Characterization of the H-kininogen-binding site on factor XI

TY - JOUR

T1 - Characterization of the H-kininogen-binding site on factor XI

T2 - a comparison of factor XI and plasma prekallikrein

AU - Renné, Thomas

AU - Gailani, David

AU - Meijers, Joost C M

AU - Müller-Esterl, Werner

PY - 2002/2/15

Y1 - 2002/2/15

N2 - Factor XI (FXI), the zymogen of the blood coagulation protease FXIa, and the structurally homologous protein plasma prekallikrein circulate in plasma in noncovalent complexes with H-kininogen (HK). HK binds to the heavy chains of FXI and of prekallikrein. Each chain contains four apple domains (F1-F4 for FXI and P1-P4 for prekallikrein). Previous studies indicated that the HK-binding site on FXI is located in F1, whereas the major HK-binding site on prekallikrein is in P2. To determine the contribution of each FXI apple domain to HK-FXI complex formation, we examined binding of recombinant single apple domain-tissue plasminogen activator fusion proteins to HK. The order of affinity from highest to lowest is F2 F4 > F1 F3. Monoclonal antibodies against F2 are superior to F4 or F1 antibodies as inhibitors of HK binding to FXI. Antibody alphaP2, raised against prekallikrein, cross-reacts with FXI F2 and inhibits FXI-HK binding with an IC(50) of 8 nm. HK binding to a platelet-specific FXI variant lacking the N-terminal half of F2 is reduced > 5-fold compared with full-length FXI. A chimeric FXI molecule in which F2 is replaced by P2 is cleaved within P2 during activation by factor XIIa, resulting in greatly reduced HK binding capacity. In contrast, wild-type FXI is not cleaved within F2, and its binding capacity for HK is unaffected by factor XIIa. Our data show that HK binding to FXI involves multiple apple domains, with F2 being most important. The findings demonstrate a similarity in mechanism for FXI and prekallikrein binding to HK.

AB - Factor XI (FXI), the zymogen of the blood coagulation protease FXIa, and the structurally homologous protein plasma prekallikrein circulate in plasma in noncovalent complexes with H-kininogen (HK). HK binds to the heavy chains of FXI and of prekallikrein. Each chain contains four apple domains (F1-F4 for FXI and P1-P4 for prekallikrein). Previous studies indicated that the HK-binding site on FXI is located in F1, whereas the major HK-binding site on prekallikrein is in P2. To determine the contribution of each FXI apple domain to HK-FXI complex formation, we examined binding of recombinant single apple domain-tissue plasminogen activator fusion proteins to HK. The order of affinity from highest to lowest is F2 F4 > F1 F3. Monoclonal antibodies against F2 are superior to F4 or F1 antibodies as inhibitors of HK binding to FXI. Antibody alphaP2, raised against prekallikrein, cross-reacts with FXI F2 and inhibits FXI-HK binding with an IC(50) of 8 nm. HK binding to a platelet-specific FXI variant lacking the N-terminal half of F2 is reduced > 5-fold compared with full-length FXI. A chimeric FXI molecule in which F2 is replaced by P2 is cleaved within P2 during activation by factor XIIa, resulting in greatly reduced HK binding capacity. In contrast, wild-type FXI is not cleaved within F2, and its binding capacity for HK is unaffected by factor XIIa. Our data show that HK binding to FXI involves multiple apple domains, with F2 being most important. The findings demonstrate a similarity in mechanism for FXI and prekallikrein binding to HK.

KW - Alternative Splicing

KW - Animals

KW - Binding Sites

KW - Blotting, Western

KW - Cell Line

KW - Cricetinae

KW - Dose-Response Relationship, Drug

KW - Electrophoresis, Polyacrylamide Gel

KW - Enzyme-Linked Immunosorbent Assay

KW - Factor XI

KW - Humans

KW - Inhibitory Concentration 50

KW - Kininogen, High-Molecular-Weight

KW - Precipitin Tests

KW - Prekallikrein

KW - Protein Binding

KW - Protein Structure, Tertiary

KW - Recombinant Fusion Proteins

U2 - 10.1074/jbc.M105221200

DO - 10.1074/jbc.M105221200

M3 - SCORING: Journal article

C2 - 11733491

VL - 277

SP - 4892

EP - 4899

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 7

ER -