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CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas

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CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas. / Uhlig, Ria; Bröker, Nina; Weidemann, Sören; Gorbokon, Natalia; Menz, Anne; Büscheck, Franziska; Luebke, Andreas M; Putri, Devita; Kluth, Martina; Hube-Magg, Claudia; Hinsch, Andrea; Lennartz, Maximilian; Reiswich, Viktor; Höflmayer, Doris; Fraune, Christoph; Möller, Katharina; Bernreuther, Christian; Lebok, Patrick; Sauter, Guido; Minner, Sarah; Steurer, Stefan; Burandt, Eike; Krech, Rainer; Dum, David; Marx, Andreas; Simon, Ronald; Krech, Till; Clauditz, Till S; Jacobsen, Frank.

In: PLOS ONE, Vol. 18, No. 6, 2023, p. e0287528.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Uhlig, R, Bröker, N, Weidemann, S, Gorbokon, N, Menz, A, Büscheck, F, Luebke, AM, Putri, D, Kluth, M, Hube-Magg, C, Hinsch, A, Lennartz, M, Reiswich, V, Höflmayer, D, Fraune, C, Möller, K, Bernreuther, C, Lebok, P, Sauter, G, Minner, S, Steurer, S, Burandt, E, Krech, R, Dum, D, Marx, A, Simon, R, Krech, T, Clauditz, TS & Jacobsen, F 2023, 'CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas', PLOS ONE, vol. 18, no. 6, pp. e0287528. https://doi.org/10.1371/journal.pone.0287528

APA

Uhlig, R., Bröker, N., Weidemann, S., Gorbokon, N., Menz, A., Büscheck, F., Luebke, A. M., Putri, D., Kluth, M., Hube-Magg, C., Hinsch, A., Lennartz, M., Reiswich, V., Höflmayer, D., Fraune, C., Möller, K., Bernreuther, C., Lebok, P., Sauter, G., ... Jacobsen, F. (2023). CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas. PLOS ONE, 18(6), e0287528. https://doi.org/10.1371/journal.pone.0287528

Vancouver

Uhlig R, Bröker N, Weidemann S, Gorbokon N, Menz A, Büscheck F et al. CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas. PLOS ONE. 2023;18(6):e0287528. https://doi.org/10.1371/journal.pone.0287528

Bibtex

@article{38195e6e62814173b4754e192f116171,
title = "CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas",
abstract = "Chymotrypsin-like elastase family member 3B (CELA3B, elastase-3B) is a pancreatic enzyme with digestive function in the intestine. Since RNA analyses of normal tissues suggest that CELA3B expression is limited to the pancreas, the potential diagnostic utility of CELA3B immunohistochemistry for the distinction of pancreatic from extrapancreatic cancers and in the distinction of acinar cell carcinoma from ductal adenocarcinoma was assessed. CELA3B expression was successfully analyzed in 13,223 tumor samples from 132 different tumor types and subtypes as well as 8 samples each of 76 different normal tissue types by immunohistochemistry in a tissue microarray format (TMA). In normal tissues, CELA3B immunostaining was only seen in acinar cells and in a fraction of ductal cells of the pancreas as well as on some apical membranes of surface epithelial cells of the intestine. Among tumors, CELA3B immunostaining was seen in 12 of 16 (75%) acinar cell carcinoma of the pancreas including 6 cases with strong staining (37.5%) as well as in 5 of 13,207 other tumors (0.04%). These included 1.2% of 91 adenoid cystic carcinomas, 1.2% of 246 mucoepidermoid carcinomas and 0.8% of 127 acinic cell carcinomas of salivary glands. Our data show a good sensitivity (75%) and a high specificity (99.9%) of CELA3B immunohistochemistry for diagnosing acinar cell carcinoma of the pancreas.",
keywords = "Humans, Carcinoma, Acinar Cell/diagnosis, Pancreas/pathology, Salivary Glands/metabolism, Carcinoma, Adenoid Cystic/metabolism, Pancreatic Elastase/metabolism, Biomarkers, Tumor/metabolism",
author = "Ria Uhlig and Nina Br{\"o}ker and S{\"o}ren Weidemann and Natalia Gorbokon and Anne Menz and Franziska B{\"u}scheck and Luebke, {Andreas M} and Devita Putri and Martina Kluth and Claudia Hube-Magg and Andrea Hinsch and Maximilian Lennartz and Viktor Reiswich and Doris H{\"o}flmayer and Christoph Fraune and Katharina M{\"o}ller and Christian Bernreuther and Patrick Lebok and Guido Sauter and Sarah Minner and Stefan Steurer and Eike Burandt and Rainer Krech and David Dum and Andreas Marx and Ronald Simon and Till Krech and Clauditz, {Till S} and Frank Jacobsen",
note = "Copyright: {\textcopyright} 2023 Uhlig et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2023",
doi = "10.1371/journal.pone.0287528",
language = "English",
volume = "18",
pages = "e0287528",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas

AU - Uhlig, Ria

AU - Bröker, Nina

AU - Weidemann, Sören

AU - Gorbokon, Natalia

AU - Menz, Anne

AU - Büscheck, Franziska

AU - Luebke, Andreas M

AU - Putri, Devita

AU - Kluth, Martina

AU - Hube-Magg, Claudia

AU - Hinsch, Andrea

AU - Lennartz, Maximilian

AU - Reiswich, Viktor

AU - Höflmayer, Doris

AU - Fraune, Christoph

AU - Möller, Katharina

AU - Bernreuther, Christian

AU - Lebok, Patrick

AU - Sauter, Guido

AU - Minner, Sarah

AU - Steurer, Stefan

AU - Burandt, Eike

AU - Krech, Rainer

AU - Dum, David

AU - Marx, Andreas

AU - Simon, Ronald

AU - Krech, Till

AU - Clauditz, Till S

AU - Jacobsen, Frank

N1 - Copyright: © 2023 Uhlig et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2023

Y1 - 2023

N2 - Chymotrypsin-like elastase family member 3B (CELA3B, elastase-3B) is a pancreatic enzyme with digestive function in the intestine. Since RNA analyses of normal tissues suggest that CELA3B expression is limited to the pancreas, the potential diagnostic utility of CELA3B immunohistochemistry for the distinction of pancreatic from extrapancreatic cancers and in the distinction of acinar cell carcinoma from ductal adenocarcinoma was assessed. CELA3B expression was successfully analyzed in 13,223 tumor samples from 132 different tumor types and subtypes as well as 8 samples each of 76 different normal tissue types by immunohistochemistry in a tissue microarray format (TMA). In normal tissues, CELA3B immunostaining was only seen in acinar cells and in a fraction of ductal cells of the pancreas as well as on some apical membranes of surface epithelial cells of the intestine. Among tumors, CELA3B immunostaining was seen in 12 of 16 (75%) acinar cell carcinoma of the pancreas including 6 cases with strong staining (37.5%) as well as in 5 of 13,207 other tumors (0.04%). These included 1.2% of 91 adenoid cystic carcinomas, 1.2% of 246 mucoepidermoid carcinomas and 0.8% of 127 acinic cell carcinomas of salivary glands. Our data show a good sensitivity (75%) and a high specificity (99.9%) of CELA3B immunohistochemistry for diagnosing acinar cell carcinoma of the pancreas.

AB - Chymotrypsin-like elastase family member 3B (CELA3B, elastase-3B) is a pancreatic enzyme with digestive function in the intestine. Since RNA analyses of normal tissues suggest that CELA3B expression is limited to the pancreas, the potential diagnostic utility of CELA3B immunohistochemistry for the distinction of pancreatic from extrapancreatic cancers and in the distinction of acinar cell carcinoma from ductal adenocarcinoma was assessed. CELA3B expression was successfully analyzed in 13,223 tumor samples from 132 different tumor types and subtypes as well as 8 samples each of 76 different normal tissue types by immunohistochemistry in a tissue microarray format (TMA). In normal tissues, CELA3B immunostaining was only seen in acinar cells and in a fraction of ductal cells of the pancreas as well as on some apical membranes of surface epithelial cells of the intestine. Among tumors, CELA3B immunostaining was seen in 12 of 16 (75%) acinar cell carcinoma of the pancreas including 6 cases with strong staining (37.5%) as well as in 5 of 13,207 other tumors (0.04%). These included 1.2% of 91 adenoid cystic carcinomas, 1.2% of 246 mucoepidermoid carcinomas and 0.8% of 127 acinic cell carcinomas of salivary glands. Our data show a good sensitivity (75%) and a high specificity (99.9%) of CELA3B immunohistochemistry for diagnosing acinar cell carcinoma of the pancreas.

KW - Humans

KW - Carcinoma, Acinar Cell/diagnosis

KW - Pancreas/pathology

KW - Salivary Glands/metabolism

KW - Carcinoma, Adenoid Cystic/metabolism

KW - Pancreatic Elastase/metabolism

KW - Biomarkers, Tumor/metabolism

U2 - 10.1371/journal.pone.0287528

DO - 10.1371/journal.pone.0287528

M3 - SCORING: Journal article

C2 - 37379306

VL - 18

SP - e0287528

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 6

ER -