Characterization of Developmental Trajectories of Dendritic Cell Hematopoiesis Through Single-Cell RNA Sequencing Methods
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Abstract
Single-cell technologies have become valuable tools to trace dendritic cell differentiation trajectories. Here, we illustrate the workflow used for processing of mouse bone marrow for single-cell RNA sequencing and trajectory analyses, as done in Dress et al. (Nat Immunol 20:852-864, 2019). This short methodology is presented as a starting point for researchers just beginning to dive into the complex field of dendritic cell ontogeny and cellular development trajectory analyses.
Bibliographical data
Original language | English |
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Title of host publication | Dendritic Cells : Methods and Protocols |
Editors | Vanja Sisirak |
REQUIRED books only: Number of pages | 11 |
Place of Publication | New York, NY |
Publisher | Humana Press |
Publication date | 12.03.2023 |
Edition | 1 |
Pages | 375-385 |
ISBN (Print) | 978-1-0716-2937-6 |
ISBN (Electronic) | 978-1-0716-2938-3 |
DOIs | |
Publication status | Published - 12.03.2023 |
Comment Deanary
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PubMed | 36905527 |
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