to threefold) expansion of both T-cell populations, and BrdU incorporation revealed that there was either no or only a limited increase in the in vivo proliferation of CD4(+) and CD8(+) T cells, respectively. Our results indicate that although an unexpectedly large population of both CD4(+) and CD8(+) T cells is activated and acquires the potential to secrete IFN-gamma, this activation is not paralleled by substantial expansion of these T-cell populations.
KW - Animals
KW - CD4-Positive T-Lymphocytes
KW - CD8-Positive T-Lymphocytes
KW - Cell Division
KW - Cells, Cultured
KW - Disease Models, Animal
KW - Female
KW - Immunophenotyping
KW - Interferon-gamma
KW - Mice
KW - Mice, Inbred C57BL
KW - Salmonella Infections
KW - Salmonella typhimurium
M3 - SCORING: Journal article
C2 - 11748183
VL - 70
SP - 199
EP - 203
JO - INFECT IMMUN
JF - INFECT IMMUN
SN - 0019-9567
IS - 1
ER -