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Cathepsin G is differentially expressed in primary human antigen-presenting cells.

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Cathepsin G is differentially expressed in primary human antigen-presenting cells. / Stoeckle, Christina; Sommandas, Vinod; Adamopoulou, Eleni; Belisle, Kurt; Schiekofer, Stephan; Melms, Arthur; Weber, Ekkehard; Driessen, Christoph; Fleischer, Bernhard; Tolosa, Eva; Burster, Timo.

In: CELL IMMUNOL, Vol. 255, No. 1-2, 1-2, 2009, p. 41-45.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Stoeckle, C, Sommandas, V, Adamopoulou, E, Belisle, K, Schiekofer, S, Melms, A, Weber, E, Driessen, C, Fleischer, B, Tolosa, E & Burster, T 2009, 'Cathepsin G is differentially expressed in primary human antigen-presenting cells.', CELL IMMUNOL, vol. 255, no. 1-2, 1-2, pp. 41-45. <http://www.ncbi.nlm.nih.gov/pubmed/19036358?dopt=Citation>

APA

Stoeckle, C., Sommandas, V., Adamopoulou, E., Belisle, K., Schiekofer, S., Melms, A., Weber, E., Driessen, C., Fleischer, B., Tolosa, E., & Burster, T. (2009). Cathepsin G is differentially expressed in primary human antigen-presenting cells. CELL IMMUNOL, 255(1-2), 41-45. [1-2]. http://www.ncbi.nlm.nih.gov/pubmed/19036358?dopt=Citation

Vancouver

Stoeckle C, Sommandas V, Adamopoulou E, Belisle K, Schiekofer S, Melms A et al. Cathepsin G is differentially expressed in primary human antigen-presenting cells. CELL IMMUNOL. 2009;255(1-2):41-45. 1-2.

Bibtex

@article{8b8c353ba32c4a11bf87971ae5b6b454,
title = "Cathepsin G is differentially expressed in primary human antigen-presenting cells.",
abstract = "Cathepsins are required for the processing of antigens in order to make them suitable for loading on major histocompatibility complex (MHC) class II molecules, for subsequent presentation to CD4(+) T cells. It was shown that antigen processing in monocyte-derived dendritic cells (DC), a commonly used DC model, is different from that of primary human DC. Here, we report that the two subsets of human myeloid DC (mDC) and plasmacytoid DC (pDC) differ in their cathepsin distribution. The serine protease cathepsin G (CatG) was detected in mDC1, mDC2, pDC, cortical thymic epithelial cells (cTEC) and high levels of CatG were determined in pDC. To address the role of CatG in the processing and presentation of a Multiple Sclerosis-associated autoantigen myelin basic protein (MBP), we used a non-CatG expressing fibroblast cell line and fibroblasts, which were preloaded with purified CatG. We find that preloading fibroblasts with CatG results in a decrease of MBP84-98-specific T cell proliferation, when compared to control cells. Our data suggest a different processing signature in primary human antigen-presenting cells and CatG may be of functional importance.",
author = "Christina Stoeckle and Vinod Sommandas and Eleni Adamopoulou and Kurt Belisle and Stephan Schiekofer and Arthur Melms and Ekkehard Weber and Christoph Driessen and Bernhard Fleischer and Eva Tolosa and Timo Burster",
year = "2009",
language = "Deutsch",
volume = "255",
pages = "41--45",
journal = "CELL IMMUNOL",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1-2",

}

RIS

TY - JOUR

T1 - Cathepsin G is differentially expressed in primary human antigen-presenting cells.

AU - Stoeckle, Christina

AU - Sommandas, Vinod

AU - Adamopoulou, Eleni

AU - Belisle, Kurt

AU - Schiekofer, Stephan

AU - Melms, Arthur

AU - Weber, Ekkehard

AU - Driessen, Christoph

AU - Fleischer, Bernhard

AU - Tolosa, Eva

AU - Burster, Timo

PY - 2009

Y1 - 2009

N2 - Cathepsins are required for the processing of antigens in order to make them suitable for loading on major histocompatibility complex (MHC) class II molecules, for subsequent presentation to CD4(+) T cells. It was shown that antigen processing in monocyte-derived dendritic cells (DC), a commonly used DC model, is different from that of primary human DC. Here, we report that the two subsets of human myeloid DC (mDC) and plasmacytoid DC (pDC) differ in their cathepsin distribution. The serine protease cathepsin G (CatG) was detected in mDC1, mDC2, pDC, cortical thymic epithelial cells (cTEC) and high levels of CatG were determined in pDC. To address the role of CatG in the processing and presentation of a Multiple Sclerosis-associated autoantigen myelin basic protein (MBP), we used a non-CatG expressing fibroblast cell line and fibroblasts, which were preloaded with purified CatG. We find that preloading fibroblasts with CatG results in a decrease of MBP84-98-specific T cell proliferation, when compared to control cells. Our data suggest a different processing signature in primary human antigen-presenting cells and CatG may be of functional importance.

AB - Cathepsins are required for the processing of antigens in order to make them suitable for loading on major histocompatibility complex (MHC) class II molecules, for subsequent presentation to CD4(+) T cells. It was shown that antigen processing in monocyte-derived dendritic cells (DC), a commonly used DC model, is different from that of primary human DC. Here, we report that the two subsets of human myeloid DC (mDC) and plasmacytoid DC (pDC) differ in their cathepsin distribution. The serine protease cathepsin G (CatG) was detected in mDC1, mDC2, pDC, cortical thymic epithelial cells (cTEC) and high levels of CatG were determined in pDC. To address the role of CatG in the processing and presentation of a Multiple Sclerosis-associated autoantigen myelin basic protein (MBP), we used a non-CatG expressing fibroblast cell line and fibroblasts, which were preloaded with purified CatG. We find that preloading fibroblasts with CatG results in a decrease of MBP84-98-specific T cell proliferation, when compared to control cells. Our data suggest a different processing signature in primary human antigen-presenting cells and CatG may be of functional importance.

M3 - SCORING: Zeitschriftenaufsatz

VL - 255

SP - 41

EP - 45

JO - CELL IMMUNOL

JF - CELL IMMUNOL

SN - 0008-8749

IS - 1-2

M1 - 1-2

ER -