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Cas9-expressing chickens and pigs as resources for genome editing in livestock

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Cas9-expressing chickens and pigs as resources for genome editing in livestock. / Rieblinger, Beate; Sid, Hicham; Bozoglu, Tarik; Klinger, Romina; Schlickenrieder, Antonina; Lengyel, Kamila; Flisikowski, Krzysztof; Flisikowska, Tatiana; Simm, Nina; Grodziecki, Alessandro; Perleberg, Carolin; Bähr, Andrea; Carrier, Lucie; Wolf, Eckhard; Kessler, Barbara; Kettler, Lutz; Luksch, Harald; Hagag, Ibrahim T.; Wise, Daniel; Kaufman, Jim; Kaufer, Benedikt B.; Kupatt, Christian; Schnieke, Angelika; Schusser, Benjamin.

In: P NATL ACAD SCI USA, Vol. 118, No. 10, e2022562118, 09.03.2021.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rieblinger, B, Sid, H, Bozoglu, T, Klinger, R, Schlickenrieder, A, Lengyel, K, Flisikowski, K, Flisikowska, T, Simm, N, Grodziecki, A, Perleberg, C, Bähr, A, Carrier, L, Wolf, E, Kessler, B, Kettler, L, Luksch, H, Hagag, IT, Wise, D, Kaufman, J, Kaufer, BB, Kupatt, C, Schnieke, A & Schusser, B 2021, 'Cas9-expressing chickens and pigs as resources for genome editing in livestock', P NATL ACAD SCI USA, vol. 118, no. 10, e2022562118. https://doi.org/10.1073/pnas.2022562118

APA

Rieblinger, B., Sid, H., Bozoglu, T., Klinger, R., Schlickenrieder, A., Lengyel, K., Flisikowski, K., Flisikowska, T., Simm, N., Grodziecki, A., Perleberg, C., Bähr, A., Carrier, L., Wolf, E., Kessler, B., Kettler, L., Luksch, H., Hagag, I. T., Wise, D., ... Schusser, B. (2021). Cas9-expressing chickens and pigs as resources for genome editing in livestock. P NATL ACAD SCI USA, 118(10), [e2022562118]. https://doi.org/10.1073/pnas.2022562118

Vancouver

Rieblinger B, Sid H, Bozoglu T, Klinger R, Schlickenrieder A, Lengyel K et al. Cas9-expressing chickens and pigs as resources for genome editing in livestock. P NATL ACAD SCI USA. 2021 Mar 9;118(10). e2022562118. https://doi.org/10.1073/pnas.2022562118

Bibtex

@article{72d724dda54645089178e9a01c80a14a,
title = "Cas9-expressing chickens and pigs as resources for genome editing in livestock",
abstract = "Genetically modified animals continue to provide important insights into the molecular basis of health and disease. Research has focused mostly on genetically modified mice, although other species like pigs resemble the human physiology more closely. In addition, cross-species comparisons with phylogenetically distant species such as chickens provide powerful insights into fundamental biological and biomedical processes. One of the most versatile genetic methods applicable across species is CRISPR-Cas9. Here, we report the generation of transgenic chickens and pigs that constitutively express Cas9 in all organs. These animals are healthy and fertile. Functionality of Cas9 was confirmed in both species for a number of different target genes, for a variety of cell types and in vivo by targeted gene disruption in lymphocytes and the developing brain, and by precise excision of a 12.7-kb DNA fragment in the heart. The Cas9 transgenic animals will provide a powerful resource for in vivo genome editing for both agricultural and translational biomedical research, and will facilitate reverse genetics as well as cross-species comparisons.",
keywords = "Animals, Animals, Genetically Modified/genetics, CRISPR-Cas Systems, Chickens/genetics, Gene Editing, Livestock/genetics, Swine/genetics",
author = "Beate Rieblinger and Hicham Sid and Tarik Bozoglu and Romina Klinger and Antonina Schlickenrieder and Kamila Lengyel and Krzysztof Flisikowski and Tatiana Flisikowska and Nina Simm and Alessandro Grodziecki and Carolin Perleberg and Andrea B{\"a}hr and Lucie Carrier and Eckhard Wolf and Barbara Kessler and Lutz Kettler and Harald Luksch and Hagag, {Ibrahim T.} and Daniel Wise and Jim Kaufman and Kaufer, {Benedikt B.} and Christian Kupatt and Angelika Schnieke and Benjamin Schusser",
year = "2021",
month = mar,
day = "9",
doi = "10.1073/pnas.2022562118",
language = "English",
volume = "118",
journal = "P NATL ACAD SCI USA",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "10",

}

RIS

TY - JOUR

T1 - Cas9-expressing chickens and pigs as resources for genome editing in livestock

AU - Rieblinger, Beate

AU - Sid, Hicham

AU - Bozoglu, Tarik

AU - Klinger, Romina

AU - Schlickenrieder, Antonina

AU - Lengyel, Kamila

AU - Flisikowski, Krzysztof

AU - Flisikowska, Tatiana

AU - Simm, Nina

AU - Grodziecki, Alessandro

AU - Perleberg, Carolin

AU - Bähr, Andrea

AU - Carrier, Lucie

AU - Wolf, Eckhard

AU - Kessler, Barbara

AU - Kettler, Lutz

AU - Luksch, Harald

AU - Hagag, Ibrahim T.

AU - Wise, Daniel

AU - Kaufman, Jim

AU - Kaufer, Benedikt B.

AU - Kupatt, Christian

AU - Schnieke, Angelika

AU - Schusser, Benjamin

PY - 2021/3/9

Y1 - 2021/3/9

N2 - Genetically modified animals continue to provide important insights into the molecular basis of health and disease. Research has focused mostly on genetically modified mice, although other species like pigs resemble the human physiology more closely. In addition, cross-species comparisons with phylogenetically distant species such as chickens provide powerful insights into fundamental biological and biomedical processes. One of the most versatile genetic methods applicable across species is CRISPR-Cas9. Here, we report the generation of transgenic chickens and pigs that constitutively express Cas9 in all organs. These animals are healthy and fertile. Functionality of Cas9 was confirmed in both species for a number of different target genes, for a variety of cell types and in vivo by targeted gene disruption in lymphocytes and the developing brain, and by precise excision of a 12.7-kb DNA fragment in the heart. The Cas9 transgenic animals will provide a powerful resource for in vivo genome editing for both agricultural and translational biomedical research, and will facilitate reverse genetics as well as cross-species comparisons.

AB - Genetically modified animals continue to provide important insights into the molecular basis of health and disease. Research has focused mostly on genetically modified mice, although other species like pigs resemble the human physiology more closely. In addition, cross-species comparisons with phylogenetically distant species such as chickens provide powerful insights into fundamental biological and biomedical processes. One of the most versatile genetic methods applicable across species is CRISPR-Cas9. Here, we report the generation of transgenic chickens and pigs that constitutively express Cas9 in all organs. These animals are healthy and fertile. Functionality of Cas9 was confirmed in both species for a number of different target genes, for a variety of cell types and in vivo by targeted gene disruption in lymphocytes and the developing brain, and by precise excision of a 12.7-kb DNA fragment in the heart. The Cas9 transgenic animals will provide a powerful resource for in vivo genome editing for both agricultural and translational biomedical research, and will facilitate reverse genetics as well as cross-species comparisons.

KW - Animals

KW - Animals, Genetically Modified/genetics

KW - CRISPR-Cas Systems

KW - Chickens/genetics

KW - Gene Editing

KW - Livestock/genetics

KW - Swine/genetics

U2 - 10.1073/pnas.2022562118

DO - 10.1073/pnas.2022562118

M3 - SCORING: Journal article

C2 - 33658378

VL - 118

JO - P NATL ACAD SCI USA

JF - P NATL ACAD SCI USA

SN - 0027-8424

IS - 10

M1 - e2022562118

ER -