Characterization and Distribution of Reelin-Positive Interneuron Subtypes in the Rat Barrel Cortex
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Characterization and Distribution of Reelin-Positive Interneuron Subtypes in the Rat Barrel Cortex. / Pohlkamp, Theresa; Dávid, Csaba; Cauli, Bruno; Gallopin, Thierry; Bouché, Elisabeth; Karagiannis, Anastassios; May, Petra; Herz, Joachim; Frotscher, Michael; Staiger, Jochen F; Bock, Hans H.
In: CEREB CORTEX, Vol. 24, No. 11, 2014, p. 3046-3058.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization and Distribution of Reelin-Positive Interneuron Subtypes in the Rat Barrel Cortex
AU - Pohlkamp, Theresa
AU - Dávid, Csaba
AU - Cauli, Bruno
AU - Gallopin, Thierry
AU - Bouché, Elisabeth
AU - Karagiannis, Anastassios
AU - May, Petra
AU - Herz, Joachim
AU - Frotscher, Michael
AU - Staiger, Jochen F
AU - Bock, Hans H
PY - 2014
Y1 - 2014
N2 - GABAergic inhibitory interneurons (IN) represent a heterogeneous population with different electrophysiological, morphological, and molecular properties. The correct balance between interneuronal subtypes is important for brain function and is impaired in several neurological and psychiatric disorders. Here we show the data of 123 molecularly and electrophysiologically characterized neurons of juvenile rat barrel cortex acute slices, 48 of which expressed Reelin (Reln). Reln mRNA was exclusively detected in Gad65/67-positive cells but was found in interneuronal subtypes in different proportions: all cells of the adapting-Somatostatin (SST) cluster expressed Reln, whereas 63% of the adapting-neuropeptide Y (NPY, 50% of the fast-spiking Parvalbumin (PVALB), and 27% of the adapting/bursting-Vasoactive Intestinal Peptide (VIP) cluster were Reln-positive. Silhouette analysis revealed a high impact of the parameter Reln on cluster quality. By analyzing the co-localization of RELN immunoreactivity with those of different IN-markers, we found that RELN is produced layer-independently in SST-, NPY-, and NOS1-expressing INs, whereas co-localization of RELN and VIP was mostly absent. Of note, RELN co-localized with PVALB, predominantly in INs of layers IV/V (>30%). Our findings emphasize RELN's role as an important IN-marker protein and provide a basis for the functional characterization of Reln-expressing INs and its role in the regulation of inhibitory IN networks.
AB - GABAergic inhibitory interneurons (IN) represent a heterogeneous population with different electrophysiological, morphological, and molecular properties. The correct balance between interneuronal subtypes is important for brain function and is impaired in several neurological and psychiatric disorders. Here we show the data of 123 molecularly and electrophysiologically characterized neurons of juvenile rat barrel cortex acute slices, 48 of which expressed Reelin (Reln). Reln mRNA was exclusively detected in Gad65/67-positive cells but was found in interneuronal subtypes in different proportions: all cells of the adapting-Somatostatin (SST) cluster expressed Reln, whereas 63% of the adapting-neuropeptide Y (NPY, 50% of the fast-spiking Parvalbumin (PVALB), and 27% of the adapting/bursting-Vasoactive Intestinal Peptide (VIP) cluster were Reln-positive. Silhouette analysis revealed a high impact of the parameter Reln on cluster quality. By analyzing the co-localization of RELN immunoreactivity with those of different IN-markers, we found that RELN is produced layer-independently in SST-, NPY-, and NOS1-expressing INs, whereas co-localization of RELN and VIP was mostly absent. Of note, RELN co-localized with PVALB, predominantly in INs of layers IV/V (>30%). Our findings emphasize RELN's role as an important IN-marker protein and provide a basis for the functional characterization of Reln-expressing INs and its role in the regulation of inhibitory IN networks.
U2 - 10.1093/cercor/bht161
DO - 10.1093/cercor/bht161
M3 - SCORING: Journal article
C2 - 23803971
VL - 24
SP - 3046
EP - 3058
JO - CEREB CORTEX
JF - CEREB CORTEX
SN - 1047-3211
IS - 11
ER -