Research ExplorerPublicationsCellular and Molecular Probing of Intact Human Organs

Cellular and Molecular Probing of Intact Human Organs

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Cellular and Molecular Probing of Intact Human Organs. / Zhao, Shan; Todorov, Mihail Ivilinov; Cai, Ruiyao; -Maskari, Rami Ai; Steinke, Hanno; Kemter, Elisabeth; Mai, Hongcheng; Rong, Zhouyi; Warmer, Martin; Stanic, Karen; Schoppe, Oliver; Paetzold, Johannes Christian; Gesierich, Benno; Wong, Milagros N; Huber, Tobias B; Duering, Marco; Bruns, Oliver Thomas; Menze, Bjoern; Lipfert, Jan; Puelles, Victor G; Wolf, Eckhard; Bechmann, Ingo; Ertürk, Ali.

In: CELL, Vol. 180, No. 4, 20.02.2020, p. 796-812.e19.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Zhao, S, Todorov, MI, Cai, R, -Maskari, RA, Steinke, H, Kemter, E, Mai, H, Rong, Z, Warmer, M, Stanic, K, Schoppe, O, Paetzold, JC, Gesierich, B, Wong, MN, Huber, TB, Duering, M, Bruns, OT, Menze, B, Lipfert, J, Puelles, VG, Wolf, E, Bechmann, I & Ertürk, A 2020, 'Cellular and Molecular Probing of Intact Human Organs', CELL, vol. 180, no. 4, pp. 796-812.e19. https://doi.org/10.1016/j.cell.2020.01.030

APA

Zhao, S., Todorov, M. I., Cai, R., -Maskari, R. A., Steinke, H., Kemter, E., Mai, H., Rong, Z., Warmer, M., Stanic, K., Schoppe, O., Paetzold, J. C., Gesierich, B., Wong, M. N., Huber, T. B., Duering, M., Bruns, O. T., Menze, B., Lipfert, J., ... Ertürk, A. (2020). Cellular and Molecular Probing of Intact Human Organs. CELL, 180(4), 796-812.e19. https://doi.org/10.1016/j.cell.2020.01.030

Vancouver

Zhao S, Todorov MI, Cai R, -Maskari RA, Steinke H, Kemter E et al. Cellular and Molecular Probing of Intact Human Organs. CELL. 2020 Feb 20;180(4):796-812.e19. https://doi.org/10.1016/j.cell.2020.01.030

Bibtex

@article{3c337432f8cb49499ab639c1609803fe,
title = "Cellular and Molecular Probing of Intact Human Organs",
abstract = "Optical tissue transparency permits scalable cellular and molecular investigation of complex tissues in 3D. Adult human organs are particularly challenging to render transparent because of the accumulation of dense and sturdy molecules in decades-aged tissues. To overcome these challenges, we developed SHANEL, a method based on a new tissue permeabilization approach to clear and label stiff human organs. We used SHANEL to render the intact adult human brain and kidney transparent and perform 3D histology with antibodies and dyes in centimeters-depth. Thereby, we revealed structural details of the intact human eye, human thyroid, human kidney, and transgenic pig pancreas at the cellular resolution. Furthermore, we developed a deep learning pipeline to analyze millions of cells in cleared human brain tissues within hours with standard lab computers. Overall, SHANEL is a robust and unbiased technology to chart the cellular and molecular architecture of large intact mammalian organs.",
author = "Shan Zhao and Todorov, {Mihail Ivilinov} and Ruiyao Cai and -Maskari, {Rami Ai} and Hanno Steinke and Elisabeth Kemter and Hongcheng Mai and Zhouyi Rong and Martin Warmer and Karen Stanic and Oliver Schoppe and Paetzold, {Johannes Christian} and Benno Gesierich and Wong, {Milagros N} and Huber, {Tobias B} and Marco Duering and Bruns, {Oliver Thomas} and Bjoern Menze and Jan Lipfert and Puelles, {Victor G} and Eckhard Wolf and Ingo Bechmann and Ali Ert{\"u}rk",
note = "Copyright {\textcopyright} 2020 Elsevier Inc. All rights reserved.",
year = "2020",
month = feb,
day = "20",
doi = "10.1016/j.cell.2020.01.030",
language = "English",
volume = "180",
pages = "796--812.e19",
journal = "CELL",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Cellular and Molecular Probing of Intact Human Organs

AU - Zhao, Shan

AU - Todorov, Mihail Ivilinov

AU - Cai, Ruiyao

AU - -Maskari, Rami Ai

AU - Steinke, Hanno

AU - Kemter, Elisabeth

AU - Mai, Hongcheng

AU - Rong, Zhouyi

AU - Warmer, Martin

AU - Stanic, Karen

AU - Schoppe, Oliver

AU - Paetzold, Johannes Christian

AU - Gesierich, Benno

AU - Wong, Milagros N

AU - Huber, Tobias B

AU - Duering, Marco

AU - Bruns, Oliver Thomas

AU - Menze, Bjoern

AU - Lipfert, Jan

AU - Puelles, Victor G

AU - Wolf, Eckhard

AU - Bechmann, Ingo

AU - Ertürk, Ali

N1 - Copyright © 2020 Elsevier Inc. All rights reserved.

PY - 2020/2/20

Y1 - 2020/2/20

N2 - Optical tissue transparency permits scalable cellular and molecular investigation of complex tissues in 3D. Adult human organs are particularly challenging to render transparent because of the accumulation of dense and sturdy molecules in decades-aged tissues. To overcome these challenges, we developed SHANEL, a method based on a new tissue permeabilization approach to clear and label stiff human organs. We used SHANEL to render the intact adult human brain and kidney transparent and perform 3D histology with antibodies and dyes in centimeters-depth. Thereby, we revealed structural details of the intact human eye, human thyroid, human kidney, and transgenic pig pancreas at the cellular resolution. Furthermore, we developed a deep learning pipeline to analyze millions of cells in cleared human brain tissues within hours with standard lab computers. Overall, SHANEL is a robust and unbiased technology to chart the cellular and molecular architecture of large intact mammalian organs.

AB - Optical tissue transparency permits scalable cellular and molecular investigation of complex tissues in 3D. Adult human organs are particularly challenging to render transparent because of the accumulation of dense and sturdy molecules in decades-aged tissues. To overcome these challenges, we developed SHANEL, a method based on a new tissue permeabilization approach to clear and label stiff human organs. We used SHANEL to render the intact adult human brain and kidney transparent and perform 3D histology with antibodies and dyes in centimeters-depth. Thereby, we revealed structural details of the intact human eye, human thyroid, human kidney, and transgenic pig pancreas at the cellular resolution. Furthermore, we developed a deep learning pipeline to analyze millions of cells in cleared human brain tissues within hours with standard lab computers. Overall, SHANEL is a robust and unbiased technology to chart the cellular and molecular architecture of large intact mammalian organs.

U2 - 10.1016/j.cell.2020.01.030

DO - 10.1016/j.cell.2020.01.030

M3 - SCORING: Journal article

C2 - 32059778

VL - 180

SP - 796-812.e19

JO - CELL

JF - CELL

SN - 0092-8674

IS - 4

ER -