Research ExplorerPublicationsChanges in proinflammatory gene expression in human whole bl...

Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces

Standard

Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces. / Harder, Sönke; Quabius, Elgar Susanne; Meinke, Fabian; Mehl, Christian; Kern, Matthias.

In: CLIN ORAL INVEST, Vol. 23, No. 10, 10.2019, p. 3731-3738.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Harder, S, Quabius, ES, Meinke, F, Mehl, C & Kern, M 2019, 'Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces', CLIN ORAL INVEST, vol. 23, no. 10, pp. 3731-3738. https://doi.org/10.1007/s00784-019-02801-7

APA

Harder, S., Quabius, E. S., Meinke, F., Mehl, C., & Kern, M. (2019). Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces. CLIN ORAL INVEST, 23(10), 3731-3738. https://doi.org/10.1007/s00784-019-02801-7

Vancouver

Harder S, Quabius ES, Meinke F, Mehl C, Kern M. Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces. CLIN ORAL INVEST. 2019 Oct;23(10):3731-3738. https://doi.org/10.1007/s00784-019-02801-7

Bibtex

@article{05e3cf4441f44eb2a0392fb58bb46bf3,
title = "Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces",
abstract = "OBJECTIVES: The aim of this in vitro study was to assess changes in the gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces conditioned by UV light. To this end, expression levels of proinflammatory cytokines were analyzed in vitro in human whole blood.MATERIALS AND METHODS: Dental implants made of grade 4 titanium were conditioned by UV light in a UV device and submerged in human whole blood. Unconditioned implants served as controls, and blood samples without implants served as the negative control group. Sampling was performed at 1, 8, and 24 h. Changes in the expression levels of interleukin-1β (IL1B) and tumor necrosis factor alpha (TNF) were assessed using RT-qPCR at the mRNA level.RESULTS: The gene expression of IL1B was significantly suppressed in the test group over the observation period compared to the control group during the 1-8 h after having contact between the implant surface and the blood. The gene expression of TNF was not significantly altered by UV conditioning after 1 and 8 h of observation, but both cytokine expression levels were increased significantly after 24 h.CONCLUSIONS: Differences in the gene expression of proinflammatory cytokines after insertion of UV-conditioned titanium implants can be assessed using a human whole blood test. UV-conditioned implant surfaces apparently suppress the release of IL1B in vitro.CLINICAL RELEVANCE: The results of our publication demonstrate that modulation of the early inflammatory response in human whole blood is possible by surface treatment with UV light. In particular, the suppression of IL1B expression, especially after the initial contact of blood cells, may be beneficial in the osseointegration of titanium implants by positively influence the balance between rejection and acceptance of an implant.",
keywords = "Blood, Dental Implants, Gene Expression, Humans, Inflammation, Interleukin-1beta/blood, Osseointegration, Surface Properties, Titanium, Tumor Necrosis Factor-alpha/blood, Ultraviolet Rays",
author = "S{\"o}nke Harder and Quabius, {Elgar Susanne} and Fabian Meinke and Christian Mehl and Matthias Kern",
year = "2019",
month = oct,
doi = "10.1007/s00784-019-02801-7",
language = "English",
volume = "23",
pages = "3731--3738",
journal = "CLIN ORAL INVEST",
issn = "1432-6981",
publisher = "Springer",
number = "10",

}

RIS

TY - JOUR

T1 - Changes in proinflammatory gene expression in human whole blood after contact with UV-conditioned implant surfaces

AU - Harder, Sönke

AU - Quabius, Elgar Susanne

AU - Meinke, Fabian

AU - Mehl, Christian

AU - Kern, Matthias

PY - 2019/10

Y1 - 2019/10

N2 - OBJECTIVES: The aim of this in vitro study was to assess changes in the gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces conditioned by UV light. To this end, expression levels of proinflammatory cytokines were analyzed in vitro in human whole blood.MATERIALS AND METHODS: Dental implants made of grade 4 titanium were conditioned by UV light in a UV device and submerged in human whole blood. Unconditioned implants served as controls, and blood samples without implants served as the negative control group. Sampling was performed at 1, 8, and 24 h. Changes in the expression levels of interleukin-1β (IL1B) and tumor necrosis factor alpha (TNF) were assessed using RT-qPCR at the mRNA level.RESULTS: The gene expression of IL1B was significantly suppressed in the test group over the observation period compared to the control group during the 1-8 h after having contact between the implant surface and the blood. The gene expression of TNF was not significantly altered by UV conditioning after 1 and 8 h of observation, but both cytokine expression levels were increased significantly after 24 h.CONCLUSIONS: Differences in the gene expression of proinflammatory cytokines after insertion of UV-conditioned titanium implants can be assessed using a human whole blood test. UV-conditioned implant surfaces apparently suppress the release of IL1B in vitro.CLINICAL RELEVANCE: The results of our publication demonstrate that modulation of the early inflammatory response in human whole blood is possible by surface treatment with UV light. In particular, the suppression of IL1B expression, especially after the initial contact of blood cells, may be beneficial in the osseointegration of titanium implants by positively influence the balance between rejection and acceptance of an implant.

AB - OBJECTIVES: The aim of this in vitro study was to assess changes in the gene expression of proinflammatory cytokines in human whole blood after contact with titanium implant surfaces conditioned by UV light. To this end, expression levels of proinflammatory cytokines were analyzed in vitro in human whole blood.MATERIALS AND METHODS: Dental implants made of grade 4 titanium were conditioned by UV light in a UV device and submerged in human whole blood. Unconditioned implants served as controls, and blood samples without implants served as the negative control group. Sampling was performed at 1, 8, and 24 h. Changes in the expression levels of interleukin-1β (IL1B) and tumor necrosis factor alpha (TNF) were assessed using RT-qPCR at the mRNA level.RESULTS: The gene expression of IL1B was significantly suppressed in the test group over the observation period compared to the control group during the 1-8 h after having contact between the implant surface and the blood. The gene expression of TNF was not significantly altered by UV conditioning after 1 and 8 h of observation, but both cytokine expression levels were increased significantly after 24 h.CONCLUSIONS: Differences in the gene expression of proinflammatory cytokines after insertion of UV-conditioned titanium implants can be assessed using a human whole blood test. UV-conditioned implant surfaces apparently suppress the release of IL1B in vitro.CLINICAL RELEVANCE: The results of our publication demonstrate that modulation of the early inflammatory response in human whole blood is possible by surface treatment with UV light. In particular, the suppression of IL1B expression, especially after the initial contact of blood cells, may be beneficial in the osseointegration of titanium implants by positively influence the balance between rejection and acceptance of an implant.

KW - Blood

KW - Dental Implants

KW - Gene Expression

KW - Humans

KW - Inflammation

KW - Interleukin-1beta/blood

KW - Osseointegration

KW - Surface Properties

KW - Titanium

KW - Tumor Necrosis Factor-alpha/blood

KW - Ultraviolet Rays

U2 - 10.1007/s00784-019-02801-7

DO - 10.1007/s00784-019-02801-7

M3 - SCORING: Journal article

C2 - 30666479

VL - 23

SP - 3731

EP - 3738

JO - CLIN ORAL INVEST

JF - CLIN ORAL INVEST

SN - 1432-6981

IS - 10

ER -