Research ExplorerPublicationsCellular Responses to Strong Overexpression of Recombinant G...

Cellular Responses to Strong Overexpression of Recombinant Genes in Escherichia Coli

Standard

Cellular Responses to Strong Overexpression of Recombinant Genes in Escherichia Coli. / Lin, Hongying; Hanschke, Renate; Nicklisch, Silke; Riemschneider, Stephan; Meyer, Sylke; Gupta, Antje; Neubauer, Peter.

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology: Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th–8th October 2000. Springer Netherlands, 2001.

Research output: SCORING: Contribution to book/anthology › Contribution to scientific report › Research

Harvard

Lin, H, Hanschke, R, Nicklisch, S, Riemschneider, S, Meyer, S, Gupta, A & Neubauer, P 2001, Cellular Responses to Strong Overexpression of Recombinant Genes in Escherichia Coli. in Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology: Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th–8th October 2000. Springer Netherlands. https://doi.org/10.1007/978-94-015-9749-4_5

APA

Lin, H., Hanschke, R., Nicklisch, S., Riemschneider, S., Meyer, S., Gupta, A., & Neubauer, P. (2001). Cellular Responses to Strong Overexpression of Recombinant Genes in Escherichia Coli. In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology: Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th–8th October 2000 Springer Netherlands. https://doi.org/10.1007/978-94-015-9749-4_5

Vancouver

Lin H, Hanschke R, Nicklisch S, Riemschneider S, Meyer S, Gupta A et al. Cellular Responses to Strong Overexpression of Recombinant Genes in Escherichia Coli. In Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology: Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th–8th October 2000. Springer Netherlands. 2001 https://doi.org/10.1007/978-94-015-9749-4_5

Bibtex

@inbook{fc75aaa46b6843d1857c4aeb729db291,
title = "Cellular Responses to Strong Overexpression of Recombinant Genes in Escherichia Coli",
abstract = "Changes in the physiology of Escherichia coli cells producing a recombinant α-glucosidase were studied in glucose limited fed-batch fermentations. High α-glucosidase formation is connected to growth inhibition and loss of culturability. Although some metabolic functions are maintained, the ability for replication is apparently not only impaired by competition of recombinant product synthesis to the formation of cellular house-keeping proteins, but is related to continued damage of the chromosomal DNA, which is concluded from electron microscopical analysis and from the behaviour of the SOS response repressor protein LexA. Although, from the decrease of LexA, we propose an SOS signal, the cells are unable to induce the SOS response, due to the high synthesis of α-glucosidase and the concurrent inhibition of the protein synthesis system.",
author = "Hongying Lin and Renate Hanschke and Silke Nicklisch and Stephan Riemschneider and Sylke Meyer and Antje Gupta and Peter Neubauer",
year = "2001",
doi = "10.1007/978-94-015-9749-4_5",
language = "English",
isbn = "978-90-481-5756-3",
booktitle = "Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology",
publisher = "Springer Netherlands",
address = "Netherlands",

}

RIS

TY - CHAP

T1 - Cellular Responses to Strong Overexpression of Recombinant Genes in Escherichia Coli

AU - Lin, Hongying

AU - Hanschke, Renate

AU - Nicklisch, Silke

AU - Riemschneider, Stephan

AU - Meyer, Sylke

AU - Gupta, Antje

AU - Neubauer, Peter

PY - 2001

Y1 - 2001

N2 - Changes in the physiology of Escherichia coli cells producing a recombinant α-glucosidase were studied in glucose limited fed-batch fermentations. High α-glucosidase formation is connected to growth inhibition and loss of culturability. Although some metabolic functions are maintained, the ability for replication is apparently not only impaired by competition of recombinant product synthesis to the formation of cellular house-keeping proteins, but is related to continued damage of the chromosomal DNA, which is concluded from electron microscopical analysis and from the behaviour of the SOS response repressor protein LexA. Although, from the decrease of LexA, we propose an SOS signal, the cells are unable to induce the SOS response, due to the high synthesis of α-glucosidase and the concurrent inhibition of the protein synthesis system.

AB - Changes in the physiology of Escherichia coli cells producing a recombinant α-glucosidase were studied in glucose limited fed-batch fermentations. High α-glucosidase formation is connected to growth inhibition and loss of culturability. Although some metabolic functions are maintained, the ability for replication is apparently not only impaired by competition of recombinant product synthesis to the formation of cellular house-keeping proteins, but is related to continued damage of the chromosomal DNA, which is concluded from electron microscopical analysis and from the behaviour of the SOS response repressor protein LexA. Although, from the decrease of LexA, we propose an SOS signal, the cells are unable to induce the SOS response, due to the high synthesis of α-glucosidase and the concurrent inhibition of the protein synthesis system.

U2 - 10.1007/978-94-015-9749-4_5

DO - 10.1007/978-94-015-9749-4_5

M3 - Contribution to scientific report

SN - 978-90-481-5756-3

BT - Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

PB - Springer Netherlands

ER -